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Protein

Histidine-containing phosphotransfer protein 2

Gene

AHP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay.4 Publications

GO - Molecular functioni

  • histidine phosphotransfer kinase activity Source: TAIR
  • protein histidine kinase binding Source: UniProtKB

GO - Biological processi

  • cytokinin-activated signaling pathway Source: TAIR
  • phosphorelay signal transduction system Source: TAIR

Keywordsi

Biological processCytokinin signaling pathway, Stress response, Two-component regulatory system

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine-containing phosphotransfer protein 2
Gene namesi
Name:AHP2
Synonyms:ATHP1
Ordered Locus Names:At3g29350
ORF Names:MUO10.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G29350.
TAIRilocus:2093817. AT3G29350.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749281 – 156Histidine-containing phosphotransfer protein 2Add BLAST156

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei82PhosphohistidinePROSITE-ProRule annotation1

Post-translational modificationi

Two-component system major event consists of a His-to-Asp phosphorelay between a sensor histidine kinase (HK) and a response regulator (RR). In plants, the His-to-Asp phosphorelay involves an additional intermediate named Histidine-containing phosphotransfer protein (HPt). This multistep phosphorelay consists of a His-Asp-His-Asp sequential transfer of a phosphate group between first an His and an Asp of the HK protein, followed by the transfer to a conserved His of the HPt protein and finally the transfer to an Asp in the receiver domain of the RR protein.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9ZNV8.

PTM databases

iPTMnetiQ9ZNV8.

Expressioni

Tissue specificityi

Strongly expressed in flowers and roots. Detected also in leaves, siliques and stems.2 Publications

Inductioni

By salt, cold and drought stress.1 Publication

Gene expression databases

ExpressionAtlasiQ9ZNV8. baseline and differential.
GenevisibleiQ9ZNV8. AT.

Interactioni

Subunit structurei

Interacts with the B-type response regulators ARR1, ARR2 and ARR10. Binds to AHK1, AHK2, AHK3, AHK4, AHK5, ETR1 and CKI1.7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein histidine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi7922. 34 interactors.
IntActiQ9ZNV8. 29 interactors.
STRINGi3702.AT3G29350.1.

Structurei

Secondary structure

1156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 21Combined sources20
Helixi27 – 33Combined sources7
Helixi43 – 66Combined sources24
Beta strandi69 – 71Combined sources3
Helixi74 – 91Combined sources18
Helixi94 – 108Combined sources15
Helixi112 – 144Combined sources33
Turni145 – 147Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PACX-ray2.53A1-156[»]
ProteinModelPortaliQ9ZNV8.
SMRiQ9ZNV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 147HPtPROSITE-ProRule annotationAdd BLAST108

Domaini

Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.

Phylogenomic databases

eggNOGiKOG4747. Eukaryota.
COG2198. LUCA.
HOGENOMiHOG000272680.
InParanoidiQ9ZNV8.
KOiK14490.
OMAiIHDADNI.
OrthoDBiEOG09360S25.
PhylomeDBiQ9ZNV8.

Family and domain databases

CDDicd00088. HPT. 1 hit.
Gene3Di1.20.120.160. 1 hit.
InterProiView protein in InterPro
IPR036641. HPT_dom_sf.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
PfamiView protein in Pfam
PF01627. Hpt. 1 hit.
SUPFAMiSSF47226. SSF47226. 1 hit.
PROSITEiView protein in PROSITE
PS50894. HPT. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ZNV8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDALIAQLQR QFRDYTISLY QQGFLDDQFT ELKKLQDDGS PDFVSEVLSL
60 70 80 90 100
FFEDCVKLIS NMARALDTTG TVDFSQVGAS VHQLKGSSSS VGAKRVKTLC
110 120 130 140 150
VSFKECCEAK NYEGCVRCLQ QVDIEYKALK TKLQDMFNLE KQIIQAGGIV

PQVDIN
Length:156
Mass (Da):17,476
Last modified:May 1, 1999 - v1
Checksum:i9A8FA1184F0E4462
GO
Isoform 2 (identifier: Q9ZNV8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-156: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.
Show »
Length:114
Mass (Da):12,689
Checksum:i0CF923F1EC5C16A9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018143115 – 156Missing in isoform 2. CuratedAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012568 mRNA. Translation: BAA37110.1.
AB015142 mRNA. Translation: BAA36336.1.
AP001309 Genomic DNA. Translation: BAB02580.1.
CP002686 Genomic DNA. Translation: AEE77571.1.
CP002686 Genomic DNA. Translation: AEE77572.1.
AY064148 mRNA. Translation: AAL36054.1.
AY097403 mRNA. Translation: AAM19919.1.
RefSeqiNP_189581.1. NM_113860.4. [Q9ZNV8-1]
NP_850649.1. NM_180318.2. [Q9ZNV8-2]
UniGeneiAt.22337.

Genome annotation databases

EnsemblPlantsiAT3G29350.1; AT3G29350.1; AT3G29350. [Q9ZNV8-1]
AT3G29350.2; AT3G29350.2; AT3G29350. [Q9ZNV8-2]
GeneIDi822593.
GrameneiAT3G29350.1; AT3G29350.1; AT3G29350.
AT3G29350.2; AT3G29350.2; AT3G29350.
KEGGiath:AT3G29350.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAHP2_ARATH
AccessioniPrimary (citable) accession number: Q9ZNV8
Secondary accession number(s): Q3EAX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: May 1, 1999
Last modified: October 25, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references