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Q9ZNT7 (PHB2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prohibitin-2, mitochondrial

Short name=Atphb2
Gene names
Name:PHB2
Ordered Locus Names:At1g03860
ORF Names:F21M11.21
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins By similarity.

Subunit structure

Component of a prohibitin multimeric complex in mitochondrial membranes. Ref.6 Ref.10 Ref.11

Subcellular location

Mitochondrion inner membrane; Single-pass type II membrane protein Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12.

Tissue specificity

Mostly expressed in proliferative tissues, including vasculature, shoot and root apical tissues. Ref.10

Sequence similarities

Belongs to the prohibitin family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9ZNT7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9ZNT7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-65: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 286285Prohibitin-2, mitochondrial
PRO_0000420597

Regions

Topological domain2 – 1312Mitochondrial matrix Potential
Transmembrane14 – 3219Helical; Signal-anchor for type II membrane protein; Potential
Topological domain33 – 286254Mitochondrial intermembrane Potential
Coiled coil186 – 21934 Potential

Natural variations

Alternative sequence2 – 6564Missing in isoform 2.
VSP_044541

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 89A48ECBA4F56DB8

FASTA28631,811
        10         20         30         40         50         60 
MSFNKVPNIP GAPALSALLK VSVIGGLGVY ALTNSLYNVD GGHRAVMFNR LTGIKEKVYP 

        70         80         90        100        110        120 
EGTHFMVPWF ERPIIYDVRA RPYLVESTTG SHDLQMVKIG LRVLTRPMGD RLPQIYRTLG 

       130        140        150        160        170        180 
ENYSERVLPS IIHETLKAVV AQYNASQLIT QREAVSREIR KILTERASNF DIALDDVSIT 

       190        200        210        220        230        240 
TLTFGKEFTA AIEAKQVAAQ EAERAKFIVE KAEQDRRSAV IRAQGEAKSA QLIGQAIANN 

       250        260        270        280 
QAFITLRKIE AAREIAQTIA QSANKVYLSS NDLLLNLQEM NLEPKK 

« Hide

Isoform 2 [UniParc].

Checksum: D9F26BEC88A9CCFB
Show »

FASTA22225,028

References

« Hide 'large scale' references
[1]"Arabidopsis genes encoding prohibitin: importance for early development."
Sun L., Goodman H.M.
Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[2]"Cloning of an Arabidopsis thaliana prohibitin-like cDNA by functional complementation of a sulfate transport yeast mutant."
Hatzfeld Y., Logan H.M., Cathala N., Davidian J.-C.
Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"Mitochondrial complex I from Arabidopsis and rice: orthologs of mammalian and fungal components coupled with plant-specific subunits."
Heazlewood J.L., Howell K.A., Millar A.H.
Biochim. Biophys. Acta 1604:159-169(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
[7]"The hydrophobic proteome of mitochondrial membranes from Arabidopsis cell suspensions."
Brugiere S., Kowalski S., Ferro M., Seigneurin-Berny D., Miras S., Salvi D., Ravanel S., d'Herin P., Garin J., Bourguignon J., Joyard J., Rolland N.
Phytochemistry 65:1693-1707(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Landsberg erecta.
[9]"Mapping the Arabidopsis organelle proteome."
Dunkley T.P.J., Hester S., Shadforth I.P., Runions J., Weimar T., Hanton S.L., Griffin J.L., Bessant C., Brandizzi F., Hawes C., Watson R.B., Dupree P., Lilley K.S.
Proc. Natl. Acad. Sci. U.S.A. 103:6518-6523(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development."
Van Aken O., Pecenkova T., van de Cotte B., De Rycke R., Eeckhout D., Fromm H., De Jaeger G., Witters E., Beemster G.T.S., Inze D., Van Breusegem F.
Plant J. 52:850-864(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: cv. Columbia.
[11]"Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of gel electrophoresis."
Meyer E.H., Taylor N.L., Millar A.H.
J. Proteome Res. 7:786-794(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, SUBUNIT.
[12]"Defining the protein complex proteome of plant mitochondria."
Klodmann J., Senkler M., Rode C., Braun H.-P.
Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U66592 mRNA. Translation: AAD00156.1.
U89791 mRNA. Translation: AAD09244.1.
AC003027 Genomic DNA. Translation: AAD10682.1.
CP002684 Genomic DNA. Translation: AEE27624.1.
CP002684 Genomic DNA. Translation: AEE27625.1.
CP002684 Genomic DNA. Translation: AEE27626.1.
AF370317 mRNA. Translation: AAK44132.1.
AY063102 mRNA. Translation: AAL34276.1.
PIRC86169.
RefSeqNP_171882.1. NM_100266.2. [Q9ZNT7-1]
NP_973755.1. NM_202026.2.
NP_973756.1. NM_202027.1. [Q9ZNT7-1]
UniGeneAt.24021.

3D structure databases

ProteinModelPortalQ9ZNT7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9ZNT7. 2 interactions.

Proteomic databases

PaxDbQ9ZNT7.
PRIDEQ9ZNT7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G03860.1; AT1G03860.1; AT1G03860. [Q9ZNT7-1]
AT1G03860.3; AT1G03860.3; AT1G03860. [Q9ZNT7-1]
GeneID839228.
KEGGath:AT1G03860.

Organism-specific databases

GeneFarm4382. 443.
TAIRAT1G03860.

Phylogenomic databases

eggNOGCOG0330.
HOGENOMHOG000205692.
InParanoidQ9ZNT7.
KOK17081.
OMAHRAVMFN.
PhylomeDBQ9ZNT7.

Gene expression databases

GenevestigatorQ9ZNT7.

Family and domain databases

InterProIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERPTHR23222. PTHR23222. 1 hit.
PfamPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSPR00679. PROHIBITIN.
SMARTSM00244. PHB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePHB2_ARATH
AccessionPrimary (citable) accession number: Q9ZNT7
Secondary accession number(s): F4I2J2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names