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Protein

Poly [ADP-ribose] polymerase 1

Gene

PARP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (By similarity).By similarity

Catalytic activityi

NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri8 – 9184PARP-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri114 – 19481PARP-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • DNA ligation involved in DNA repair Source: GO_Central
  • DNA repair Source: TAIR
  • lagging strand elongation Source: GO_Central
  • protein ADP-ribosylation Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

DNA-binding, Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G31320-MONOMER.
ReactomeiR-ATH-110362. POLB-Dependent Long Patch Base Excision Repair.
R-ATH-3108214. SUMOylation of DNA damage response and repair proteins.
R-ATH-5685939. HDR through MMEJ (alt-NHEJ).
R-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-5696395. Formation of Incision Complex in GG-NER.
R-ATH-5696400. Dual Incision in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly [ADP-ribose] polymerase 1 (EC:2.4.2.30)
Short name:
PARP-1
Alternative name(s):
NAD(+) ADP-ribosyltransferase 1
Short name:
ADPRT-1
Poly[ADP-ribose] synthase 1
Gene namesi
Name:PARP1
Ordered Locus Names:At2g31320
ORF Names:F16D14.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G31320.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 983983Poly [ADP-ribose] polymerase 1PRO_0000211331Add
BLAST

Keywords - PTMi

ADP-ribosylation

Proteomic databases

PaxDbiQ9ZP54.
PRIDEiQ9ZP54.

PTM databases

iPTMnetiQ9ZP54.

Expressioni

Inductioni

By ionising radiation (IR)-induced DNA damage.1 Publication

Gene expression databases

GenevisibleiQ9ZP54. AT.

Interactioni

Protein-protein interaction databases

BioGridi3038. 1 interaction.
STRINGi3702.AT2G31320.1.

Structurei

Secondary structure

1
983
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Beta strandi21 – 233Combined sources
Beta strandi29 – 368Combined sources
Beta strandi41 – 444Combined sources
Beta strandi49 – 524Combined sources
Helixi53 – 575Combined sources
Beta strandi59 – 613Combined sources
Helixi66 – 683Combined sources
Turni71 – 744Combined sources
Helixi77 – 8711Combined sources
Beta strandi91 – 933Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V9XNMR-A5-105[»]
ProteinModelPortaliQ9ZP54.
SMRiQ9ZP54. Positions 2-91, 114-375, 500-982.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ZP54.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini394 – 48491BRCTPROSITE-ProRule annotationAdd
BLAST
Domaini633 – 751119PARP alpha-helicalPROSITE-ProRule annotationAdd
BLAST
Domaini758 – 983226PARP catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BRCT domain.PROSITE-ProRule annotation
Contains 1 PARP alpha-helical domain.PROSITE-ProRule annotation
Contains 1 PARP catalytic domain.PROSITE-ProRule annotation
Contains 2 PARP-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri8 – 9184PARP-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri114 – 19481PARP-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1037. Eukaryota.
ENOG410XP18. LUCA.
HOGENOMiHOG000030402.
InParanoidiQ9ZP54.
KOiK10798.
OMAiHCHILED.
PhylomeDBiQ9ZP54.

