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Q9ZP06

- MDHM1_ARATH

UniProt

Q9ZP06 - MDHM1_ARATH

Protein

Malate dehydrogenase 1, mitochondrial

Gene

At1g53240

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei62 – 621NADBy similarity
    Binding sitei109 – 1091SubstratePROSITE-ProRule annotation
    Binding sitei115 – 1151SubstratePROSITE-ProRule annotation
    Binding sitei122 – 1221NADBy similarity
    Binding sitei147 – 1471SubstratePROSITE-ProRule annotation
    Binding sitei181 – 1811SubstratePROSITE-ProRule annotation
    Active sitei205 – 2051Proton acceptorBy similarity
    Binding sitei256 – 2561NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi36 – 427NADBy similarity
    Nucleotide bindingi145 – 1473NADBy similarity

    GO - Molecular functioni

    1. copper ion binding Source: TAIR
    2. L-malate dehydrogenase activity Source: TAIR

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. defense response to bacterium Source: TAIR
    3. malate metabolic process Source: InterPro
    4. response to cadmium ion Source: TAIR
    5. response to cold Source: TAIR
    6. response to salt stress Source: TAIR
    7. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciARA:AT1G53240-MONOMER.
    MetaCyc:AT1G53240-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase 1, mitochondrial (EC:1.1.1.37)
    Alternative name(s):
    mNAD-MDH 1
    Gene namesi
    Ordered Locus Names:At1g53240
    ORF Names:F12M16.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G53240.

    Subcellular locationi

    Mitochondrion matrix 2 Publications

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. cell wall Source: TAIR
    3. chloroplast Source: TAIR
    4. mitochondrial matrix Source: UniProtKB-SubCell
    5. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2222Mitochondrion1 PublicationAdd
    BLAST
    Chaini23 – 341319Malate dehydrogenase 1, mitochondrialPRO_0000018622Add
    BLAST

    Proteomic databases

    PaxDbiQ9ZP06.
    PRIDEiQ9ZP06.

    2D gel databases

    SWISS-2DPAGEQ9ZP06.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9ZP06.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi26982. 1 interaction.
    IntActiQ9ZP06. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZP06.
    SMRiQ9ZP06. Positions 31-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213792.
    InParanoidiQ9ZP06.
    KOiK00026.
    OMAiYVQSTIT.
    PhylomeDBiQ9ZP06.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9ZP06-1 [UniParc]FASTAAdd to Basket

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    MFRSMLVRSS ASAKQAVIRR SFSSGSVPER KVAILGAAGG IGQPLALLMK    50
    LNPLVSSLSL YDIANTPGVA ADVGHINTRS EVVGYMGDDN LAKALEGADL 100
    VIIPAGVPRK PGMTRDDLFN INAGIVKNLC TAIAKYCPHA LINMISNPVN 150
    STVPIAAEIF KKAGMYDEKK LFGVTTLDVV RARTFYAGKA NVPVAEVNVP 200
    VIGGHAGVTI LPLFSQATPQ ANLSSDILTA LTKRTQDGGT EVVEAKAGKG 250
    SATLSMAYAG ALFADACLKG LNGVPDVIEC SYVQSTITEL PFFASKVRLG 300
    KNGVEEVLDL GPLSDFEKEG LEALKPELKS SIEKGVKFAN Q 341
    Length:341
    Mass (Da):35,804
    Last modified:May 1, 1999 - v1
    Checksum:iD035C4C38785BE57
    GO

    Sequence cautioni

    The sequence AAF69549.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171V → A in AAM64855. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ131205 mRNA. Translation: CAA10320.1.
    AC008007 Genomic DNA. Translation: AAF69549.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32910.1.
    AF324670 mRNA. Translation: AAG40021.1.
    AF339684 mRNA. Translation: AAK00366.1.
    AY062580 mRNA. Translation: AAL32658.1.
    AY128783 mRNA. Translation: AAM91183.1.
    AY087304 mRNA. Translation: AAM64855.1.
    PIRiT51311.
    RefSeqiNP_564625.1. NM_104202.2.
    UniGeneiAt.23771.

    Genome annotation databases

    EnsemblPlantsiAT1G53240.1; AT1G53240.1; AT1G53240.
    GeneIDi841757.
    KEGGiath:AT1G53240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ131205 mRNA. Translation: CAA10320.1 .
    AC008007 Genomic DNA. Translation: AAF69549.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32910.1 .
    AF324670 mRNA. Translation: AAG40021.1 .
    AF339684 mRNA. Translation: AAK00366.1 .
    AY062580 mRNA. Translation: AAL32658.1 .
    AY128783 mRNA. Translation: AAM91183.1 .
    AY087304 mRNA. Translation: AAM64855.1 .
    PIRi T51311.
    RefSeqi NP_564625.1. NM_104202.2.
    UniGenei At.23771.

    3D structure databases

    ProteinModelPortali Q9ZP06.
    SMRi Q9ZP06. Positions 31-338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 26982. 1 interaction.
    IntActi Q9ZP06. 1 interaction.

    2D gel databases

    SWISS-2DPAGE Q9ZP06.

    Proteomic databases

    PaxDbi Q9ZP06.
    PRIDEi Q9ZP06.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G53240.1 ; AT1G53240.1 ; AT1G53240 .
    GeneIDi 841757.
    KEGGi ath:AT1G53240.

    Organism-specific databases

    GeneFarmi 2023. 159.
    TAIRi AT1G53240.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213792.
    InParanoidi Q9ZP06.
    KOi K00026.
    OMAi YVQSTIT.
    PhylomeDBi Q9ZP06.

    Enzyme and pathway databases

    BioCyci ARA:AT1G53240-MONOMER.
    MetaCyc:AT1G53240-MONOMER.

    Gene expression databases

    Genevestigatori Q9ZP06.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel, non-redox-regulated NAD-dependent malate dehydrogenase from chloroplasts of Arabidopsis thaliana L."
      Berkemeyer M., Scheibe R., Ocheretina O.
      J. Biol. Chem. 273:27927-27933(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Proteomic approach to identify novel mitochondrial proteins in Arabidopsis."
      Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.
      Plant Physiol. 127:1694-1710(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 23-37, SUBCELLULAR LOCATION.
      Tissue: Leaf and Stem.
    7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.
    8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMDHM1_ARATH
    AccessioniPrimary (citable) accession number: Q9ZP06
    Secondary accession number(s): Q8LBB9, Q9MAH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3