Q9ZNT7 (PHB2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prohibitin-2, mitochondrial
Short name=Atphb2

Gene names

Name:	PHB2
Ordered Locus Names:	At1g03860
ORF Names:	F21M11.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins By similarity.
Subunit structure	Component of a prohibitin multimeric complex in mitochondrial membranes. Ref.6 Ref.10 Ref.11
Subcellular location	Mitochondrion inner membrane; Single-pass type II membrane protein Ref.6 Ref.7 Ref.10 Ref.11 Ref.12.
Tissue specificity	Mostly expressed in proliferative tissues, including vasculature, shoot and root apical tissues. Ref.10
Sequence similarities	Belongs to the prohibitin family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cell wall Inferred from direct assay PubMed 16287169. Source: TAIR chloroplast Inferred from direct assay PubMed 15028209. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex I Inferred from direct assay Ref.11PubMed 20197505. Source: TAIR vacuolar membrane Inferred from direct assay PubMed 17151019. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9ZNT7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9ZNT7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Prohibitin-2, mitochondrial		PRO_0000420597
Regions
	Topological domain	1 – 13	13	Mitochondrial matrix Potential
	Transmembrane	14 – 32	19	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	33 – 286	254	Mitochondrial intermembrane Potential
	Coiled coil	186 – 219	34	Potential
Natural variations
	Alternative sequence	1 – 65	65	Missing in isoform 2.		VSP_044541

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 89A48ECBA4F56DB8	FASTA	286	31,811
	10 20 30 40 50 60 MSFNKVPNIP GAPALSALLK VSVIGGLGVY ALTNSLYNVD GGHRAVMFNR LTGIKEKVYP 70 80 90 100 110 120 EGTHFMVPWF ERPIIYDVRA RPYLVESTTG SHDLQMVKIG LRVLTRPMGD RLPQIYRTLG 130 140 150 160 170 180 ENYSERVLPS IIHETLKAVV AQYNASQLIT QREAVSREIR KILTERASNF DIALDDVSIT 190 200 210 220 230 240 TLTFGKEFTA AIEAKQVAAQ EAERAKFIVE KAEQDRRSAV IRAQGEAKSA QLIGQAIANN 250 260 270 280 QAFITLRKIE AAREIAQTIA QSANKVYLSS NDLLLNLQEM NLEPKK
	Isoform 2 [UniParc]. Checksum: B5C238E9B8E9C162	FASTA	221	24,897
	10 20 30 40 50 60 MVPWFERPII YDVRARPYLV ESTTGSHDLQ MVKIGLRVLT RPMGDRLPQI YRTLGENYSE 70 80 90 100 110 120 RVLPSIIHET LKAVVAQYNA SQLITQREAV SREIRKILTE RASNFDIALD DVSITTLTFG 130 140 150 160 170 180 KEFTAAIEAK QVAAQEAERA KFIVEKAEQD RRSAVIRAQG EAKSAQLIGQ AIANNQAFIT 190 200 210 220 LRKIEAAREI AQTIAQSANK VYLSSNDLLL NLQEMNLEPK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis genes encoding prohibitin: importance for early development." Sun L., Goodman H.M. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: cv. Columbia.
[2]	"Cloning of an Arabidopsis thaliana prohibitin-like cDNA by functional complementation of a sulfate transport yeast mutant." Hatzfeld Y., Logan H.M., Cathala N., Davidian J.-C. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"Mitochondrial complex I from Arabidopsis and rice: orthologs of mammalian and fungal components coupled with plant-specific subunits." Heazlewood J.L., Howell K.A., Millar A.H. Biochim. Biophys. Acta 1604:159-169(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
[7]	"The hydrophobic proteome of mitochondrial membranes from Arabidopsis cell suspensions." Brugiere S., Kowalski S., Ferro M., Seigneurin-Berny D., Miras S., Salvi D., Ravanel S., d'Herin P., Garin J., Bourguignon J., Joyard J., Rolland N. Phytochemistry 65:1693-1707(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Strain: cv. Landsberg erecta.
[9]	"Mapping the Arabidopsis organelle proteome." Dunkley T.P.J., Hester S., Shadforth I.P., Runions J., Weimar T., Hanton S.L., Griffin J.L., Bessant C., Brandizzi F., Hawes C., Watson R.B., Dupree P., Lilley K.S. Proc. Natl. Acad. Sci. U.S.A. 103:6518-6523(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development." Van Aken O., Pecenkova T., van de Cotte B., De Rycke R., Eeckhout D., Fromm H., De Jaeger G., Witters E., Beemster G.T.S., Inze D., Van Breusegem F. Plant J. 52:850-864(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY. Strain: cv. Columbia.
[11]	"Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of gel electrophoresis." Meyer E.H., Taylor N.L., Millar A.H. J. Proteome Res. 7:786-794(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, SUBUNIT.
[12]	"Defining the protein complex proteome of plant mitochondria." Klodmann J., Senkler M., Rode C., Braun H.-P. Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U66592 mRNA. Translation: AAD00156.1. U89791 mRNA. Translation: AAD09244.1. AC003027 Genomic DNA. Translation: AAD10682.1. CP002684 Genomic DNA. Translation: AEE27624.1. CP002684 Genomic DNA. Translation: AEE27625.1. CP002684 Genomic DNA. Translation: AEE27626.1. AF370317 mRNA. Translation: AAK44132.1. AY063102 mRNA. Translation: AAL34276.1.
IPI	IPI00521125. IPI00531214.
PIR	C86169.
RefSeq	NP_171882.1. NM_100266.2. NP_973755.1. NM_202026.2. NP_973756.1. NM_202027.1.
UniGene	At.24021.
3D structure databases
ProteinModelPortal	Q9ZNT7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZNT7. 2 interactions.
Proteomic databases
PaxDb	Q9ZNT7.
PRIDE	Q9ZNT7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G03860.1; AT1G03860.1; AT1G03860. AT1G03860.3; AT1G03860.3; AT1G03860.
GeneID	839228.
KEGG	ath:AT1G03860.
Organism-specific databases
GeneFarm	4382. 443.
TAIR	At1g03860.
Phylogenomic databases
eggNOG	COG0330.
HOGENOM	HOG000205692.
InParanoid	Q9ZNT7.
OMA	HRAVMFN.
PhylomeDB	Q9ZNT7.
ProtClustDB	CLSN2679896.
Gene expression databases
ArrayExpress	Q9ZNT7.
Genevestigator	Q9ZNT7.
Family and domain databases
InterPro	IPR001107. Band_7. IPR000163. Prohibitin. [Graphical view]
PANTHER	PTHR23222. PTHR23222. 1 hit.
Pfam	PF01145. Band_7. 1 hit. [Graphical view]
PRINTS	PR00679. PROHIBITIN.
SMART	SM00244. PHB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHB2_ARATH

Accession

Primary (citable) accession number: Q9ZNT7
Secondary accession number(s): F4I2J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2013
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program