Reviewed, UniProtKB/Swiss-Prot Q9ZP54 (PARP1_ARATH)

Names and origin

Protein names

Recommended name:
    Poly [ADP-ribose] polymerase 1
      Short name=PARP-1
    EC=2.4.2.30
Alternative name(s):
    ADPRT 1
    NAD(+) ADP-ribosyltransferase 1
    Poly[ADP-ribose] synthetase 1

Gene names

Name:	PARP1
Ordered Locus Names:	At2g31320
ORF Names:	F16D14.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	983 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks By similarity.
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subcellular location	Nucleus Potential.
Induction	By ionising radiation (IR)-induced DNA damage. Ref.1
Sequence similarities	Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 2 PARP-type zinc fingers.
Sequence caution	The sequence AAD20677.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding NAD Zinc
Molecular function	Glycosyltransferase Transferase
PTM	ADP-ribosylation
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Ref.1 Inferred from expression pattern. Source: TAIR protein amino acid ADP-ribosylation Inferred from electronic annotation. Source: InterPro response to abiotic stimulus Inferred from mutant phenotype. Source: TAIR response to abscisic acid stimulus Inferred from expression pattern. Source: TAIR response to oxidative stress Inferred from expression pattern. Source: TAIR
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW NAD or NADH binding Inferred from electronic annotation. Source: InterPro NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 983

983

Poly [ADP-ribose] polymerase 1

PRO_0000211331

Regions

Domain

394 – 484

91

BRCT

Domain

633 – 751

119

PARP alpha-helical

Domain

758 – 983

226

PARP catalytic

Zinc finger

8 – 91

84

PARP-type 1

Zinc finger

114 – 194

81

PARP-type 2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

983

Helix Strand Turn

Details...

Beta strand

7 – 12

6

Beta strand

21 – 23

3

Beta strand

29 – 36

8

Beta strand

41 – 44

4

Beta strand

49 – 52

4

Helix

53 – 57

5

Beta strand

59 – 61

3

Helix

66 – 68

3

Turn

71 – 74

4

Helix

77 – 87

11

Beta strand

91 – 93

3

Sequences

	Sequence		Length	Mass (Da)
	Q9ZP54-1 [UniParc]. Last modified December 1, 2001. Version 2. Checksum: 468E12A8EF1B6F4F	FASTA	983	111,233
	10 20 30 40 50 60 MASPHKPWRA EYAKSSRSSC KTCKSVINKE NFRLGKLVQS THFDGIMPMW NHASCILKKT 70 80 90 100 110 120 KQIKSVDDVE GIESLRWEDQ QKIRKYVESG AGSNTSTSTG TSTSSTANNA KLEYGIEVSQ 130 140 150 160 170 180 TSRAGCRKCS EKILKGEVRI FSKPEGPGNK GLMWHHAKCF LEMSSSTELE SLSGWRSIPD 190 200 210 220 230 240 SDQEALLPLV KKALPAAKTE TAEARQTNSR AGTKRKNDSV DNEKSKLAKS SFDMSTSGAL 250 260 270 280 290 300 QPCSKEKEME AQTKELWDLK DDLKKYVTSA ELREMLEVNE QSTRGSELDL RDKCADGMMF 310 320 330 340 350 360 GPLALCPMCS GHLSFSGGLY RCHGYISEWS KCSHSTLDPD RIKGKWKIPD ETENQFLLKW 370 380 390 400 410 420 NKSQKSVKPK RILRPVLSGE TSQGQGSKDA TDSSRSERLA DLKVSIAGNT KERQPWKKRI 430 440 450 460 470 480 EEAGAEFHAN VKKGTSCLVV CGLTDIRDAE MRKARRMKVA IVREDYLVDC FKKQRKLPFD 490 500 510 520 530 540 KYKIEDTSES LVTVKVKGRS AVHEASGLQE HCHILEDGNS IYNTTLSMSD LSTGINSYYI 550 560 570 580 590 600 LQIIQEDKGS DCYVFRKWGR VGNEKIGGNK VEEMSKSDAV HEFKRLFLEK TGNTWESWEQ 610 620 630 640 650 660 KTNFQKQPGK FLPLDIDYGV NKQVAKKEPF QTSSNLAPSL IELMKMLFDV ETYRSAMMEF 670 680 690 700 710 720 EINMSEMPLG KLSKHNIQKG FEALTEIQRL LTESDPQPTM KESLLVDASN RFFTMIPSIH 730 740 750 760 770 780 PHIIRDEDDF KSKVKMLEAL QDIEIASRIV GFDVDSTESL DDKYKKLHCD ISPLPHDSED 790 800 810 820 830 840 YRLIEKYLNT THAPTHTEWS LELEEVFALE REGEFDKYAP HREKLGNKML LWHGSRLTNF 850 860 870 880 890 900 VGILNQGLRI APPEAPATGY MFGKGIYFAD LVSKSAQYCY TCKKNPVGLM LLSEVALGEI 910 920 930 940 950 960 HELTKAKYMD KPPRGKHSTK GLGKKVPQDS EFAKWRGDVT VPCGKPVSSK VKASELMYNE 970 980 YIVYDTAQVK LQFLLKVRFK HKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ionising radiation induces the expression of PARP-1 and PARP-2 genes in Arabidopsis." Doucet-Chabeaud G., Godon C., Brutesco C., de Murcia G., Kazmaier M. Mol. Genet. Genomics 265:954-963(2001) [PubMed: 11523787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Solution structure of the first Zn-finger domain of poly(ADP-ribose) polymerase-1." RIKEN structural genomics initiative (RSGI) Submitted (FEB-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 5-105.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AJ131705 mRNA. Translation: CAA10482.1.
AC006593 Genomic DNA. Translation: AAD20677.1. Sequence problems.
AY091061 mRNA. Translation: AAM13882.1.
AY150460 mRNA. Translation: AAN12901.1.

