Q9ZMW8 (LUXS_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-ribosylhomocysteine lyase
EC=4.4.1.21

Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS

Gene names

Name:	luxS
Ordered Locus Names:	jhp_0097

Organism

Helicobacter pylori (strain J99) (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD). HAMAP-Rule MF_00091
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP-Rule MF_00091
Cofactor	Binds 1 iron ion per subunit.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the LuxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular_function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 152

152

S-ribosylhomocysteine lyase HAMAP-Rule MF_00091

PRO_0000172230

Sites

Metal binding

55

1

Iron

Metal binding

59

1

Iron

Metal binding

122

1

Iron

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

152

Helix Strand Turn

Details...

Helix

6 – 9

4

Beta strand

17 – 27

11

Beta strand

33 – 40

8

Turn

44 – 46

3

Helix

51 – 68

18

Beta strand

72 – 77

6

Beta strand

81 – 90

10

Helix

94 – 108

15

Turn

119 – 121

3

Turn

123 – 126

4

Helix

130 – 142

13

Helix

144 – 146

3

Sequences

	Sequence		Length	Mass (Da)
	Q9ZMW8 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: B2E154CF36958315	FASTA	152	17,424
	10 20 30 40 50 60 MKMNVESFNL DHTKVKAPYV RIADRKKGVN GDLIVKYDVR FKQPNRDHMD MPSLHSLEHL 70 80 90 100 110 120 VAEIIRNHAN YVVDWSPMGC QTGFYLTVLN HDNYTEILEV LEKTMQDVLK AKEVPASNEK 130 140 150 QCGWAANHTL EGAQNLARAF LDKRAEWSEV GV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J99.
[2]	"A structural genomics approach to the study of quorum sensing: crystal structures of three LuxS orthologs." Lewis H.A., Furlong E.B., Laubert B., Eroshkina G.A., Batiyenko Y., Adams J.M., Bergseid M.G., Marsh C.D., Peat T.S., Sanderson W.E., Sauder J.M., Buchanan S.G. Structure 9:527-537(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE001439 Genomic DNA. Translation: AAD05688.1.

PIR

C71973.

RefSeq

NP_222818.1. NC_000921.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J6X	X-ray	2.38	A/B	1-152	[»]

ProteinModelPortal

Q9ZMW8.

SMR

Q9ZMW8. Positions 1-152.

ModBase

Search...

Protein-protein interaction databases

STRING

85963.jhp0097.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAD05688; AAD05688; jhp_0097.

GeneID

889399.

KEGG

hpj:jhp0097.

PATRIC

20604800. VBIHelPyl98156_0112.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG1854.

KO

K07173.

OMA

KAPYVRV.

ProtClustDB

PRK02260.

Enzyme and pathway databases

BioCyc

HPYL85963:GJB9-104-MONOMER.

Family and domain databases

Gene3D

3.30.1360.80. 1 hit.

HAMAP

MF_00091. LuxS.

InterPro

IPR011249. Metalloenz_LuxS/M16.
IPR003815. S-ribosylhomocysteinase.
[Graphical view]

Pfam

PF02664. LuxS. 1 hit.
[Graphical view]

PIRSF

PIRSF006160. AI2. 1 hit.

PRINTS

PR01487. LUXSPROTEIN.

ProDom

PD013172. S-ribosylhomocysteinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF63411. Metalloenz_metal-bd. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9ZMW8.

Entry information

Entry name

LUXS_HELPJ

Accession

Primary (citable) accession number: Q9ZMW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program