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Protein

Histidine-containing phosphotransfer protein 2

Gene

AHP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay.4 Publications

GO - Molecular functioni

  • histidine phosphotransfer kinase activity Source: TAIR
  • protein histidine kinase binding Source: UniProtKB

GO - Biological processi

  • cytokinin-activated signaling pathway Source: TAIR
  • phosphorelay signal transduction system Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Cytokinin signaling pathway, Stress response, Two-component regulatory system

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine-containing phosphotransfer protein 2
Gene namesi
Name:AHP2
Synonyms:ATHP1
Ordered Locus Names:At3g29350
ORF Names:MUO10.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G29350.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: TAIR
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 156156Histidine-containing phosphotransfer protein 2PRO_0000074928Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei82 – 821PhosphohistidinePROSITE-ProRule annotation

Post-translational modificationi

Two-component system major event consists of a His-to-Asp phosphorelay between a sensor histidine kinase (HK) and a response regulator (RR). In plants, the His-to-Asp phosphorelay involves an additional intermediate named Histidine-containing phosphotransfer protein (HPt). This multistep phosphorelay consists of a His-Asp-His-Asp sequential transfer of a phosphate group between first an His and an Asp of the HK protein, followed by the transfer to a conserved His of the HPt protein and finally the transfer to an Asp in the receiver domain of the RR protein.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9ZNV8.
PRIDEiQ9ZNV8.

Expressioni

Tissue specificityi

Strongly expressed in flowers and roots. Detected also in leaves, siliques and stems.2 Publications

Inductioni

By salt, cold and drought stress.1 Publication

Gene expression databases

GenevisibleiQ9ZNV8. AT.

Interactioni

Subunit structurei

Interacts with the B-type response regulators ARR1, ARR2 and ARR10. Binds to AHK1, AHK2, AHK3, AHK4, AHK5, ETR1 and CKI1.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AHK2Q9C5U23EBI-1100687,EBI-1100634
AHK3Q9C5U12EBI-1100687,EBI-1100653
ARR14Q8L9Y33EBI-1100687,EBI-1100737
ARR16Q9SHC22EBI-1100687,EBI-1100982
ARR2Q9ZWJ94EBI-1100687,EBI-1101028
ARR7Q9ZWS73EBI-1100687,EBI-1100917
ARR9O803664EBI-1100687,EBI-1100950
DMC1Q390092EBI-1100687,EBI-307715
MND1Q8GYD22EBI-1100687,EBI-1554720

GO - Molecular functioni

  • protein histidine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi7922. 34 interactions.
IntActiQ9ZNV8. 29 interactions.
STRINGi3702.AT3G29350.1.

Structurei

Secondary structure

1
156
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 2120Combined sources
Helixi27 – 337Combined sources
Helixi43 – 6624Combined sources
Beta strandi69 – 713Combined sources
Helixi74 – 9118Combined sources
Helixi94 – 10815Combined sources
Helixi112 – 14433Combined sources
Turni145 – 1473Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4PACX-ray2.53A1-156[»]
ProteinModelPortaliQ9ZNV8.
SMRiQ9ZNV8. Positions 1-153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 147108HPtPROSITE-ProRule annotationAdd
BLAST

Domaini

Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.

Sequence similaritiesi

Contains 1 HPt domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4747. Eukaryota.
COG2198. LUCA.
HOGENOMiHOG000272680.
InParanoidiQ9ZNV8.
KOiK14490.
OMAiVDIEYKA.
PhylomeDBiQ9ZNV8.

Family and domain databases

Gene3Di1.20.120.160. 1 hit.
InterProiIPR008207. Sig_transdc_His_kin_Hpt_dom.
[Graphical view]
PfamiPF01627. Hpt. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
PROSITEiPS50894. HPT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ZNV8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDALIAQLQR QFRDYTISLY QQGFLDDQFT ELKKLQDDGS PDFVSEVLSL
60 70 80 90 100
FFEDCVKLIS NMARALDTTG TVDFSQVGAS VHQLKGSSSS VGAKRVKTLC
110 120 130 140 150
VSFKECCEAK NYEGCVRCLQ QVDIEYKALK TKLQDMFNLE KQIIQAGGIV

PQVDIN
Length:156
Mass (Da):17,476
Last modified:May 1, 1999 - v1
Checksum:i9A8FA1184F0E4462
GO
Isoform 2 (identifier: Q9ZNV8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-156: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.
Show »
Length:114
Mass (Da):12,689
Checksum:i0CF923F1EC5C16A9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei115 – 15642Missing in isoform 2. CuratedVSP_018143Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012568 mRNA. Translation: BAA37110.1.
AB015142 mRNA. Translation: BAA36336.1.
AP001309 Genomic DNA. Translation: BAB02580.1.
CP002686 Genomic DNA. Translation: AEE77571.1.
CP002686 Genomic DNA. Translation: AEE77572.1.
AY064148 mRNA. Translation: AAL36054.1.
AY097403 mRNA. Translation: AAM19919.1.
RefSeqiNP_189581.1. NM_113860.3. [Q9ZNV8-1]
NP_850649.1. NM_180318.2. [Q9ZNV8-2]
UniGeneiAt.22337.

