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Q9ZNT7

- PHB2_ARATH

UniProt

Q9ZNT7 - PHB2_ARATH

Protein

Prohibitin-2, mitochondrial

Gene

PHB2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins.By similarity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prohibitin-2, mitochondrial
    Short name:
    Atphb2
    Gene namesi
    Name:PHB2
    Ordered Locus Names:At1g03860
    ORF Names:F21M11.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G03860.

    Subcellular locationi

    Mitochondrion inner membrane 7 Publications; Single-pass type II membrane protein 7 Publications

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. chloroplast Source: TAIR
    3. integral component of membrane Source: UniProtKB-KW
    4. mitochondrial respiratory chain complex I Source: TAIR
    5. mitochondrion Source: TAIR
    6. plastid Source: TAIR
    7. vacuolar membrane Source: TAIR
    8. vacuole Source: TAIR

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 286285Prohibitin-2, mitochondrialPRO_0000420597Add
    BLAST

    Proteomic databases

    PaxDbiQ9ZNT7.
    PRIDEiQ9ZNT7.

    Expressioni

    Tissue specificityi

    Mostly expressed in proliferative tissues, including vasculature, shoot and root apical tissues.1 Publication

    Gene expression databases

    GenevestigatoriQ9ZNT7.

    Interactioni

    Subunit structurei

    Component of a prohibitin multimeric complex in mitochondrial membranes.3 Publications

    Protein-protein interaction databases

    IntActiQ9ZNT7. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZNT7.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1312Mitochondrial matrixSequence AnalysisAdd
    BLAST
    Topological domaini33 – 286254Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei14 – 3219Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili186 – 21934Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prohibitin family.Curated

    Keywords - Domaini

    Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0330.
    HOGENOMiHOG000205692.
    InParanoidiQ9ZNT7.
    KOiK17081.
    OMAiHRAVMFN.
    PhylomeDBiQ9ZNT7.

    Family and domain databases

    InterProiIPR001107. Band_7.
    IPR000163. Prohibitin.
    [Graphical view]
    PANTHERiPTHR23222. PTHR23222. 1 hit.
    PfamiPF01145. Band_7. 1 hit.
    [Graphical view]
    PRINTSiPR00679. PROHIBITIN.
    SMARTiSM00244. PHB. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9ZNT7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSFNKVPNIP GAPALSALLK VSVIGGLGVY ALTNSLYNVD GGHRAVMFNR    50
    LTGIKEKVYP EGTHFMVPWF ERPIIYDVRA RPYLVESTTG SHDLQMVKIG 100
    LRVLTRPMGD RLPQIYRTLG ENYSERVLPS IIHETLKAVV AQYNASQLIT 150
    QREAVSREIR KILTERASNF DIALDDVSIT TLTFGKEFTA AIEAKQVAAQ 200
    EAERAKFIVE KAEQDRRSAV IRAQGEAKSA QLIGQAIANN QAFITLRKIE 250
    AAREIAQTIA QSANKVYLSS NDLLLNLQEM NLEPKK 286
    Length:286
    Mass (Da):31,811
    Last modified:May 1, 1999 - v1
    Checksum:i89A48ECBA4F56DB8
    GO
    Isoform 2 (identifier: Q9ZNT7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-65: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:222
    Mass (Da):25,028
    Checksum:iD9F26BEC88A9CCFB
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2 – 6564Missing in isoform 2. CuratedVSP_044541Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U66592 mRNA. Translation: AAD00156.1.
    U89791 mRNA. Translation: AAD09244.1.
    AC003027 Genomic DNA. Translation: AAD10682.1.
    CP002684 Genomic DNA. Translation: AEE27624.1.
    CP002684 Genomic DNA. Translation: AEE27625.1.
    CP002684 Genomic DNA. Translation: AEE27626.1.
    AF370317 mRNA. Translation: AAK44132.1.
    AY063102 mRNA. Translation: AAL34276.1.
    PIRiC86169.
    RefSeqiNP_171882.1. NM_100266.2. [Q9ZNT7-1]
    NP_973755.1. NM_202026.2.
    NP_973756.1. NM_202027.1. [Q9ZNT7-1]
    UniGeneiAt.24021.