Family and domain databases

Gene3Di1.20.142.10. 1 hit.
3.30.1740.10. 2 hits.
3.40.50.10190. 1 hit.
3.90.228.10. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR012982. PADR1.
IPR008288. PARP.
IPR012317. Poly(ADP-ribose)pol_cat_dom.
IPR004102. Poly(ADP-ribose)pol_reg_dom.
IPR008893. WGR_domain.
IPR001510. Znf_PARP.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF08063. PADR1. 1 hit.
PF00644. PARP. 1 hit.
PF02877. PARP_reg. 1 hit.
PF05406. WGR. 1 hit.
PF00645. zf-PARP. 2 hits.
[Graphical view]
PIRSFiPIRSF000489. NAD_ADPRT. 1 hit.
SMARTiSM00292. BRCT. 1 hit.
SM01335. PADR1. 1 hit.
SM00773. WGR. 1 hit.
SM01336. zf-PARP. 2 hits.
[Graphical view]
SUPFAMiSSF142921. SSF142921. 1 hit.
SSF47587. SSF47587. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS51060. PARP_ALPHA_HD. 1 hit.
PS51059. PARP_CATALYTIC. 1 hit.
PS50064. PARP_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZP54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPHKPWRA EYAKSSRSSC KTCKSVINKE NFRLGKLVQS THFDGIMPMW
60 70 80 90 100
NHASCILKKT KQIKSVDDVE GIESLRWEDQ QKIRKYVESG AGSNTSTSTG
110 120 130 140 150
TSTSSTANNA KLEYGIEVSQ TSRAGCRKCS EKILKGEVRI FSKPEGPGNK
160 170 180 190 200
GLMWHHAKCF LEMSSSTELE SLSGWRSIPD SDQEALLPLV KKALPAAKTE
210 220 230 240 250
TAEARQTNSR AGTKRKNDSV DNEKSKLAKS SFDMSTSGAL QPCSKEKEME
260 270 280 290 300
AQTKELWDLK DDLKKYVTSA ELREMLEVNE QSTRGSELDL RDKCADGMMF
310 320 330 340 350
GPLALCPMCS GHLSFSGGLY RCHGYISEWS KCSHSTLDPD RIKGKWKIPD
360 370 380 390 400
ETENQFLLKW NKSQKSVKPK RILRPVLSGE TSQGQGSKDA TDSSRSERLA
410 420 430 440 450
DLKVSIAGNT KERQPWKKRI EEAGAEFHAN VKKGTSCLVV CGLTDIRDAE
460 470 480 490 500
MRKARRMKVA IVREDYLVDC FKKQRKLPFD KYKIEDTSES LVTVKVKGRS
510 520 530 540 550
AVHEASGLQE HCHILEDGNS IYNTTLSMSD LSTGINSYYI LQIIQEDKGS
560 570 580 590 600
DCYVFRKWGR VGNEKIGGNK VEEMSKSDAV HEFKRLFLEK TGNTWESWEQ
610 620 630 640 650
KTNFQKQPGK FLPLDIDYGV NKQVAKKEPF QTSSNLAPSL IELMKMLFDV
660 670 680 690 700
ETYRSAMMEF EINMSEMPLG KLSKHNIQKG FEALTEIQRL LTESDPQPTM
710 720 730 740 750
KESLLVDASN RFFTMIPSIH PHIIRDEDDF KSKVKMLEAL QDIEIASRIV
760 770 780 790 800
GFDVDSTESL DDKYKKLHCD ISPLPHDSED YRLIEKYLNT THAPTHTEWS
810 820 830 840 850
LELEEVFALE REGEFDKYAP HREKLGNKML LWHGSRLTNF VGILNQGLRI
860 870 880 890 900
APPEAPATGY MFGKGIYFAD LVSKSAQYCY TCKKNPVGLM LLSEVALGEI
910 920 930 940 950
HELTKAKYMD KPPRGKHSTK GLGKKVPQDS EFAKWRGDVT VPCGKPVSSK
960 970 980
VKASELMYNE YIVYDTAQVK LQFLLKVRFK HKR
Length:983
Mass (Da):111,233
Last modified:December 1, 2001 - v2
Checksum:i468E12A8EF1B6F4F
GO

Sequence cautioni

The sequence AAD20677.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ131705 mRNA. Translation: CAA10482.1.
AC006593 Genomic DNA. Translation: AAD20677.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08527.1.
AY091061 mRNA. Translation: AAM13882.1.
AY150460 mRNA. Translation: AAN12901.1.
PIRiC84719.
T51353.
RefSeqiNP_850165.1. NM_179834.1.
UniGeneiAt.65741.