Genome annotation databases

EnsemblPlantsiAT3G29350.1; AT3G29350.1; AT3G29350. [Q9ZNV8-1]
GeneIDi822593.
KEGGiath:AT3G29350.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012568 mRNA. Translation: BAA37110.1.
AB015142 mRNA. Translation: BAA36336.1.
AP001309 Genomic DNA. Translation: BAB02580.1.
CP002686 Genomic DNA. Translation: AEE77571.1.
CP002686 Genomic DNA. Translation: AEE77572.1.
AY064148 mRNA. Translation: AAL36054.1.
AY097403 mRNA. Translation: AAM19919.1.
RefSeqiNP_189581.1. NM_113860.3. [Q9ZNV8-1]
NP_850649.1. NM_180318.2. [Q9ZNV8-2]
UniGeneiAt.22337.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4PACX-ray2.53A1-156[»]
ProteinModelPortaliQ9ZNV8.
SMRiQ9ZNV8. Positions 1-153.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7922. 34 interactions.
IntActiQ9ZNV8. 29 interactions.
STRINGi3702.AT3G29350.1.

Proteomic databases

PaxDbiQ9ZNV8.
PRIDEiQ9ZNV8.

Protocols and materials databases

DNASUi822593.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G29350.1; AT3G29350.1; AT3G29350. [Q9ZNV8-1]
GeneIDi822593.
KEGGiath:AT3G29350.

Organism-specific databases

TAIRiAT3G29350.

Phylogenomic databases

eggNOGiKOG4747. Eukaryota.
COG2198. LUCA.
HOGENOMiHOG000272680.
InParanoidiQ9ZNV8.
KOiK14490.
OMAiVDIEYKA.
PhylomeDBiQ9ZNV8.

Miscellaneous databases

PROiQ9ZNV8.

Gene expression databases

GenevisibleiQ9ZNV8. AT.

Family and domain databases

Gene3Di1.20.120.160. 1 hit.
InterProiIPR008207. Sig_transdc_His_kin_Hpt_dom.
[Graphical view]
PfamiPF01627. Hpt. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
PROSITEiPS50894. HPT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of genes for two-component phosphorelay mediators with a single HPt domain in Arabidopsis thaliana."
    Miyata S., Urao T., Yamaguchi-Shinozaki K., Shinozaki K.
    FEBS Lett. 437:11-14(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Histidine-containing phosphotransfer (HPt) signal transducers implicated in His-to-Asp phosphorelay in Arabidopsis."
    Suzuki T., Imamura A., Ueguchi C., Mizuno T.
    Plant Cell Physiol. 39:1258-1268(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Possible His to Asp phosphorelay signaling in an Arabidopsis two-component system."
    Urao T., Miyata S., Yamaguchi-Shinozaki K., Shinozaki K.
    FEBS Lett. 478:227-232(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AHK1; ETR1 AND CKI1.
  7. "Two types of putative nuclear factors that physically interact with histidine-containing phosphotransfer (Hpt) domains, signaling mediators in His-to-Asp phosphorelay, in Arabidopsis thaliana."
    Suzuki T., Sakurai K., Ueguchi C., Mizuno T.
    Plant Cell Physiol. 42:37-45(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION.
  8. "The Arabidopsis sensor His-kinase, AHk4, can respond to cytokinins."
    Suzuki T., Miwa K., Ishikawa K., Yamada H., Aiba H., Mizuno T.
    Plant Cell Physiol. 42:107-113(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AHK4.
  9. "Two-component signal transduction pathways in Arabidopsis."
    Hwang I., Chen H.-C., Sheen J.
    Plant Physiol. 129:500-515(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
  10. "Comparative studies of the AHP histidine-containing phosphotransmitters implicated in His-to-Asp phosphorelay in Arabidopsis thaliana."
    Tanaka Y., Suzuki T., Yamashino T., Mizuno T.
    Biosci. Biotechnol. Biochem. 68:462-465(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION.
  11. "Analysis of protein interactions within the cytokinin-signaling pathway of Arabidopsis thaliana."
    Dortay H., Mehnert N., Buerkle L., Schmuelling T., Heyl A.
    FEBS J. 273:4631-4644(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AHK2; AHK3 AND AHK4.
  12. "Toward an interaction map of the two-component signaling pathway of Arabidopsis thaliana."
    Dortay H., Gruhn N., Pfeifer A., Schwerdtner M., Schmuelling T., Heyl A.
    J. Proteome Res. 7:3649-3660(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AHK2 AND AHK4, SUBCELLULAR LOCATION.
  13. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Structure-function analysis of Arabidopsis thaliana histidine kinase AHK5 bound to its cognate phosphotransfer protein AHP1."
    Bauer J., Reiss K., Veerabagu M., Heunemann M., Harter K., Stehle T.
    Mol. Plant 6:959-970(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AHK5.
  15. "Crystal structure of AHP2 from Arabidopsis thaliana."
    Degtjarik O., Dopitova R., Puehringer S., Reha D., Otrusinova O., Pekarova B., Kuty M., Zidek L., Janda L., Weiss M.S., Kuta-Smatanova I., Hejatko H.
    Submitted (APR-2014) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.53 ANGSTROMS).

Entry informationi

Entry nameiAHP2_ARATH
AccessioniPrimary (citable) accession number: Q9ZNV8
Secondary accession number(s): Q3EAX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: May 1, 1999
Last modified: July 6, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.