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Protein

Adenylyltransferase and sulfurtransferase MOCS3

Gene

MOCS3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.UniRule annotation

Catalytic activityi

ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP.UniRule annotation
[Molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine + reduced acceptor = AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + [cysteine desulfurase] + oxidized acceptor.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis

This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101ATP; via amide nitrogenUniRule annotation1
Binding sitei122ATPUniRule annotation1
Binding sitei146ATPUniRule annotation1
Metal bindingi231ZincUniRule annotation1
Metal bindingi234ZincUniRule annotation1
Active sitei248Glycyl thioester intermediate; for adenylyltransferase activityUniRule annotation1
Metal bindingi306ZincUniRule annotation1
Metal bindingi309ZincUniRule annotation1
Active sitei422Cysteine persulfide intermediate; for sulfurtransferase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi129 – 133ATPUniRule annotation5
Nucleotide bindingi190 – 191ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Molybdenum cofactor biosynthesis, tRNA processing

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:GQT-2704-MONOMER.
ReactomeiR-ATH-947581. Molybdenum cofactor biosynthesis.
UniPathwayiUPA00344.
UPA00988.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyltransferase and sulfurtransferase MOCS3UniRule annotation
Alternative name(s):
Molybdenum cofactor synthesis protein 3UniRule annotation
Including the following 2 domains:
Molybdopterin-synthase adenylyltransferaseUniRule annotation (EC:2.7.7.80UniRule annotation)
Alternative name(s):
Adenylyltransferase MOCS3UniRule annotation
Sulfur carrier protein MOCS2A adenylyltransferaseUniRule annotation
Molybdopterin-synthase sulfurtransferaseUniRule annotation (EC:2.8.1.11UniRule annotation)
Alternative name(s):
Sulfur carrier protein MOCS2A sulfurtransferaseUniRule annotation
Sulfurtransferase 13UniRule annotation
Short name:
AtStr13
Sulfurtransferase MOCS3UniRule annotation
Gene namesi
Name:MOCS3UniRule annotation
Synonyms:CNX5UniRule annotation, STR13, UBA4UniRule annotation
Ordered Locus Names:At5g55130
ORF Names:MCO15.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G55130.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001205841 – 464Adenylyltransferase and sulfurtransferase MOCS3Add BLAST464

Proteomic databases

PaxDbiQ9ZNW0.

Expressioni

Gene expression databases

GenevisibleiQ9ZNW0. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G55130.1.

Structurei

3D structure databases

ProteinModelPortaliQ9ZNW0.
SMRiQ9ZNW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini358 – 462RhodaneseUniRule annotationAdd BLAST105

Sequence similaritiesi

In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.UniRule annotation
Contains 1 rhodanese domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG2017. Eukaryota.
COG0476. LUCA.
COG0607. LUCA.
HOGENOMiHOG000281219.
InParanoidiQ9ZNW0.
KOiK11996.
OMAiVRNPNEY.
OrthoDBiEOG093609IY.
PhylomeDBiQ9ZNW0.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_03049. MOCS3_Uba4. 1 hit.
InterProiIPR028885. MOCS3/Uba4.
IPR016040. NAD(P)-bd_dom.
IPR001763. Rhodanese-like_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ZNW0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMSNGGDSSE IVRELEELKL KKAEIEHRIS TLEAKLQDTA AVELYDAVSN
60 70 80 90 100
GDSYLTAPEL EHGLSPDQIY RYSRQLLLPS FAVEGQSNLL KSSVLVIGAG
110 120 130 140 150
GLGSPALLYL AACGVGQLGI IDHDVVELNN MHRQIIHTEA FIGHPKVKSA
160 170 180 190 200
AAACRSINST IKVDEYVEAL RTSNALEILS QYDIIVDATD NPPSRYMISD
210 220 230 240 250
CCVLLGKPLV SGAALGMEGQ LTVYNHNGGP CYRCLFPTPP PTSACQRCSD
260 270 280 290 300
SGVLGVVPGV IGCLQALETI KLASLVGEPL SERMLLFDAL SARMRIVKIR
310 320 330 340 350
GRSSQCTVCG DNSSFNKQTF KDFDYEDFTQ FPLFAGPLNL LPAESRISSK
360 370 380 390 400
EFKEILQKKE QHVLLDVRPS HHYKIVSLPD SLNIPLANLE TRLNELTSAL
410 420 430 440 450
KEKGNGHANT ESCTNPSVFV VCRRGNDSQR AVQYLRESGF DSAKDIIGGL
460
EAWAANVNPN FPTY
Length:464
Mass (Da):50,574
Last modified:May 1, 1999 - v1
Checksum:i844C7C35C7737940
GO
Isoform 2 (identifier: Q9ZNW0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     85-111: Missing.

Note: No experimental confirmation available.
Show »
Length:437
Mass (Da):47,993
Checksum:iD25E7E843FE21E11
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04263285 – 111Missing in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124160 Genomic DNA. Translation: AAD18051.1.
AF124159 mRNA. Translation: AAD18050.1.
AB010071 Genomic DNA. Translation: BAB08582.1.
CP002688 Genomic DNA. Translation: AED96586.1.
CP002688 Genomic DNA. Translation: AED96587.1.
BT029156 mRNA. Translation: ABJ17091.1.
AK317295 mRNA. Translation: BAH19971.1.
RefSeqiNP_001032076.1. NM_001036999.1. [Q9ZNW0-2]
NP_200324.1. NM_124895.3. [Q9ZNW0-1]
UniGeneiAt.24643.
At.48080.

Genome annotation databases

EnsemblPlantsiAT5G55130.1; AT5G55130.1; AT5G55130. [Q9ZNW0-1]
GeneIDi835604.
GrameneiAT5G55130.1; AT5G55130.1; AT5G55130.
KEGGiath:AT5G55130.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124160 Genomic DNA. Translation: AAD18051.1.
AF124159 mRNA. Translation: AAD18050.1.
AB010071 Genomic DNA. Translation: BAB08582.1.
CP002688 Genomic DNA. Translation: AED96586.1.
CP002688 Genomic DNA. Translation: AED96587.1.
BT029156 mRNA. Translation: ABJ17091.1.
AK317295 mRNA. Translation: BAH19971.1.
RefSeqiNP_001032076.1. NM_001036999.1. [Q9ZNW0-2]
NP_200324.1. NM_124895.3. [Q9ZNW0-1]
UniGeneiAt.24643.
At.48080.

3D structure databases

ProteinModelPortaliQ9ZNW0.
SMRiQ9ZNW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G55130.1.

Proteomic databases

PaxDbiQ9ZNW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G55130.1; AT5G55130.1; AT5G55130. [Q9ZNW0-1]
GeneIDi835604.
GrameneiAT5G55130.1; AT5G55130.1; AT5G55130.
KEGGiath:AT5G55130.

Organism-specific databases

TAIRiAT5G55130.

Phylogenomic databases

eggNOGiKOG2017. Eukaryota.
COG0476. LUCA.
COG0607. LUCA.
HOGENOMiHOG000281219.
InParanoidiQ9ZNW0.
KOiK11996.
OMAiVRNPNEY.
OrthoDBiEOG093609IY.
PhylomeDBiQ9ZNW0.

Enzyme and pathway databases

UniPathwayiUPA00344.
UPA00988.
BioCyciARA:GQT-2704-MONOMER.
ReactomeiR-ATH-947581. Molybdenum cofactor biosynthesis.

Miscellaneous databases

PROiQ9ZNW0.

Gene expression databases

GenevisibleiQ9ZNW0. AT.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_03049. MOCS3_Uba4. 1 hit.
InterProiIPR028885. MOCS3/Uba4.
IPR016040. NAD(P)-bd_dom.
IPR001763. Rhodanese-like_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOCS3_ARATH
AccessioniPrimary (citable) accession number: Q9ZNW0
Secondary accession number(s): B9DGV4, Q058R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.