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Q9ZP05

- MDHG1_ARATH

UniProt

Q9ZP05 - MDHG1_ARATH

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Protein

Malate dehydrogenase, glyoxysomal

Gene

At5g09660

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751NADBy similarity
Binding sitei122 – 1221SubstratePROSITE-ProRule annotation
Binding sitei128 – 1281SubstratePROSITE-ProRule annotation
Binding sitei135 – 1351NADBy similarity
Binding sitei160 – 1601SubstratePROSITE-ProRule annotation
Binding sitei194 – 1941SubstratePROSITE-ProRule annotation
Active sitei218 – 2181Proton acceptorBy similarity
Binding sitei269 – 2691NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 557NADBy similarity
Nucleotide bindingi158 – 1603NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT5G09660-MONOMER.
ARA:GQT-2009-MONOMER.
ARA:GQT-2010-MONOMER.
ARA:GQT-2011-MONOMER.
MetaCyc:AT5G09660-MONOMER.
ReactomeiREACT_239253. Gluconeogenesis.
REACT_242439. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, glyoxysomal (EC:1.1.1.37)
Alternative name(s):
mbNAD-MDH
Gene namesi
Ordered Locus Names:At5g09660
ORF Names:F17I14_150, MTH16.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G09660.

Subcellular locationi

Glyoxysome Curated

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434GlyoxysomeSequence AnalysisAdd
BLAST
Chaini35 – 354320Malate dehydrogenase, glyoxysomalPRO_0000018634Add
BLAST

Proteomic databases

PaxDbiQ9ZP05.
PRIDEiQ9ZP05.

Expressioni

Gene expression databases

GenevestigatoriQ9ZP05.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi16103. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9ZP05.
SMRiQ9ZP05. Positions 42-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
InParanoidiQ9ZP05.
KOiK00026.
PhylomeDBiQ9ZP05.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9ZP05-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEFRGDANQR IARISAHLTP QMEAKNSVIG RENCRAKGGN PGFKVAILGA
60 70 80 90 100
AGGIGQSLSL LMKMNPLVSL LHLYDVVNAP GVTADVSHMD TGAVVRGFLG
110 120 130 140 150
AKQLEDALTG MDLVIIPAGI PRKPGMTRDD LFKINAGIVK TLCEGVAKCC
160 170 180 190 200
PNAIVNLISN PVNSTVPIAA EVFKKAGTYD PKKLLGVTTL DVARANTFVA
210 220 230 240 250
EVLGLDPREV DVPVVGGHAG VTILPLLSQV KPPSSFTPQE IEYLTNRIQN
260 270 280 290 300
GGTEVVEAKA GAGSATLSMA YAAAKFADAC LRGLRGDANV VECSFVASQV
310 320 330 340 350
TELAFFATKV RLGRTGAEEV YQLGPLNEYE RIGLEKAKDE LAGSIQKGVE

FIRK
Length:354
Mass (Da):37,369
Last modified:May 1, 1999 - v1
Checksum:iAC56EAC7AB025288
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ131206 mRNA. Translation: CAA10321.1.
AB020752 Genomic DNA. Translation: BAB09521.1.
AL353994 Genomic DNA. Translation: CAB89364.1.
CP002688 Genomic DNA. Translation: AED91422.1.
AF428373 mRNA. Translation: AAL16303.1.
AY037252 mRNA. Translation: AAK59853.1.
AY077653 mRNA. Translation: AAL76131.1.
PIRiT49932.
RefSeqiNP_196528.1. NM_121003.3. [Q9ZP05-1]
UniGeneiAt.40751.
At.47611.

Genome annotation databases

EnsemblPlantsiAT5G09660.1; AT5G09660.1; AT5G09660. [Q9ZP05-1]
GeneIDi830825.
KEGGiath:AT5G09660.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ131206 mRNA. Translation: CAA10321.1 .
AB020752 Genomic DNA. Translation: BAB09521.1 .
AL353994 Genomic DNA. Translation: CAB89364.1 .
CP002688 Genomic DNA. Translation: AED91422.1 .
AF428373 mRNA. Translation: AAL16303.1 .
AY037252 mRNA. Translation: AAK59853.1 .
AY077653 mRNA. Translation: AAL76131.1 .
PIRi T49932.
RefSeqi NP_196528.1. NM_121003.3. [Q9ZP05-1 ]
UniGenei At.40751.
At.47611.

3D structure databases

ProteinModelPortali Q9ZP05.
SMRi Q9ZP05. Positions 42-353.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 16103. 1 interaction.

Proteomic databases

PaxDbi Q9ZP05.
PRIDEi Q9ZP05.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G09660.1 ; AT5G09660.1 ; AT5G09660 . [Q9ZP05-1 ]
GeneIDi 830825.
KEGGi ath:AT5G09660.

Organism-specific databases

TAIRi AT5G09660.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000213792.
InParanoidi Q9ZP05.
KOi K00026.
PhylomeDBi Q9ZP05.

Enzyme and pathway databases

BioCyci ARA:AT5G09660-MONOMER.
ARA:GQT-2009-MONOMER.
ARA:GQT-2010-MONOMER.
ARA:GQT-2011-MONOMER.
MetaCyc:AT5G09660-MONOMER.
Reactomei REACT_239253. Gluconeogenesis.
REACT_242439. Citric acid cycle (TCA cycle).

Gene expression databases

Genevestigatori Q9ZP05.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel, non-redox-regulated NAD-dependent malate dehydrogenase from chloroplasts of Arabidopsis thaliana L."
    Berkemeyer M., Scheibe R., Ocheretina O.
    J. Biol. Chem. 273:27927-27933(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiMDHG1_ARATH
AccessioniPrimary (citable) accession number: Q9ZP05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: November 26, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3