Q9ZNR6 (PDX12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pyridoxal biosynthesis protein PDX1.2
Short name=AtPDX1.2
Short name=AtPDX1;3

Gene names

Name:	PDX12
Synonyms:	A37, PDX1L2
Ordered Locus Names:	At3g16050
ORF Names:	MSL1.3

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the production of pyridoxal (vitamin B6) and indirect resistance to singlet oxygen-generating photosensitizers. Ref.6
Pathway	Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.
Subunit structure	Homodimer or heterodimer with PDX1.1 or PDX1.3. No interaction with PDX2.
Subcellular location	Cytoplasm Ref.6.
Tissue specificity	Expressed in callus tissues, flowers and roots. Weakly expressed in leaves and stems. Ref.1 Ref.7
Miscellaneous	Unlike PDX1.1 or PDX1.3, PDX1.2 is unable to interact with PDX2 and restore prototrophy in yeast snz1 mutants. In plants, synthesis of vitamin B6 does not involve deoxyxylulose 5-phosphate but utilizes intermediates from the pentose phosphate pathway and from glycolysis. Vitamin B6 is an essential quencher of singlet oxygen in plants, that can protect cellular membranes from lipid peroxidation.
Sequence similarities	Belongs to the pdxS/SNZ family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: InterPro pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay Ref.6. Source: TAIR
Molecular function	catalytic activity Inferred from electronic annotation. Source: InterPro protein heterodimerization activity Inferred from physical interaction Ref.7. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PDX13	Q8L940	5	EBI-1545987,EBI-1545956

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Probable pyridoxal biosynthesis protein PDX1.2		PRO_0000109367

Sequences

	Sequence		Length	Mass (Da)
	Q9ZNR6 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: D894BC393CBBBA6A	FASTA	314	33,836
	10 20 30 40 50 60 MADQAMTDQD QGAVTLYSGT AITDAKKNHP FSVKVGLAQV LRGGAIVEVS SVNQAKLAES 70 80 90 100 110 120 AGACSVIVSD PVRSRGGVRR MPDPVLIKEV KRAVSVPVMA RARVGHFVEA QILESLAVDY 130 140 150 160 170 180 IDESEIISVA DDDHFINKHN FRSPFICGCR DTGEALRRIR EGAAMIRIQG DLTATGNIAE 190 200 210 220 230 240 TVKNVRSLMG EVRVLNNMDD DEVFTFAKKI SAPYDLVAQT KQMGRVPVVQ FASGGITTPA 250 260 270 280 290 300 DAALMMQLGC DGVFVGSEVF DGPDPFKKLR SIVQAVQHYN DPHVLAEMSS GLENAMESLN 310 VRGDRIQDFG QGSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"T-DNA trapping of a cryptic promoter identifies an ortholog of highly conserved SNZ growth arrest response genes in Arabidopsis." Oekresz L., Mathe C., Horvath E., Schell J., Koncz C., Szabados L. Plant Sci. 138:217-228(1998) Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Vitamin B6 biosynthesis in higher plants." Tambasco-Studart M., Titiz O., Raschle T., Forster G., Amrhein N., Fitzpatrick T.B. Proc. Natl. Acad. Sci. U.S.A. 102:13687-13692(2005) [PubMed: 16157873] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Analysis of the Arabidopsis rsr4-1/pdx1-3 mutant reveals the critical function of the PDX1 protein family in metabolism, development, and vitamin B6 biosynthesis." Wagner S., Bernhardt A., Leuendorf J.E., Drewke C., Lytovchenko A., Mujahed N., Gurgui C., Frommer W.B., Leistner E., Fernie A.R., Hellmann H. Plant Cell 18:1722-1735(2006) [PubMed: 16766694] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH PDX1.1 AND PDX1.3. Strain: cv. C24.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF029980 mRNA. Translation: AAD01897.1. AF029981 Genomic DNA. Translation: AAD01898.1. AB012247 Genomic DNA. Translation: BAB02670.1. CP002686 Genomic DNA. Translation: AEE75766.1. BT003136 mRNA. Translation: AAO24568.1. AK228092 mRNA. Translation: BAF00051.1.
IPI	IPI00533004.
RefSeq	NP_188226.1. NM_112475.3.
UniGene	At.66514. At.7601.
3D structure databases
ProteinModelPortal	Q9ZNR6.
SMR	Q9ZNR6. Positions 27-293.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZNR6. 4 interactions.
STRING	Q9ZNR6.
Proteomic databases
PRIDE	Q9ZNR6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G16050.1; AT3G16050.1; AT3G16050.
GeneID	820850.
GenomeReviews	Gene locus AT3G16050 in contig BA000014_GR.
KEGG	ath:AT3G16050.
NMPDR	fig|3702.1.peg.13774.
Organism-specific databases
GeneFarm	3240.
TAIR	At3g16050.
Phylogenomic databases
eggNOG	KOG1606.
GeneTree	EPGT00050000010473.
HOGENOM	HBG292342.
InParanoid	Q9ZNR6.
OMA	RARVGHF.
PhylomeDB	Q9ZNR6.
ProtClustDB	CLSN2683112.
Gene expression databases
ArrayExpress	Q9ZNR6.
Genevestigator	Q9ZNR6.
GermOnline	AT3G16050. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR001852. Snz1p/Sor1. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 2 hits.
KO	K06215.
PANTHER	PTHR22854:SF9. PTHR22854:SF9. 1 hit.
Pfam	PF01680. SOR_SNZ. 1 hit. [Graphical view]
PIRSF	PIRSF029271. Pdx1. 1 hit.
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
PROSITE	PS01235. PDXS_SNZ_1. 1 hit. PS51129. PDXS_SNZ_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDX12_ARATH

Accession

Primary (citable) accession number: Q9ZNR6
Secondary accession number(s): Q0WS48

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	May 1, 1999
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program