Reviewed, UniProtKB/Swiss-Prot Q9ZMV7 (TOP1_HELPJ)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	jhp_0108

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	DNA topoisomerase 1		PRO_0000145151
Regions
	Zinc finger	572 – 598	27	C4-type 1
	Zinc finger	616 – 642	27	C4-type 2
	Zinc finger	663 – 689	27	C4-type 3
	Zinc finger	702 – 725	24	C4-type 4
Sites
	Active site	297	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZMV7-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 779EF5655F3C14CF	FASTA	736	83,263
	10 20 30 40 50 60 MKHLIIVESP AKAKTIKNFL DKNYEVVASK GHVRDLSKFA LGIKIDETGF TPNYVVDKDH 70 80 90 100 110 120 KELVKQIIEL SKKASITYIA TDEDREGEAI GYHVACLIGG KLESYPRIVF HEITQNAILN 130 140 150 160 170 180 VLKTPRKIDM FKVNAQQARR LLDRIVGFKL SSLIASKITK GLSAGRVQSA ALKLVIDREK 190 200 210 220 230 240 EIRPFKPLTY FTLDALFEPH LEAQLISYKG NKLKAQELID EKKAQEIKNE LEKESYIISS 250 260 270 280 290 300 IIKKSKKSPT PPPFMTSTLQ QSASSLLGFS PTKTMSIAQK LYEGVATPQG VMGVITYMRT 310 320 330 340 350 360 DSLNIAKEAL EEARAKILKD YGKDYLPPKA KVYSSKNKNA QEAHEAIRPT SIILEPNALK 370 380 390 400 410 420 DYLKPEELKL YTLIYKRFLA SQMQDALFES QSVVVACEKG EFKASGRKLL FDGHYKILGN 430 440 450 460 470 480 DDKDKLLPNL KENDPIKLEK LESNAHVTEP PARYSEASLI KVLESLGIGR PSTYAPTISL 490 500 510 520 530 540 LQNRDYIKVE KKQISALESA FKVIEILEKH FEEIVDSKFS ASLEEELDNI AQNKADYQQV 550 560 570 580 590 600 LKDFYYPFMD KIEAGKKNII SQKVHEKTGQ SCPKCGGELV KKNSRYGEFI ACNNYPKCKY 610 620 630 640 650 660 IKQTENANDE AKQELCEKCG GEMVQKFSRN GAFLACNNYP ECKNTKSLKN TPNAKETIEG 670 680 690 700 710 720 VKCPECGGDI ALKRSKKGSF YGCNNYPKCN FLSNHKPINK RCEKCHYLMS ERIYRKKKAH 730 ECIQCKERVF LEEDNG

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001439 Genomic DNA. Translation: AAD05695.1.
PIR	B71972.
RefSeq	NP_222829.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZMV7.
Genome annotation databases
GeneID	889464.
GenomeReviews	Gene locus jhp_0108 in contig AE001439_GR.
KEGG	hpj:jhp0108.
NMPDR	fig|85963.1.peg.107.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZMV7.
OMA	AIAYHIA.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0108-MON.
BRENDA	5.99.1.2. 295085.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_HELPJ

Accession

Primary (citable) accession number: Q9ZMV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)