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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygenBy similarity
Binding sitei92 – 921NAD; via carbonyl oxygenBy similarity
Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygenBy similarity
Active sitei145 – 1451Proton acceptorBy similarity
Active sitei155 – 1551Proton acceptorBy similarity
Binding sitei162 – 1621NADBy similarity
Sitei203 – 2031Involved in acyl-ACP bindingBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NADBy similarity
Nucleotide bindingi64 – 652NADBy similarity
Nucleotide bindingi191 – 1955NADBy similarity

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

GO - Biological processi

  1. fatty acid elongation Source: UniProtKB
  2. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciHPYL85963:GJB9-188-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:jhp_0181
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000804 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054903Add
BLAST

Proteomic databases

PRIDEiQ9ZMN7.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi85963.jhp0181.

Structurei

3D structure databases

ProteinModelPortaliQ9ZMN7.
SMRiQ9ZMN7. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiGILDMIH.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

Q9ZMN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLKGKKGL IVGVANNKSI AYGIAQSCFN QGATLAFTYL NESLEKRVRP
60 70 80 90 100
IAQELNSPYV YELDVSKEEH FKSLYNNIKQ DLGSLDFIVH SVAFAPKEAL
110 120 130 140 150
EGSLLETSKS AFNTAMEISV YSLIELTNTL KPLLNNGASV LTLSYLGSTK
160 170 180 190 200
YMAHYNVMGL AKAALESAVR YLAVDLGKHN IRVNALSAGP IRTLASSGIA
210 220 230 240 250
DFRMILKWNE INAPLRKNVS LEEVGNAGMY LLSSLSNGVS GEVHFVDAGY
260 270
HVMGMGAVEE KDNKATLLWD LHKEQ
Length:275
Mass (Da):30,026
Last modified:May 1, 1999 - v1
Checksum:iE44EA1A482A0341B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD05765.1.
PIRiB71964.
RefSeqiNP_222902.1. NC_000921.1.

Genome annotation databases

EnsemblBacteriaiAAD05765; AAD05765; jhp_0181.
KEGGihpj:jhp0181.
PATRICi20604984. VBIHelPyl98156_0204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD05765.1.
PIRiB71964.
RefSeqiNP_222902.1. NC_000921.1.

3D structure databases

ProteinModelPortaliQ9ZMN7.
SMRiQ9ZMN7. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85963.jhp0181.

Proteomic databases

PRIDEiQ9ZMN7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD05765; AAD05765; jhp_0181.
KEGGihpj:jhp0181.
PATRICi20604984. VBIHelPyl98156_0204.

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiGILDMIH.
OrthoDBiEOG6HF644.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciHPYL85963:GJB9-188-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J99 / ATCC 700824.

Entry informationi

Entry nameiFABI_HELPJ
AccessioniPrimary (citable) accession number: Q9ZMN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: April 1, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.