IPI

IPI00537729.

PIR

C84719.
T51353.

RefSeq

NP_850165.1.

UniGene

At.65741

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V9X	NMR	-	A	5-105	[»]

SMR

Q9ZP54. Positions 2-91.

ModBase

Search...

Proteomic databases

PRIDE

Q9ZP54.

Genome annotation databases

GeneID

817690.

GenomeReviews

Gene locus AT2G31320 in contig CT485783_GR.

KEGG

ath:AT2G31320.

NMPDR

fig|3702.1.peg.29110.

Organism-specific databases

GeneFarm

4815. 467.

TAIR

At2g31320.

Phylogenomic databases

OMA

VDIVKGT.

Enzyme and pathway databases

BRENDA

2.4.2.30. 302.

Gene expression databases

Genevestigator

Q9ZP54.

GermOnline

AT2G31320. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR001357. BRCT.
IPR008288. NAD_ADPRT.
IPR012982. PADR1.
IPR012317. PARP_catalytic.
IPR004102. PARP_reg.
IPR008893. WGR.
IPR001510. Znf_PARP.
[Graphical view]

Gene3D

G3DSA:1.20.142.10. PARP_reg. 1 hit.
G3DSA:3.30.1740.10. Znf_PARP. 2 hits.

Pfam

PF00533. BRCT. 1 hit.
PF08063. PADR1. 1 hit.
PF00644. PARP. 1 hit.
PF02877. PARP_reg. 1 hit.
PF05406. WGR. 1 hit.
PF00645. zf-PARP. 2 hits.
[Graphical view]

PIRSF

PIRSF000489. NAD_ADPRT. 1 hit.

ProDom

PD004675. Znf_PolyADPpol. 2 hits.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00292. BRCT. 1 hit.
SM00773. WGR. 1 hit.
[Graphical view]

PROSITE

PS50172. BRCT. 1 hit.
PS51060. PARP_ALPHA_HD. 1 hit.
PS51059. PARP_CATALYTIC. 1 hit.
PS50064. PARP_ZN_FINGER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PARP1_ARATH

Accession

Primary (citable) accession number: Q9ZP54
Secondary accession number(s): Q9SJW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)