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Protein

Polyphenol oxidase I, chloroplastic

Gene

co-1

Organism
Ipomoea batatas (Sweet potato) (Convolvulus batatas)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.

Catalytic activityi

2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.

Cofactori

Cu2+1 PublicationNote: Binds 2 copper ions per subunit.1 Publication

Enzyme regulationi

Inhibited by phenylthiourea.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi88Copper A1 Publication1
Metal bindingi109Copper A1 Publication1
Metal bindingi118Copper A1 Publication1
Metal bindingi240Copper B1 Publication1
Metal bindingi244Copper B1 Publication1
Metal bindingi274Copper B1 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BRENDAi1.10.3.1. 2773.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphenol oxidase I, chloroplastic (EC:1.10.3.1)
Short name:
PPO-I
Alternative name(s):
Catechol oxidase I
Gene namesi
Name:co-1
OrganismiIpomoea batatas (Sweet potato) (Convolvulus batatas)
Taxonomic identifieri4120 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesConvolvulaceaeIpomoeeaeIpomoea

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001867411 – 496Polyphenol oxidase I, chloroplasticAdd BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi11 ↔ 281 Publication
Disulfide bondi27 ↔ 891 Publication
Cross-linki92 ↔ 1092'-(S-cysteinyl)-histidine (Cys-His)1 Publication

Keywords - PTMi

Disulfide bond, Thioether bond

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 10Combined sources3
Beta strandi33 – 37Combined sources5
Beta strandi47 – 49Combined sources3
Helixi52 – 54Combined sources3
Helixi57 – 71Combined sources15
Helixi81 – 92Combined sources12
Beta strandi96 – 98Combined sources3
Beta strandi101 – 105Combined sources5
Beta strandi109 – 111Combined sources3
Helixi114 – 133Combined sources20
Helixi148 – 150Combined sources3
Helixi155 – 158Combined sources4
Beta strandi167 – 169Combined sources3
Helixi171 – 173Combined sources3
Helixi192 – 207Combined sources16
Turni208 – 210Combined sources3
Helixi214 – 218Combined sources5
Helixi234 – 237Combined sources4
Helixi240 – 247Combined sources8
Turni256 – 259Combined sources4
Turni261 – 263Combined sources3
Helixi264 – 266Combined sources3
Helixi269 – 287Combined sources19
Helixi299 – 302Combined sources4
Beta strandi305 – 309Combined sources5
Beta strandi315 – 319Combined sources5
Helixi320 – 322Combined sources3
Helixi326 – 329Combined sources4
Beta strandi331 – 333Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BT1X-ray2.70A/B1-345[»]
1BT2X-ray2.70A/B1-345[»]
1BT3X-ray2.50A1-345[»]
1BUGX-ray2.70A/B1-345[»]
ProteinModelPortaliQ9ZP19.
SMRiQ9ZP19.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ZP19.

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZP19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
APIQAPEISK CVVPPADLPP GAVVDNCCPP VASNIVDYKL PAVTTMKVRP
60 70 80 90 100
AAHTMDKDAI AKFAKAVELM KALPADDPRN FYQQALVHCA YCNGGYDQVN
110 120 130 140 150
FPDQEIQVHN SWLFFPFHRW YLYFYERILG KLIGDPSFGL PFWNWDNPGG
160 170 180 190 200
MVLPDFLNDS TSSLYDSNRN QSHLPPVVVD LGYNGADTDV TDQQRITDNL
210 220 230 240 250
ALMYKQMVTN AGTAELFLGK AYRAGDAPSP GAGSIETSPH IPIHRWVGDP
260 270 280 290 300
RNTNNEDMGN FYSAGRDIAF YCHHSNVDRM WTIWQQLAGK PRKRDYTDSD
310 320 330 340 350
WLNATFLFYD ENGQAVKVRI GDSLDNQKMG YKYAKTPLPW LDSKPVPTKK
360 370 380 390 400
KGGYASKSKA PFVASVFPVT LDKVVQVKVA RPKKSRSAEE KEAEEEILLI
410 420 430 440 450
VGIEVEIDKY AKFDVYLNDS DDPSGGKDKA EYAGSFAHLP HKHKGMKKIR
460 470 480 490
TTLSLGLNEP LEDLGAEDDD TILVTLAPKV GGGVVSVDNV KVVYGS
Length:496
Mass (Da):55,011
Last modified:May 1, 1999 - v1
Checksum:i806717DBF5B09705
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40 – 42LPA → IPV in CAC83609 (Ref. 2) Curated3
Sequence conflicti65K → R in CAC83609 (Ref. 2) Curated1
Sequence conflicti68E → D in CAC83609 (Ref. 2) Curated1
Sequence conflicti71K → R in CAC83609 (Ref. 2) Curated1
Sequence conflicti75A → G in CAC83609 (Ref. 2) Curated1
Sequence conflicti282T → A in CAC83609 (Ref. 2) Curated1
Sequence conflicti401 – 403VGI → EGV in CAC83609 (Ref. 2) Curated3
Sequence conflicti436F → S in CAC83609 (Ref. 2) Curated1
Sequence conflicti475T → A in CAC83609 (Ref. 2) Curated1
Sequence conflicti490V → I in CAC83609 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006097 mRNA. Translation: CAA06855.1.
AJ309175 Genomic DNA. Translation: CAC83609.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006097 mRNA. Translation: CAA06855.1.
AJ309175 Genomic DNA. Translation: CAC83609.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BT1X-ray2.70A/B1-345[»]
1BT2X-ray2.70A/B1-345[»]
1BT3X-ray2.50A1-345[»]
1BUGX-ray2.70A/B1-345[»]
ProteinModelPortaliQ9ZP19.
SMRiQ9ZP19.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi1.10.3.1. 2773.

Miscellaneous databases

EvolutionaryTraceiQ9ZP19.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPO1_IPOBA
AccessioniPrimary (citable) accession number: Q9ZP19
Secondary accession number(s): Q84V53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.