Reviewed, UniProtKB/Swiss-Prot Q9ZPB7 (AL7A1_MALDO)

Names and origin

Protein names	Recommended name:     Aldehyde dehydrogenase family 7 member A1     EC=1.2.1.3 Alternative name(s):     Antiquitin-1     Matured fruit 60 kDa protein       Short name=MF-60
Organism	Malus domestica (Apple) (Malus sylvestris)
Taxonomic identifier	3750 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Rosales › Rosaceae › Maloideae › Malus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in fruit development. Ref.1
Catalytic activity	An aldehyde + NAD+ + H2O = an acid + NADH. UniProtKB P83402
Subunit structure	Homotetramer By similarity. UniProtKB P83402
Developmental stage	Expressed in developing fruit during the enlargement stage. Ref.1
Induction	By osmotic pressure changes Potential. Ref.1
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular aldehyde metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aldehyde dehydrogenase (NAD) activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 508	507	Aldehyde dehydrogenase family 7 member A1		PRO_0000056497
Regions
	Nucleotide binding	244 – 249	6	NAD By similarity UniProtKB Q56694
Sites
	Active site	266	1	Proton acceptor By similarity UniProtKB Q56694
	Active site	300	1	Nucleophile By similarity UniProtKB Q56694
	Site	165	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZPB7-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FCE5487F10DEDABA	FASTA	508	54,244
	10 20 30 40 50 60 MGFAKKEHEF LSAIGLAPEN PGGFINGKWK ASGPVISTVS PSNNQEIAKV TEVSMEEYEE 70 80 90 100 110 120 GLRSCNDAAK TWKSLPAPKR GEIVRQIGDA LREKLQHLGK LVSLEMGKIL AEGIGEVQEV 130 140 150 160 170 180 IYMCDFAVGL SRQLNGSIIP SERPDHMMFE VWNPLGIVGV ITAFNFPCAV LGWNACIALV 190 200 210 220 230 240 CGNCVVWKGA PTTPLVTIAV TKLIAEVLEK NNLPAAIFTA FCGGAEIGEA IAKDTRIPLV 250 260 270 280 290 300 SFTGSSKVGA KVQQIVTERF GKCLLELSGN NALIVMDDAD VGLAVRSIFF AAVGTAGQRC 310 320 330 340 350 360 TTCRRLYLHE SIYQNVLDKL VGLYNQVKIG DPLEEGTLVG PVHTKASREN FEKGISTIKS 370 380 390 400 410 420 QGGKILTGGS VIESDGNFVQ PTIVEIASNA SVVKEELFGP VLYVMKFKTL EEAIALNNSV 430 440 450 460 470 480 PQGLSSSIFT SKPNTIFKWI GPHGSDCGIV NVNIPTNGAE IGGAFGGEKA TGGGREAGSD 490 500 SWKQYMRRST CTINYGTELP LAQGINFG

References

[1]

"Identification and cDNA cloning of a protein abundantly expressed during apple fruit development." Yamada K., Mori H., Yamaki S. Plant Cell Physiol. 40:198-204(1999) [PubMed: 10202815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-31 AND 386-404, INDUCTION. Strain: cv. Fuji. Tissue: Fruit.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D88434 mRNA. Translation: BAA75633.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.3. 66979.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL7A1_MALDO

Accession

Primary (citable) accession number: Q9ZPB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 49 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)