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Q9ZMV7

- TOP1_HELPJ

UniProt

Q9ZMV7 - TOP1_HELPJ

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Protein

DNA topoisomerase 1

Gene
topA, jhp_0108
Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium 1; catalytic By similarity
Sitei32 – 321Interaction with DNA By similarity
Metal bindingi82 – 821Magnesium 1; catalytic By similarity
Metal bindingi82 – 821Magnesium 2 By similarity
Metal bindingi84 – 841Magnesium 2 By similarity
Sitei139 – 1391Interaction with DNA By similarity
Sitei140 – 1401Interaction with DNA By similarity
Sitei143 – 1431Interaction with DNA By similarity
Active sitei297 – 2971O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei299 – 2991Interaction with DNA By similarity
Sitei484 – 4841Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri572 – 59827C4-type 1UniRule annotationAdd
BLAST
Zinc fingeri616 – 64227C4-type 2UniRule annotationAdd
BLAST
Zinc fingeri663 – 68927C4-type 3UniRule annotationAdd
BLAST
Zinc fingeri702 – 72524C4-type 4UniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciHPYL85963:GJB9-115-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:jhp_0108
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000804: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 736736DNA topoisomerase 1UniRule annotationPRO_0000145151Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi85963.jhp0108.

Structurei

3D structure databases

ProteinModelPortaliQ9ZMV7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 113112ToprimAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 1686Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri572 – 59827C4-type 1UniRule annotationAdd
BLAST
Zinc fingeri616 – 64227C4-type 2UniRule annotationAdd
BLAST
Zinc fingeri663 – 68927C4-type 3UniRule annotationAdd
BLAST
Zinc fingeri702 – 72524C4-type 4UniRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZMV7-1 [UniParc]FASTAAdd to Basket

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MKHLIIVESP AKAKTIKNFL DKNYEVVASK GHVRDLSKFA LGIKIDETGF    50
TPNYVVDKDH KELVKQIIEL SKKASITYIA TDEDREGEAI GYHVACLIGG 100
KLESYPRIVF HEITQNAILN VLKTPRKIDM FKVNAQQARR LLDRIVGFKL 150
SSLIASKITK GLSAGRVQSA ALKLVIDREK EIRPFKPLTY FTLDALFEPH 200
LEAQLISYKG NKLKAQELID EKKAQEIKNE LEKESYIISS IIKKSKKSPT 250
PPPFMTSTLQ QSASSLLGFS PTKTMSIAQK LYEGVATPQG VMGVITYMRT 300
DSLNIAKEAL EEARAKILKD YGKDYLPPKA KVYSSKNKNA QEAHEAIRPT 350
SIILEPNALK DYLKPEELKL YTLIYKRFLA SQMQDALFES QSVVVACEKG 400
EFKASGRKLL FDGHYKILGN DDKDKLLPNL KENDPIKLEK LESNAHVTEP 450
PARYSEASLI KVLESLGIGR PSTYAPTISL LQNRDYIKVE KKQISALESA 500
FKVIEILEKH FEEIVDSKFS ASLEEELDNI AQNKADYQQV LKDFYYPFMD 550
KIEAGKKNII SQKVHEKTGQ SCPKCGGELV KKNSRYGEFI ACNNYPKCKY 600
IKQTENANDE AKQELCEKCG GEMVQKFSRN GAFLACNNYP ECKNTKSLKN 650
TPNAKETIEG VKCPECGGDI ALKRSKKGSF YGCNNYPKCN FLSNHKPINK 700
RCEKCHYLMS ERIYRKKKAH ECIQCKERVF LEEDNG 736
Length:736
Mass (Da):83,263
Last modified:May 1, 1999 - v1
Checksum:i779EF5655F3C14CF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001439 Genomic DNA. Translation: AAD05695.1.
PIRiB71972.
RefSeqiNP_222829.1. NC_000921.1.

Genome annotation databases

EnsemblBacteriaiAAD05695; AAD05695; jhp_0108.
GeneIDi889464.
KEGGihpj:jhp0108.
PATRICi20604822. VBIHelPyl98156_0123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001439 Genomic DNA. Translation: AAD05695.1 .
PIRi B71972.
RefSeqi NP_222829.1. NC_000921.1.

3D structure databases

ProteinModelPortali Q9ZMV7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 85963.jhp0108.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD05695 ; AAD05695 ; jhp_0108 .
GeneIDi 889464.
KEGGi hpj:jhp0108.
PATRICi 20604822. VBIHelPyl98156_0123.

Phylogenomic databases

eggNOGi COG0551.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci HPYL85963:GJB9-115-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J99 / ATCC 700824.

Entry informationi

Entry nameiTOP1_HELPJ
AccessioniPrimary (citable) accession number: Q9ZMV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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