Reviewed, UniProtKB/Swiss-Prot Q9ZMP1 (FRDB_HELPJ)

Names and origin

Protein names

Recommended name:
    Fumarate reductase iron-sulfur subunit
    EC=1.3.99.1

Gene names

Name:	frdB
Ordered Locus Names:	jhp_0177

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Subunit structure	Part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur, and cytochrome b-556 By similarity.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Fumarate reductase iron-sulfur subunit		PRO_0000158704
Regions
	Domain	17 – 98	82	2Fe-2S ferredoxin-type
	Domain	145 – 174	30	4Fe-4S ferredoxin-type
Sites
	Metal binding	60	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	65	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	68	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	80	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	154	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	157	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	160	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	164	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	211	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	217	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	221	1	Iron-sulfur 2 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZMP1-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: D400D302E279DB4B	FASTA	245	27,625
	10 20 30 40 50 60 MSDNERTIVV RVLKFDPQSA VNKPHFKEYQ LKETPSMTLF IALNLIREHQ DPDLSFDFVC 70 80 90 100 110 120 RAGICGSCAM MVNGRPRLAC KTLTSSFENG VITLMPMPSF TLIKDLSVNT GDWFSDMTKR 130 140 150 160 170 180 VESWAHSKEE VDITKPEKRV EPDEAQEVFE LDRCIECGCC IASCGTKLMR PNFIGAAGMN 190 200 210 220 230 240 RAMRFMIDSH DERSDDDFYE LVGDDDGVFG CMSLIACHDT CPKELPLQSS IATLRNRMLK VGKSR

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD05761.1.
PIR	F71963.
RefSeq	NP_222898.1.
3D structure databases
SMR	Q9ZMP1. Positions 6-241.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZMP1. 1 interaction.
STRING	Q9ZMP1.
Genome annotation databases
GeneID	889211.
GenomeReviews	Gene locus jhp_0177 in contig AE001439_GR.
KEGG	hpj:jhp0177.
NMPDR	fig|85963.1.peg.176.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0479.
HOGENOM	HBG616382.
OMA	RVESWAH.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0177-MONOMER.
BRENDA	1.3.99.1. 295085.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. IPR004489. Succ_DH/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRDB_HELPJ

Accession

Primary (citable) accession number: Q9ZMP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)