Reviewed,
UniProtKB/Swiss-Prot Q9ZP06 (MDHM1_ARATH)
Last modified
June 16, 2009.
Version 91.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Malate dehydrogenase 1, mitochondrial EC=1.1.1.37 Alternative name(s): mNAD-MDH 1 | ||||
| Gene names |
| ||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||
| Taxonomic identifier | 3702 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 341 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD+ = oxaloacetate + NADH. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | |
| Sequence similarities | Belongs to the LDH/MDH superfamily. MDH type 1 family. |
| Sequence caution | The sequence AAF69549.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 22 | 22 | Mitochondrion Ref.5 | ||||||
| Chain | 23 – 341 | 319 | Malate dehydrogenase 1, mitochondrial | PRO_0000018622 | |||||
Regions | |||||||||
| Nucleotide binding | 36 – 42 | 7 | NAD By similarity | ||||||
| Nucleotide binding | 145 – 147 | 3 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 205 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 62 | 1 | NAD By similarity | ||||||
| Binding site | 109 | 1 | Substrate By similarity | ||||||
| Binding site | 115 | 1 | Substrate By similarity | ||||||
| Binding site | 122 | 1 | NAD By similarity | ||||||
| Binding site | 147 | 1 | Substrate By similarity | ||||||
| Binding site | 181 | 1 | Substrate By similarity | ||||||
| Binding site | 256 | 1 | NAD By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 17 | 1 | V → A in AAM64855. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A novel, non-redox-regulated NAD-dependent malate dehydrogenase from chloroplasts of Arabidopsis thaliana L." Berkemeyer M., Scheibe R., Ocheretina O. J. Biol. Chem. 273:27927-27933(1998) [PubMed: 9774405] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [5] | "Proteomic approach to identify novel mitochondrial proteins in Arabidopsis." Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P. Plant Physiol. 127:1694-1710(2001) [PubMed: 11743114] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-37, SUBCELLULAR LOCATION. Tissue: Leaf and Stem. |
| [6] | "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
Cross-references
Sequence databases | |
|---|---|
| AJ131205 mRNA. Translation: CAA10320.1. AC008007 Genomic DNA. Translation: AAF69549.1. Sequence problems. AF324670 mRNA. Translation: AAG40021.1. AF339684 mRNA. Translation: AAK00366.1. AY062580 mRNA. Translation: AAL32658.1. AY128783 mRNA. Translation: AAM91183.1. AY087304 mRNA. Translation: AAM64855.1. | |
| IPI | IPI00543566. |
| PIR | T51311. |
| RefSeq | NP_564625.1. |
| UniGene | At.23771 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1MLD based on UniProtKB P00346. |
| ModBase | Search... |
2-D gel databases | |
| SWISS-2DPAGE | Q9ZP06. |
Proteomic databases | |
| PRIDE | Q9ZP06. |
| ProMEX | Q9ZP06. |
Genome annotation databases | |
| GeneID | 841757. |
| GenomeReviews | Gene locus AT1G53240 in contig CT485782_GR. |
| KEGG | ath:AT1G53240. |
| NMPDR | fig|3702.1.peg.4820. |
Organism-specific databases | |
| GeneFarm | 2023. 159. |
| TAIR | At1g53240. |
Phylogenomic databases | |
| OMA | Q9ZP06. CSYVQST. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.37. 302. |
Gene expression databases | |
| ArrayExpress | Q9ZP06. |
| GermOnline | AT1G53240. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR11540:SF1. MDH_euk_g_bac. 1 hit. |
| Pfam | PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000102. Lac_mal_DH. 1 hit. |
| TIGRFAMs | TIGR01772. MDH_euk_gproteo. 1 hit. |
| PROSITE | PS00068. MDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MDHM1_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9ZP06 Secondary accession number(s): Q8LBB9, Q9MAH7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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