Q9ZMS0 (HCPD_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative beta-lactamase HcpD
EC=3.5.2.6

Alternative name(s):
Cysteine-rich protein D
Penicillin-binding protein 4
Short name=PBP 4

Gene names

Name:	hcpD
Ordered Locus Names:	jhp_0148

Organism

Helicobacter pylori (strain J99) (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May hydrolyze 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives. Binds to penicillin By similarity.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the hcp beta-lactamase family. Contains 4 TPR repeats.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Secreted
Domain	Repeat Signal TPR repeat
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 305	278	Putative beta-lactamase HcpD		PRO_0000013199
Regions
	Repeat	28 – 61	34	TPR 1
	Repeat	96 – 133	38	TPR 2
	Repeat	168 – 205	38	TPR 3
	Repeat	240 – 277	38	TPR 4
Amino acid modifications
	Disulfide bond	55 ↔ 63		Potential
	Disulfide bond	91 ↔ 99		Potential
	Disulfide bond	127 ↔ 135		Potential
	Disulfide bond	163 ↔ 171		Potential
	Disulfide bond	199 ↔ 207		Potential
	Disulfide bond	235 ↔ 243		Potential
	Disulfide bond	271 ↔ 279		Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9ZMS0 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 1B969C1EDAD5DEB2	FASTA	305	33,807
	10 20 30 40 50 60 MIKSWTKKWF LILFLMASCF GHLVATTGEK YFKMANQALK RGDYHRAVAF YKRSCNLRMG 70 80 90 100 110 120 VGCTSLGSMY EYGDGVDQNI SKAVFYYRRG CNLRNHLACA SLGSMYEDGD GVQKDFPKAI 130 140 150 160 170 180 YYYRRGCHLK GGVSCGSLGF MYFNGTGVKQ NYAKALSFSK YACSLNYGIS CNFVGYMYKS 190 200 210 220 230 240 AKGVEKDLKK ALANFKRGCH LKDGASCVSL GYLYEAGMDV KQNEEQALNL YKKGCSLKEG 250 260 270 280 290 300 SGCHNVAVMY YTGKGAPKDL EKATSYYKKG CALGFSGSCK VLEVIGKESD NLQDDAQNDT QDSVQ

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD05729.1.
PIR	F71968.
RefSeq	NP_222869.1. NC_000921.1.
3D structure databases
ProteinModelPortal	Q9ZMS0.
ModBase	Search...
Protein-protein interaction databases
STRING	85963.jhp0148.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD05729; AAD05729; jhp_0148.
GeneID	889026.
KEGG	hpj:jhp0148.
PATRIC	20604912. VBIHelPyl98156_0168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0790.
KO	K07126.
OMA	ISYYKKG.
ProtClustDB	CLSK495893.
Enzyme and pathway databases
BioCyc	HPYL85963:GJB9-155-MONOMER.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR006597. Sel1-like. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF08238. Sel1. 7 hits. [Graphical view]
SMART	SM00671. SEL1. 7 hits. [Graphical view]
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. Uncertain. PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCPD_HELPJ

Accession

Primary (citable) accession number: Q9ZMS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program