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Protein

NADH--cytochrome b5 reductase 1

Gene

CBR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reductase transferring electrons from NADH to cytochrome b5. Required for the NADH-dependent electron transfer involved in the desaturation and hydroxylation of fatty acids and in the desaturation of sterol precursors. No activity with NADPH as electron donor.2 Publications

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADBy similarity

Kineticsi

  1. KM=1.5 µM for NADH1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi129 – 144FADBy similarityAdd BLAST16
    Nucleotide bindingi155 – 187FADBy similarityAdd BLAST33

    GO - Molecular functioni

    • cytochrome-b5 reductase activity, acting on NAD(P)H Source: TAIR

    GO - Biological processi

    • electron transport chain Source: TAIR

    Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein, NAD

    Enzyme and pathway databases

    BioCyciARA:AT5G17770-MONOMER.
    ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH--cytochrome b5 reductase 1 (EC:1.6.2.2)
    Gene namesi
    Name:CBR1
    Synonyms:CBR
    Ordered Locus Names:At5g17770
    ORF Names:MVA3.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRilocus:2176001. AT5G17770.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei13 – 33HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    • endoplasmic reticulum Source: TAIR
    • integral component of membrane Source: UniProtKB-KW
    • mitochondrial outer membrane Source: UniProtKB-SubCell
    • plant-type cell wall Source: TAIR
    • plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi78L → F in cbr1-1; decreased stability and decreased activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004196221 – 281NADH--cytochrome b5 reductase 1Add BLAST281

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei166PhosphothreonineBy similarity1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9ZNT1.
    PRIDEiQ9ZNT1.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, flowers and siliques. Detected in leaves.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9ZNT1. baseline and differential.
    GenevisibleiQ9ZNT1. AT.

    Interactioni

    Subunit structurei

    Monomer. Interacts with AKR2A (PubMed:20215589).By similarity1 Publication

    Protein-protein interaction databases

    BioGridi16921. 2 interactors.
    STRINGi3702.AT5G17770.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZNT1.
    SMRiQ9ZNT1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini45 – 149FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST105

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi34 – 40AKR2A-binding sequence (ABS) required for mitochondrion outer membrane targeting1 Publication7

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0534. Eukaryota.
    COG0543. LUCA.
    HOGENOMiHOG000175005.
    InParanoidiQ9ZNT1.
    KOiK00326.
    OMAiPVWFLYN.
    OrthoDBiEOG09360I1T.
    PhylomeDBiQ9ZNT1.

    Family and domain databases

    InterProiView protein in InterPro
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR001834. NADH-Cyt_B5_reductase.
    IPR008333. OxRdtase_FAD-bd_dom.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    PfamiView protein in Pfam
    PF00970. FAD_binding_6. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PRINTSiPR00406. CYTB5RDTASE.
    PR00371. FPNCR.
    SUPFAMiSSF63380. SSF63380. 1 hit.
    PROSITEiView protein in PROSITE
    PS51384. FAD_FR. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9ZNT1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDTEFLRTLD RQILLGVFVA FVAVGAGAAY FLTSSKKRRV CLDPENFKEF
    60 70 80 90 100
    KLVKRHQLSH NVAKFVFELP TSTSVLGLPI GQHISCRGKD GQGEDVIKPY
    110 120 130 140 150
    TPTTLDSDVG RFELVIKMYP QGRMSHHFRE MRVGDHLAVK GPKGRFKYQP
    160 170 180 190 200
    GQFRAFGMLA GGSGITPMFQ VARAILENPT DKTKVHLIYA NVTYDDILLK
    210 220 230 240 250
    EELEGLTTNY PEQFKIFYVL NQPPEVWDGG VGFVSKEMIQ THCPAPASDI
    260 270 280
    QILRCGPPPM NKAMAANLEA LGYSPEMQFQ F
    Length:281
    Mass (Da):31,490
    Last modified:May 1, 1999 - v1
    Checksum:iE6654D95FEA2BCD2
    GO

    Sequence cautioni

    The sequence AAL24304 differs from that shown. Reason: Frameshift at position 39.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB007799 mRNA. Translation: BAA74837.1.
    AB007800 Genomic DNA. Translation: BAA74838.1.
    AB006706 Genomic DNA. Translation: BAB09576.1.
    CP002688 Genomic DNA. Translation: AED92466.1.
    AY059822 mRNA. Translation: AAL24304.1. Frameshift.
    AY072482 mRNA. Translation: AAL66897.1.
    AK316787 mRNA. Translation: BAH19505.1.
    AY085728 mRNA. Translation: AAM62946.1.
    PIRiT52470.
    RefSeqiNP_197279.1. NM_121783.5.
    UniGeneiAt.43640.

    Genome annotation databases

    EnsemblPlantsiAT5G17770.1; AT5G17770.1; AT5G17770.
    GeneIDi831645.
    GrameneiAT5G17770.1; AT5G17770.1; AT5G17770.
    KEGGiath:AT5G17770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB007799 mRNA. Translation: BAA74837.1.
    AB007800 Genomic DNA. Translation: BAA74838.1.
    AB006706 Genomic DNA. Translation: BAB09576.1.
    CP002688 Genomic DNA. Translation: AED92466.1.
    AY059822 mRNA. Translation: AAL24304.1. Frameshift.
    AY072482 mRNA. Translation: AAL66897.1.
    AK316787 mRNA. Translation: BAH19505.1.
    AY085728 mRNA. Translation: AAM62946.1.
    PIRiT52470.
    RefSeqiNP_197279.1. NM_121783.5.
    UniGeneiAt.43640.

    3D structure databases

    ProteinModelPortaliQ9ZNT1.
    SMRiQ9ZNT1.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi16921. 2 interactors.
    STRINGi3702.AT5G17770.1.

    Proteomic databases

    PaxDbiQ9ZNT1.
    PRIDEiQ9ZNT1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G17770.1; AT5G17770.1; AT5G17770.
    GeneIDi831645.
    GrameneiAT5G17770.1; AT5G17770.1; AT5G17770.
    KEGGiath:AT5G17770.

    Organism-specific databases

    AraportiAT5G17770.
    TAIRilocus:2176001. AT5G17770.

    Phylogenomic databases

    eggNOGiKOG0534. Eukaryota.
    COG0543. LUCA.
    HOGENOMiHOG000175005.
    InParanoidiQ9ZNT1.
    KOiK00326.
    OMAiPVWFLYN.
    OrthoDBiEOG09360I1T.
    PhylomeDBiQ9ZNT1.

    Enzyme and pathway databases

    BioCyciARA:AT5G17770-MONOMER.
    ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.

    Miscellaneous databases

    PROiPR:Q9ZNT1.

    Gene expression databases

    ExpressionAtlasiQ9ZNT1. baseline and differential.
    GenevisibleiQ9ZNT1. AT.

    Family and domain databases

    InterProiView protein in InterPro
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR001834. NADH-Cyt_B5_reductase.
    IPR008333. OxRdtase_FAD-bd_dom.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    PfamiView protein in Pfam
    PF00970. FAD_binding_6. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PRINTSiPR00406. CYTB5RDTASE.
    PR00371. FPNCR.
    SUPFAMiSSF63380. SSF63380. 1 hit.
    PROSITEiView protein in PROSITE
    PS51384. FAD_FR. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNB5R1_ARATH
    AccessioniPrimary (citable) accession number: Q9ZNT1
    Secondary accession number(s): Q93YQ9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2012
    Last sequence update: May 1, 1999
    Last modified: April 12, 2017
    This is version 118 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.