Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histidine-containing phosphotransfer protein 1

Gene

AHP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay.4 Publications

GO - Molecular functioni

  • histidine phosphotransfer kinase activity Source: TAIR
  • protein histidine kinase binding Source: UniProtKB

GO - Biological processi

  • cytokinin-activated signaling pathway Source: TAIR
  • phosphorelay signal transduction system Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Cytokinin signaling pathway, Stress response, Two-component regulatory system

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine-containing phosphotransfer protein 1
Gene namesi
Name:AHP1
Synonyms:ATHP3
Ordered Locus Names:At3g21510
ORF Names:MIL23.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G21510.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749271 – 154Histidine-containing phosphotransfer protein 1Add BLAST154

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei79PhosphohistidinePROSITE-ProRule annotation1

Post-translational modificationi

Two-component system major event consists of a His-to-Asp phosphorelay between a sensor histidine kinase (HK) and a response regulator (RR). In plants, the His-to-Asp phosphorelay involves an additional intermediate named Histidine-containing phosphotransfer protein (HPt). This multistep phosphorelay consists of a His-Asp-His-Asp sequential transfer of a phosphate group between first an His and an Asp of the HK protein, followed by the transfer to a conserved His of the HPt protein and finally the transfer to an Asp in the receiver domain of the RR protein.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9ZNV9.
PRIDEiQ9ZNV9.

Expressioni

Tissue specificityi

Strongly expressed in roots.2 Publications

Inductioni

By salt, cold and drought stress.1 Publication

Gene expression databases

GenevisibleiQ9ZNV9. AT.

Interactioni

Subunit structurei

Interacts with the B-type response regulators ARR1, ARR2, ARR4 and ARR9. Binds to ETR1, AHK2, AHK3, AHK4, AHK5 and FBR12.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AHK2Q9C5U22EBI-1100673,EBI-1100634
AHK3Q9C5U12EBI-1100673,EBI-1100653
ARR1Q940D03EBI-1100673,EBI-1100998
ARR14Q8L9Y32EBI-1100673,EBI-1100737
ARR2Q9ZWJ94EBI-1100673,EBI-1101028
ARR5Q9SB042EBI-1100673,EBI-1100883
ARR9O803663EBI-1100673,EBI-1100950
ETR1P493333EBI-1100673,EBI-1606682

GO - Molecular functioni

  • protein histidine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi7037. 28 interactors.
IntActiQ9ZNV9. 25 interactors.
STRINGi3702.AT3G21510.1.

Structurei

Secondary structure

1154
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 19Combined sources17
Helixi25 – 33Combined sources9
Helixi41 – 65Combined sources25
Beta strandi66 – 68Combined sources3
Helixi71 – 88Combined sources18
Helixi91 – 105Combined sources15
Helixi109 – 142Combined sources34

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EUKX-ray1.95B1-154[»]
ProteinModelPortaliQ9ZNV9.
SMRiQ9ZNV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 143HPtPROSITE-ProRule annotationAdd BLAST106

Domaini

Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.

Sequence similaritiesi

Contains 1 HPt domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4747. Eukaryota.
COG2198. LUCA.
HOGENOMiHOG000272680.
InParanoidiQ9ZNV9.
KOiK14490.
OMAiCEEQNTE.
OrthoDBiEOG09360S25.
PhylomeDBiQ9ZNV9.

Family and domain databases

Gene3Di1.20.120.160. 1 hit.
InterProiIPR008207. Sig_transdc_His_kin_Hpt_dom.
[Graphical view]
PfamiPF01627. Hpt. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
PROSITEiPS50894. HPT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZNV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLVQKQKSL QDYTKSLFLE GILDSQFLQL QQLQDESNPD FVSQVVTLFF
60 70 80 90 100
QDSDRILNDL SLSLDQQVVD FKKVDPHVHQ LKGSSSSIGA QRVKNACVVF
110 120 130 140 150
RSFCEQQNVE ACHRCLQQVK QEYYLVKNRL ETLFKLEQQI VASGGMIPAV

ELGF
Length:154
Mass (Da):17,615
Last modified:May 1, 1999 - v1
Checksum:iBA0C026EA19AF83D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012570 mRNA. Translation: BAA37112.1.
AB015141 mRNA. Translation: BAA36335.1.
AB019232 Genomic DNA. Translation: BAB02346.1.
CP002686 Genomic DNA. Translation: AEE76518.1.
AF370265 mRNA. Translation: AAK44080.1.
AY063082 mRNA. Translation: AAL34256.1.
RefSeqiNP_188788.1. NM_113046.4.
UniGeneiAt.24157.

Genome annotation databases

EnsemblPlantsiAT3G21510.1; AT3G21510.1; AT3G21510.
GeneIDi821705.
GrameneiAT3G21510.1; AT3G21510.1; AT3G21510.
KEGGiath:AT3G21510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012570 mRNA. Translation: BAA37112.1.
AB015141 mRNA. Translation: BAA36335.1.
AB019232 Genomic DNA. Translation: BAB02346.1.
CP002686 Genomic DNA. Translation: AEE76518.1.
AF370265 mRNA. Translation: AAK44080.1.
AY063082 mRNA. Translation: AAL34256.1.
RefSeqiNP_188788.1. NM_113046.4.
UniGeneiAt.24157.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EUKX-ray1.95B1-154[»]
ProteinModelPortaliQ9ZNV9.
SMRiQ9ZNV9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7037. 28 interactors.
IntActiQ9ZNV9. 25 interactors.
STRINGi3702.AT3G21510.1.

Proteomic databases

PaxDbiQ9ZNV9.
PRIDEiQ9ZNV9.

Protocols and materials databases

DNASUi821705.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G21510.1; AT3G21510.1; AT3G21510.
GeneIDi821705.
GrameneiAT3G21510.1; AT3G21510.1; AT3G21510.
KEGGiath:AT3G21510.

Organism-specific databases

TAIRiAT3G21510.

Phylogenomic databases

eggNOGiKOG4747. Eukaryota.
COG2198. LUCA.
HOGENOMiHOG000272680.
InParanoidiQ9ZNV9.
KOiK14490.
OMAiCEEQNTE.
OrthoDBiEOG09360S25.
PhylomeDBiQ9ZNV9.

Miscellaneous databases

PROiQ9ZNV9.

Gene expression databases

GenevisibleiQ9ZNV9. AT.

Family and domain databases

Gene3Di1.20.120.160. 1 hit.
InterProiIPR008207. Sig_transdc_His_kin_Hpt_dom.
[Graphical view]
PfamiPF01627. Hpt. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
PROSITEiPS50894. HPT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAHP1_ARATH
AccessioniPrimary (citable) accession number: Q9ZNV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.