Q9ZMW7 (METB_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cystathionine gamma-synthase
Short name=CGS
EC=2.5.1.48

Alternative name(s):
O-succinylhomoserine (thiol)-lyase

Gene names

Name:	metB
Ordered Locus Names:	jhp_0098

Organism

Helicobacter pylori (strain J99) (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cystathionine gamma-synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Cystathionine gamma-synthase		PRO_0000114759
Amino acid modifications
	Modified residue	195	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZMW7 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 358BEAE6C5C802BA	FASTA	380	41,223
	10 20 30 40 50 60 MRMQTKLIHG GINEDATTGA VSVPIYQTST YRQDAIGRHK GYEYSRSGNP TRFALEELIA 70 80 90 100 110 120 DLEGGVKGFA FASGLAGIHA VFSLLQSGDH VLLGDDVYGG NFRLFNKVLV KNGLSCTIID 130 140 150 160 170 180 TSDLSQIKKA IKPNTKALYL ETPSNPLLKI TDLAQCASVA KEHNLLTIVD NTFATPYCQN 190 200 210 220 230 240 PLLLGTDIVA HNGTKYLGGH SDVVAGLVTT NNEALAQEFD FFQNAIGGVL GLQDSWLLQR 250 260 270 280 290 300 GIKTLGLRME AHQKNALCVA EFLEKHPKVE RVYYPGLPTH PNHELAKAQM RGFSGMFSFT 310 320 330 340 350 360 LKNDSEAVAF VESLKLFILG ESLGGVESLV GIPALMTHAC IPKAQREAAG IRDGLVRLSV 370 380 GIEHEQDLLE DLDQAFAKIS

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J99.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD05677.1.
PIR	D71973.
RefSeq	NP_222819.1. NC_000921.1.
3D structure databases
ProteinModelPortal	Q9ZMW7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZMW7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	889500.
GenomeReviews	Gene locus jhp_0098 in contig AE001439_GR.
KEGG	hpj:jhp0098.
NMPDR	fig|85963.1.peg.97.
PATRIC	20604802. VBIHelPyl98156_0113.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0626.
HOGENOM	HBG754729.
OMA	HENNAMA.
PhylomeDB	Q9ZMW7.
ProtClustDB	PRK06176.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0098-MONOMER.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K01760.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METB_HELPJ

Accession

Primary (citable) accession number: Q9ZMW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program