SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9ZP06

- MDHM1_ARATH

UniProt

Q9ZP06 - MDHM1_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Malate dehydrogenase 1, mitochondrial
Gene
At1g53240, F12M16.14
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621NAD By similarity
Binding sitei109 – 1091Substrate By similarity
Binding sitei115 – 1151Substrate By similarity
Binding sitei122 – 1221NAD By similarity
Binding sitei147 – 1471Substrate By similarity
Binding sitei181 – 1811Substrate By similarity
Active sitei205 – 2051Proton acceptor By similarity
Binding sitei256 – 2561NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 427NAD By similarity
Nucleotide bindingi145 – 1473NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: TAIR
  2. copper ion binding Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. defense response to bacterium Source: TAIR
  3. malate metabolic process Source: InterPro
  4. response to cadmium ion Source: TAIR
  5. response to cold Source: TAIR
  6. response to salt stress Source: TAIR
  7. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT1G53240-MONOMER.
MetaCyc:AT1G53240-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase 1, mitochondrial (EC:1.1.1.37)
Alternative name(s):
mNAD-MDH 1
Gene namesi
Ordered Locus Names:At1g53240
ORF Names:F12M16.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G53240.

Subcellular locationi

Mitochondrion matrix 2 Publications

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. cell wall Source: TAIR
  3. chloroplast Source: TAIR
  4. mitochondrial matrix Source: UniProtKB-SubCell
  5. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222Mitochondrion1 Publication
Add
BLAST
Chaini23 – 341319Malate dehydrogenase 1, mitochondrial
PRO_0000018622Add
BLAST

Proteomic databases

PaxDbiQ9ZP06.
PRIDEiQ9ZP06.

2D gel databases

SWISS-2DPAGEQ9ZP06.

Expressioni

Gene expression databases

GenevestigatoriQ9ZP06.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

BioGridi26982. 1 interaction.
IntActiQ9ZP06. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9ZP06.
SMRiQ9ZP06. Positions 31-338.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
InParanoidiQ9ZP06.
KOiK00026.
OMAiYVQSTIT.
PhylomeDBiQ9ZP06.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZP06-1 [UniParc]FASTAAdd to Basket

« Hide

MFRSMLVRSS ASAKQAVIRR SFSSGSVPER KVAILGAAGG IGQPLALLMK    50
LNPLVSSLSL YDIANTPGVA ADVGHINTRS EVVGYMGDDN LAKALEGADL 100
VIIPAGVPRK PGMTRDDLFN INAGIVKNLC TAIAKYCPHA LINMISNPVN 150
STVPIAAEIF KKAGMYDEKK LFGVTTLDVV RARTFYAGKA NVPVAEVNVP 200
VIGGHAGVTI LPLFSQATPQ ANLSSDILTA LTKRTQDGGT EVVEAKAGKG 250
SATLSMAYAG ALFADACLKG LNGVPDVIEC SYVQSTITEL PFFASKVRLG 300
KNGVEEVLDL GPLSDFEKEG LEALKPELKS SIEKGVKFAN Q 341
Length:341
Mass (Da):35,804
Last modified:May 1, 1999 - v1
Checksum:iD035C4C38785BE57
GO

Sequence cautioni

The sequence AAF69549.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171V → A in AAM64855. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ131205 mRNA. Translation: CAA10320.1.
AC008007 Genomic DNA. Translation: AAF69549.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32910.1.
AF324670 mRNA. Translation: AAG40021.1.
AF339684 mRNA. Translation: AAK00366.1.
AY062580 mRNA. Translation: AAL32658.1.
AY128783 mRNA. Translation: AAM91183.1.
AY087304 mRNA. Translation: AAM64855.1.
PIRiT51311.
RefSeqiNP_564625.1. NM_104202.2.
UniGeneiAt.23771.

Genome annotation databases

EnsemblPlantsiAT1G53240.1; AT1G53240.1; AT1G53240.
GeneIDi841757.
KEGGiath:AT1G53240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ131205 mRNA. Translation: CAA10320.1 .
AC008007 Genomic DNA. Translation: AAF69549.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE32910.1 .
AF324670 mRNA. Translation: AAG40021.1 .
AF339684 mRNA. Translation: AAK00366.1 .
AY062580 mRNA. Translation: AAL32658.1 .
AY128783 mRNA. Translation: AAM91183.1 .
AY087304 mRNA. Translation: AAM64855.1 .
PIRi T51311.
RefSeqi NP_564625.1. NM_104202.2.
UniGenei At.23771.

3D structure databases

ProteinModelPortali Q9ZP06.
SMRi Q9ZP06. Positions 31-338.
ModBasei Search...

Protein-protein interaction databases

BioGridi 26982. 1 interaction.
IntActi Q9ZP06. 1 interaction.

2D gel databases

SWISS-2DPAGE Q9ZP06.

Proteomic databases

PaxDbi Q9ZP06.
PRIDEi Q9ZP06.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G53240.1 ; AT1G53240.1 ; AT1G53240 .
GeneIDi 841757.
KEGGi ath:AT1G53240.

Organism-specific databases

GeneFarmi 2023. 159.
TAIRi AT1G53240.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000213792.
InParanoidi Q9ZP06.
KOi K00026.
OMAi YVQSTIT.
PhylomeDBi Q9ZP06.

Enzyme and pathway databases

BioCyci ARA:AT1G53240-MONOMER.
MetaCyc:AT1G53240-MONOMER.

Gene expression databases

Genevestigatori Q9ZP06.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel, non-redox-regulated NAD-dependent malate dehydrogenase from chloroplasts of Arabidopsis thaliana L."
    Berkemeyer M., Scheibe R., Ocheretina O.
    J. Biol. Chem. 273:27927-27933(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Proteomic approach to identify novel mitochondrial proteins in Arabidopsis."
    Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.
    Plant Physiol. 127:1694-1710(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-37, SUBCELLULAR LOCATION.
    Tissue: Leaf and Stem.
  7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.
  8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMDHM1_ARATH
AccessioniPrimary (citable) accession number: Q9ZP06
Secondary accession number(s): Q8LBB9, Q9MAH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: June 11, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi