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Q9ZMN7 (FABI_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Short name=ENR
EC=1.3.1.9
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene names
Name:fabI
Ordered Locus Names:jhp_0181
OrganismHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99) [Complete proteome] [HAMAP]
Taxonomic identifier85963 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism By similarity.

Catalytic activity

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathway

Lipid metabolism; fatty acid biosynthesis.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
PRO_0000054903

Regions

Nucleotide binding19 – 202NAD By similarity
Nucleotide binding64 – 652NAD By similarity
Nucleotide binding191 – 1955NAD By similarity

Sites

Active site1451Proton acceptor By similarity
Active site1551Proton acceptor By similarity
Binding site131NAD; via carbonyl oxygen By similarity
Binding site921NAD; via carbonyl oxygen By similarity
Binding site951Substrate; via amide nitrogen and carbonyl oxygen By similarity
Binding site1621NAD By similarity
Site2031Involved in acyl-ACP binding By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZMN7 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: E44EA1A482A0341B

FASTA27530,026
        10         20         30         40         50         60 
MGFLKGKKGL IVGVANNKSI AYGIAQSCFN QGATLAFTYL NESLEKRVRP IAQELNSPYV 

        70         80         90        100        110        120 
YELDVSKEEH FKSLYNNIKQ DLGSLDFIVH SVAFAPKEAL EGSLLETSKS AFNTAMEISV 

       130        140        150        160        170        180 
YSLIELTNTL KPLLNNGASV LTLSYLGSTK YMAHYNVMGL AKAALESAVR YLAVDLGKHN 

       190        200        210        220        230        240 
IRVNALSAGP IRTLASSGIA DFRMILKWNE INAPLRKNVS LEEVGNAGMY LLSSLSNGVS 

       250        260        270 
GEVHFVDAGY HVMGMGAVEE KDNKATLLWD LHKEQ 

« Hide

References

[1]"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori."
Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. expand/collapse author list , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.
Nature 397:176-180(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J99 / ATCC 700824.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001439 Genomic DNA. Translation: AAD05765.1.
PIRB71964.
RefSeqNP_222902.1. NC_000921.1.

3D structure databases

ProteinModelPortalQ9ZMN7.
SMRQ9ZMN7. Positions 2-275.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85963.jhp0181.

Proteomic databases

PRIDEQ9ZMN7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD05765; AAD05765; jhp_0181.
GeneID889293.
KEGGhpj:jhp0181.
PATRIC20604984. VBIHelPyl98156_0204.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0623.
KOK00208.
OMAGILDMIH.
OrthoDBEOG6HF644.
ProtClustDBPRK08415.

Enzyme and pathway databases

BioCycHPYL85963:GJB9-188-MONOMER.
UniPathwayUPA00094.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSPR00081. GDHRDH.
ProtoNetSearch...

Entry information

Entry nameFABI_HELPJ
AccessionPrimary (citable) accession number: Q9ZMN7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: January 22, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways