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Q9ZNW0

- MOCS3_ARATH

UniProt

Q9ZNW0 - MOCS3_ARATH

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Protein
Adenylyltransferase and sulfurtransferase MOCS3
Gene
MOCS3, CNX5, STR13, UBA4, At5g55130, MCO15.8
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions By similarity.UniRule annotation

Catalytic activityi

ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP.UniRule annotation
[Molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine = AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + cysteine desulfurase.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011ATP; via amide nitrogen By similarity
Binding sitei122 – 1221ATP By similarity
Binding sitei146 – 1461ATP By similarity
Metal bindingi231 – 2311Zinc By similarity
Metal bindingi234 – 2341Zinc By similarity
Active sitei248 – 2481Glycyl thioester intermediate; for adenylyltransferase activity By similarity
Metal bindingi306 – 3061Zinc By similarity
Metal bindingi309 – 3091Zinc By similarity
Active sitei422 – 4221Cysteine persulfide intermediate; for sulfurtransferase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi129 – 1335ATP By similarity
Nucleotide bindingi190 – 1912ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. Mo-molybdopterin cofactor sulfurase activity Source: TAIR
  3. metal ion binding Source: UniProtKB-KW
  4. molybdopterin-synthase adenylyltransferase activity Source: UniProtKB-EC
  5. molybdopterin-synthase sulfurtransferase activity Source: UniProtKB-EC
  6. thiosulfate sulfurtransferase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. enzyme active site formation via L-cysteine persulfide Source: UniProtKB-HAMAP
  3. tRNA wobble position uridine thiolation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Molybdenum cofactor biosynthesis, tRNA processing

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:GQT-2702-MONOMER.
ARA:GQT-2704-MONOMER.
ReactomeiREACT_187830. Molybdenum cofactor biosynthesis.
UniPathwayiUPA00344.
UPA00988.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyltransferase and sulfurtransferase MOCS3
Alternative name(s):
Molybdenum cofactor synthesis protein 3
Including the following 2 domains:
Molybdopterin-synthase adenylyltransferase (EC:2.7.7.80)
Alternative name(s):
Adenylyltransferase MOCS3
Sulfur carrier protein MOCS2A adenylyltransferase
Molybdopterin-synthase sulfurtransferase (EC:2.8.1.11)
Alternative name(s):
Sulfur carrier protein MOCS2A sulfurtransferase
Sulfurtransferase 13
Short name:
AtStr13
Sulfurtransferase MOCS3
Gene namesi
Name:MOCS3
Synonyms:CNX5, STR13, UBA4
Ordered Locus Names:At5g55130
ORF Names:MCO15.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G55130.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Adenylyltransferase and sulfurtransferase MOCS3UniRule annotation
PRO_0000120584Add
BLAST

Proteomic databases

PaxDbiQ9ZNW0.
PRIDEiQ9ZNW0.

Expressioni

Gene expression databases

GenevestigatoriQ9ZNW0.

Structurei

3D structure databases

ProteinModelPortaliQ9ZNW0.
SMRiQ9ZNW0. Positions 28-310, 345-464.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini358 – 462105Rhodanese
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.
Contains 1 rhodanese domain.

Phylogenomic databases

eggNOGiCOG0476.
HOGENOMiHOG000281219.
InParanoidiQ9ZNW0.
KOiK11996.
OMAiVRKDPEC.
PhylomeDBiQ9ZNW0.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_03049. MOCS3_Uba4.
InterProiIPR028885. MOCS3/Uba4.
IPR007901. MoeZ_MoeB.
IPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR001763. Rhodanese-like_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF05237. MoeZ_MoeB. 1 hit.
PF00581. Rhodanese. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9ZNW0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMSNGGDSSE IVRELEELKL KKAEIEHRIS TLEAKLQDTA AVELYDAVSN    50
GDSYLTAPEL EHGLSPDQIY RYSRQLLLPS FAVEGQSNLL KSSVLVIGAG 100
GLGSPALLYL AACGVGQLGI IDHDVVELNN MHRQIIHTEA FIGHPKVKSA 150
AAACRSINST IKVDEYVEAL RTSNALEILS QYDIIVDATD NPPSRYMISD 200
CCVLLGKPLV SGAALGMEGQ LTVYNHNGGP CYRCLFPTPP PTSACQRCSD 250
SGVLGVVPGV IGCLQALETI KLASLVGEPL SERMLLFDAL SARMRIVKIR 300
GRSSQCTVCG DNSSFNKQTF KDFDYEDFTQ FPLFAGPLNL LPAESRISSK 350
EFKEILQKKE QHVLLDVRPS HHYKIVSLPD SLNIPLANLE TRLNELTSAL 400
KEKGNGHANT ESCTNPSVFV VCRRGNDSQR AVQYLRESGF DSAKDIIGGL 450
EAWAANVNPN FPTY 464
Length:464
Mass (Da):50,574
Last modified:May 1, 1999 - v1
Checksum:i844C7C35C7737940
GO
Isoform 2 (identifier: Q9ZNW0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     85-111: Missing.

