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Q9ZMW8

- LUXS_HELPJ

UniProt

Q9ZMW8 - LUXS_HELPJ

Protein

S-ribosylhomocysteine lyase

Gene

luxS

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).

    Catalytic activityi

    S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione.

    Cofactori

    Binds 1 iron ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi55 – 551Iron
    Metal bindingi59 – 591Iron
    Metal bindingi122 – 1221Iron

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. S-ribosylhomocysteine lyase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. quorum sensing Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Autoinducer synthesis, Quorum sensing

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciHPYL85963:GJB9-104-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-ribosylhomocysteine lyase (EC:4.4.1.21)
    Alternative name(s):
    AI-2 synthesis protein
    Autoinducer-2 production protein LuxS
    Gene namesi
    Name:luxS
    Ordered Locus Names:jhp_0097
    OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
    Taxonomic identifieri85963 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000804: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 152152S-ribosylhomocysteine lyasePRO_0000172230Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi85963.jhp0097.

    Structurei

    Secondary structure

    1
    152
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 94
    Beta strandi17 – 2711
    Beta strandi33 – 408
    Turni44 – 463
    Helixi51 – 6818
    Beta strandi72 – 776
    Beta strandi81 – 9010
    Helixi94 – 10815
    Turni119 – 1213
    Turni123 – 1264
    Helixi130 – 14213
    Helixi144 – 1463

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J6XX-ray2.38A/B1-152[»]
    ProteinModelPortaliQ9ZMW8.
    SMRiQ9ZMW8. Positions 1-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9ZMW8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LuxS family.Curated

    Phylogenomic databases

    eggNOGiCOG1854.
    KOiK07173.
    OMAiRDHLNSD.
    OrthoDBiEOG68WRBM.

    Family and domain databases

    Gene3Di3.30.1360.80. 1 hit.
    HAMAPiMF_00091. LuxS.
    InterProiIPR011249. Metalloenz_LuxS/M16.
    IPR003815. S-ribosylhomocysteinase.
    [Graphical view]
    PfamiPF02664. LuxS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006160. AI2. 1 hit.
    PRINTSiPR01487. LUXSPROTEIN.
    ProDomiPD013172. S-ribosylhomocysteinase. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF63411. SSF63411. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9ZMW8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMNVESFNL DHTKVKAPYV RIADRKKGVN GDLIVKYDVR FKQPNRDHMD    50
    MPSLHSLEHL VAEIIRNHAN YVVDWSPMGC QTGFYLTVLN HDNYTEILEV 100
    LEKTMQDVLK AKEVPASNEK QCGWAANHTL EGAQNLARAF LDKRAEWSEV 150
    GV 152
    Length:152
    Mass (Da):17,424
    Last modified:May 1, 1999 - v1
    Checksum:iB2E154CF36958315
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001439 Genomic DNA. Translation: AAD05688.1.
    PIRiC71973.
    RefSeqiNP_222818.1. NC_000921.1.
    WP_000783567.1. NC_000921.1.

    Genome annotation databases

    EnsemblBacteriaiAAD05688; AAD05688; jhp_0097.
    GeneIDi889399.
    KEGGihpj:jhp0097.
    PATRICi20604800. VBIHelPyl98156_0112.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001439 Genomic DNA. Translation: AAD05688.1 .
    PIRi C71973.
    RefSeqi NP_222818.1. NC_000921.1.
    WP_000783567.1. NC_000921.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1J6X X-ray 2.38 A/B 1-152 [» ]
    ProteinModelPortali Q9ZMW8.
    SMRi Q9ZMW8. Positions 1-152.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 85963.jhp0097.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD05688 ; AAD05688 ; jhp_0097 .
    GeneIDi 889399.
    KEGGi hpj:jhp0097.
    PATRICi 20604800. VBIHelPyl98156_0112.

    Phylogenomic databases

    eggNOGi COG1854.
    KOi K07173.
    OMAi RDHLNSD.
    OrthoDBi EOG68WRBM.

    Enzyme and pathway databases

    BioCyci HPYL85963:GJB9-104-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q9ZMW8.

    Family and domain databases

    Gene3Di 3.30.1360.80. 1 hit.
    HAMAPi MF_00091. LuxS.
    InterProi IPR011249. Metalloenz_LuxS/M16.
    IPR003815. S-ribosylhomocysteinase.
    [Graphical view ]
    Pfami PF02664. LuxS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006160. AI2. 1 hit.
    PRINTSi PR01487. LUXSPROTEIN.
    ProDomi PD013172. S-ribosylhomocysteinase. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF63411. SSF63411. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: J99 / ATCC 700824.
    2. "A structural genomics approach to the study of quorum sensing: crystal structures of three LuxS orthologs."
      Lewis H.A., Furlong E.B., Laubert B., Eroshkina G.A., Batiyenko Y., Adams J.M., Bergseid M.G., Marsh C.D., Peat T.S., Sanderson W.E., Sauder J.M., Buchanan S.G.
      Structure 9:527-537(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS).

    Entry informationi

    Entry nameiLUXS_HELPJ
    AccessioniPrimary (citable) accession number: Q9ZMW8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3