Reviewed, UniProtKB/Swiss-Prot Q9ZMW6 (CYSM_HELPJ)

Names and origin

Protein names

Recommended name:
    Cysteine synthase
      Short name=CSase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL

Gene names

Name:	cysM
Synonyms:	cysK
Ordered Locus Names:	jhp_0099

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Cysteine synthase		PRO_0000167111
Regions
	Region	179 – 183	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	75	1	Pyridoxal phosphate By similarity
	Binding site	266	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	45	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZMW6-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 4EED8C5ED0A6FF22	FASTA	305	33,127
	10 20 30 40 50 60 MILTAMQDAI GRTPIFKFTR KDYPIPLKSA IYAKLEHLNP GGSVKDRLGQ YLIKEAFRTR 70 80 90 100 110 120 KITSTTTIIE PTAGNTGIAL ALVAIKHHLK TIFVVPKKFS TEKQQIMRAL GALVINTPTS 130 140 150 160 170 180 EGISGAIKKS KELAESIPDS YLPLQFENPD NPAAYYHTLA PEIVQELGTN LTSFVAGIGS 190 200 210 220 230 240 GGTFAGMAKY LKERIPNIRL IGVEPEGSIL NGGEPGPHEI EGIGVEFIPP FFANLDIDRF 250 260 270 280 290 300 ETISDEEGFS YTRKLAKKNG LLVGSSSGAA FAAALKEVQR LPEGSQVLTI FPDMADRYLS KGIYS

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001439 Genomic DNA. Translation: AAD05678.1.
PIR	E71973.
RefSeq	NP_222820.1.
3D structure databases
HSSP	HSSP built from PDB template 1J6N based on UniProtKB Q9WZD3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZMW6.
Genome annotation databases
GeneID	889513.
GenomeReviews	Gene locus jhp_0099 in contig AE001439_GR.
KEGG	hpj:jhp0099.
NMPDR	fig|85963.1.peg.98.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZMW6.
OMA	LIKEAFR.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0099-MON.
BRENDA	2.5.1.47. 295085.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSM_HELPJ

Accession

Primary (citable) accession number: Q9ZMW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)