Note: No experimental confirmation available.

Show »
Length:437
Mass (Da):47,993
Checksum:iD25E7E843FE21E11
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei85 – 11127Missing in isoform 2.
VSP_042632Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF124160 Genomic DNA. Translation: AAD18051.1.
AF124159 mRNA. Translation: AAD18050.1.
AB010071 Genomic DNA. Translation: BAB08582.1.
CP002688 Genomic DNA. Translation: AED96586.1.
CP002688 Genomic DNA. Translation: AED96587.1.
BT029156 mRNA. Translation: ABJ17091.1.
AK317295 mRNA. Translation: BAH19971.1.
RefSeqiNP_001032076.1. NM_001036999.1. [Q9ZNW0-2]
NP_200324.1. NM_124895.2. [Q9ZNW0-1]
UniGeneiAt.24643.
At.48080.

Genome annotation databases

EnsemblPlantsiAT5G55130.1; AT5G55130.1; AT5G55130. [Q9ZNW0-1]
GeneIDi835604.
KEGGiath:AT5G55130.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF124160 Genomic DNA. Translation: AAD18051.1 .
AF124159 mRNA. Translation: AAD18050.1 .
AB010071 Genomic DNA. Translation: BAB08582.1 .
CP002688 Genomic DNA. Translation: AED96586.1 .
CP002688 Genomic DNA. Translation: AED96587.1 .
BT029156 mRNA. Translation: ABJ17091.1 .
AK317295 mRNA. Translation: BAH19971.1 .
RefSeqi NP_001032076.1. NM_001036999.1. [Q9ZNW0-2 ]
NP_200324.1. NM_124895.2. [Q9ZNW0-1 ]
UniGenei At.24643.
At.48080.

3D structure databases

ProteinModelPortali Q9ZNW0.
SMRi Q9ZNW0. Positions 28-310, 345-464.
ModBasei Search...

Proteomic databases

PaxDbi Q9ZNW0.
PRIDEi Q9ZNW0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G55130.1 ; AT5G55130.1 ; AT5G55130 . [Q9ZNW0-1 ]
GeneIDi 835604.
KEGGi ath:AT5G55130.

Organism-specific databases

TAIRi AT5G55130.

Phylogenomic databases

eggNOGi COG0476.
HOGENOMi HOG000281219.
InParanoidi Q9ZNW0.
KOi K11996.
OMAi VRKDPEC.
PhylomeDBi Q9ZNW0.

Enzyme and pathway databases

UniPathwayi UPA00344 .
UPA00988 .
BioCyci ARA:GQT-2702-MONOMER.
ARA:GQT-2704-MONOMER.
Reactomei REACT_187830. Molybdenum cofactor biosynthesis.

Miscellaneous databases

PROi Q9ZNW0.

Gene expression databases

Genevestigatori Q9ZNW0.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_03049. MOCS3_Uba4.
InterProi IPR028885. MOCS3/Uba4.
IPR007901. MoeZ_MoeB.
IPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR001763. Rhodanese-like_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view ]
Pfami PF05237. MoeZ_MoeB. 1 hit.
PF00581. Rhodanese. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view ]
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF69572. SSF69572. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and sequencing of a genomic region coding for the MPT-synthase-sulphurylase (Cnx5) in A.thaliana."
    Nieder J., Gutzke G., Mendel R.R.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Molybdenum cofactor biosynthesis: identification of A.thaliana cDNAs homologous to the E.coli sulfotransferase MoeB."
    Nieder J., Stallmeyer B., Brinkmann H., Mendel R.R.
    (In) Cram W.J., de Kok L.J., Stulen I., Brunold C., Rennenberg H. (eds.); Sulphur Metabolism in Higher Plants, pp.275-277, Backhuys Publishers, Leiden (1997)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
    Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
    Tissue: Rosette leaf.
  7. "Differential expression of Arabidopsis sulfurtransferases under various growth conditions."
    Bartels A., Mock H.P., Papenbrock J.
    Plant Physiol. Biochem. 45:178-187(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.

Entry informationi

Entry nameiMOCS3_ARATH
AccessioniPrimary (citable) accession number: Q9ZNW0
Secondary accession number(s): B9DGV4, Q058R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: May 1, 1999
Last modified: September 3, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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