Genome annotation databases

EnsemblPlantsiAT2G31320.1; AT2G31320.1; AT2G31320.
GeneIDi817690.
GrameneiAT2G31320.1; AT2G31320.1; AT2G31320.
KEGGiath:AT2G31320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ131705 mRNA. Translation: CAA10482.1.
AC006593 Genomic DNA. Translation: AAD20677.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08527.1.
AY091061 mRNA. Translation: AAM13882.1.
AY150460 mRNA. Translation: AAN12901.1.
PIRiC84719.
T51353.
RefSeqiNP_850165.1. NM_179834.1.
UniGeneiAt.65741.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V9XNMR-A5-105[»]
ProteinModelPortaliQ9ZP54.
SMRiQ9ZP54. Positions 2-91, 114-375, 500-982.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3038. 1 interaction.
STRINGi3702.AT2G31320.1.

PTM databases

iPTMnetiQ9ZP54.

Proteomic databases

PaxDbiQ9ZP54.
PRIDEiQ9ZP54.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G31320.1; AT2G31320.1; AT2G31320.
GeneIDi817690.
GrameneiAT2G31320.1; AT2G31320.1; AT2G31320.
KEGGiath:AT2G31320.

Organism-specific databases

TAIRiAT2G31320.

Phylogenomic databases

eggNOGiKOG1037. Eukaryota.
ENOG410XP18. LUCA.
HOGENOMiHOG000030402.
InParanoidiQ9ZP54.
KOiK10798.
OMAiHCHILED.
PhylomeDBiQ9ZP54.

Enzyme and pathway databases

BioCyciARA:AT2G31320-MONOMER.
ReactomeiR-ATH-110362. POLB-Dependent Long Patch Base Excision Repair.
R-ATH-3108214. SUMOylation of DNA damage response and repair proteins.
R-ATH-5685939. HDR through MMEJ (alt-NHEJ).
R-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-5696395. Formation of Incision Complex in GG-NER.
R-ATH-5696400. Dual Incision in GG-NER.

Miscellaneous databases

EvolutionaryTraceiQ9ZP54.
PROiQ9ZP54.

Gene expression databases

GenevisibleiQ9ZP54. AT.

Family and domain databases

Gene3Di1.20.142.10. 1 hit.
3.30.1740.10. 2 hits.
3.40.50.10190. 1 hit.
3.90.228.10. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR012982. PADR1.
IPR008288. PARP.
IPR012317. Poly(ADP-ribose)pol_cat_dom.
IPR004102. Poly(ADP-ribose)pol_reg_dom.
IPR008893. WGR_domain.
IPR001510. Znf_PARP.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF08063. PADR1. 1 hit.
PF00644. PARP. 1 hit.
PF02877. PARP_reg. 1 hit.
PF05406. WGR. 1 hit.
PF00645. zf-PARP. 2 hits.
[Graphical view]
PIRSFiPIRSF000489. NAD_ADPRT. 1 hit.
SMARTiSM00292. BRCT. 1 hit.
SM01335. PADR1. 1 hit.
SM00773. WGR. 1 hit.
SM01336. zf-PARP. 2 hits.
[Graphical view]
SUPFAMiSSF142921. SSF142921. 1 hit.
SSF47587. SSF47587. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS51060. PARP_ALPHA_HD. 1 hit.
PS51059. PARP_CATALYTIC. 1 hit.
PS50064. PARP_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Ionising radiation induces the expression of PARP-1 and PARP-2 genes in Arabidopsis."
    Doucet-Chabeaud G., Godon C., Brutesco C., de Murcia G., Kazmaier M.
    Mol. Genet. Genomics 265:954-963(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    Strain: cv. Landsberg erecta.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Solution structure of the first Zn-finger domain of poly(ADP-ribose) polymerase-1."
    RIKEN structural genomics initiative (RSGI)
    Submitted (FEB-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 5-105.

Entry informationi

Entry nameiPARP1_ARATH
AccessioniPrimary (citable) accession number: Q9ZP54
Secondary accession number(s): Q9SJW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.