    Genome annotation databases

    EnsemblPlantsiAT1G03860.1; AT1G03860.1; AT1G03860. [Q9ZNT7-1]
    AT1G03860.3; AT1G03860.3; AT1G03860. [Q9ZNT7-1]
    GeneIDi839228.
    KEGGiath:AT1G03860.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U66592 mRNA. Translation: AAD00156.1 .
    U89791 mRNA. Translation: AAD09244.1 .
    AC003027 Genomic DNA. Translation: AAD10682.1 .
    CP002684 Genomic DNA. Translation: AEE27624.1 .
    CP002684 Genomic DNA. Translation: AEE27625.1 .
    CP002684 Genomic DNA. Translation: AEE27626.1 .
    AF370317 mRNA. Translation: AAK44132.1 .
    AY063102 mRNA. Translation: AAL34276.1 .
    PIRi C86169.
    RefSeqi NP_171882.1. NM_100266.2. [Q9ZNT7-1 ]
    NP_973755.1. NM_202026.2.
    NP_973756.1. NM_202027.1. [Q9ZNT7-1 ]
    UniGenei At.24021.

    3D structure databases

    ProteinModelPortali Q9ZNT7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9ZNT7. 3 interactions.

    Proteomic databases

    PaxDbi Q9ZNT7.
    PRIDEi Q9ZNT7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G03860.1 ; AT1G03860.1 ; AT1G03860 . [Q9ZNT7-1 ]
    AT1G03860.3 ; AT1G03860.3 ; AT1G03860 . [Q9ZNT7-1 ]
    GeneIDi 839228.
    KEGGi ath:AT1G03860.

    Organism-specific databases

    GeneFarmi 4382. 443.
    TAIRi AT1G03860.

    Phylogenomic databases

    eggNOGi COG0330.
    HOGENOMi HOG000205692.
    InParanoidi Q9ZNT7.
    KOi K17081.
    OMAi HRAVMFN.
    PhylomeDBi Q9ZNT7.

    Gene expression databases

    Genevestigatori Q9ZNT7.

    Family and domain databases

    InterProi IPR001107. Band_7.
    IPR000163. Prohibitin.
    [Graphical view ]
    PANTHERi PTHR23222. PTHR23222. 1 hit.
    Pfami PF01145. Band_7. 1 hit.
    [Graphical view ]
    PRINTSi PR00679. PROHIBITIN.
    SMARTi SM00244. PHB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis genes encoding prohibitin: importance for early development."
      Sun L., Goodman H.M.
      Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    2. "Cloning of an Arabidopsis thaliana prohibitin-like cDNA by functional complementation of a sulfate transport yeast mutant."
      Hatzfeld Y., Logan H.M., Cathala N., Davidian J.-C.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. "Mitochondrial complex I from Arabidopsis and rice: orthologs of mammalian and fungal components coupled with plant-specific subunits."
      Heazlewood J.L., Howell K.A., Millar A.H.
      Biochim. Biophys. Acta 1604:159-169(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. "Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development."
      Van Aken O., Pecenkova T., van de Cotte B., De Rycke R., Eeckhout D., Fromm H., De Jaeger G., Witters E., Beemster G.T.S., Inze D., Van Breusegem F.
      Plant J. 52:850-864(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: cv. Columbia.
    11. "Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of gel electrophoresis."
      Meyer E.H., Taylor N.L., Millar A.H.
      J. Proteome Res. 7:786-794(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, SUBUNIT.
    12. "Defining the protein complex proteome of plant mitochondria."
      Klodmann J., Senkler M., Rode C., Braun H.-P.
      Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPHB2_ARATH
    AccessioniPrimary (citable) accession number: Q9ZNT7
    Secondary accession number(s): F4I2J2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2013
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3