Reviewed, UniProtKB/Swiss-Prot Q9ZMK8 (MSRAB_HELPJ)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase msrA/msrB
Including the following 2 domains:
    1- Recommended name:
            Peptide methionine sulfoxide reductase msrA
                Short name=Protein-methionine-S-oxide reductase
              EC=1.8.4.11
        Alternative name(s):
            Peptide-methionine (S)-S-oxide reductase
              Short name=Peptide Met(O) reductase
    2- Recommended name:
            Peptide methionine sulfoxide reductase msrB
              EC=1.8.4.12
        Alternative name(s):
            Peptide-methionine (R)-S-oxide reductase

Gene names

Name:	msrAB
Synonyms:	msrA
Ordered Locus Names:	jhp_0210

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine By similarity. HAMAP MF_01400
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01400 L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01400 Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin.
Sequence similarities	In the N-terminal section; belongs to the msrA Met sulfoxide reductase family. In the C-terminal section; belongs to the msrB Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein modification process Inferred from electronic annotation. Source: HAMAP
Molecular function	peptide-methionine (R)-S-oxide reductase activity Inferred from electronic annotation. Source: EC peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Peptide methionine sulfoxide reductase msrA/msrB HAMAP MF_01400		PRO_0000138514
Regions
	Region	36 – 189	154	Peptide methionine sulfoxide reductase A HAMAP MF_01400
	Region	206 – 329	124	Peptide methionine sulfoxide reductase B HAMAP MF_01400
Sites
	Active site	44	1	By similarity
	Active site	318	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZMK8-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6786490ABA0F2386	FASTA	359	41,249
	10 20 30 40 50 60 MKILSYLKKF YLFLLIGAIM QANESMGAKL PKTDERVIYL AGGCFWGLEA YMERIYGVID 70 80 90 100 110 120 ASSGYANGKT SSTNYEKLHE SDHAESVKVI YDPKKISLDK LLRYYFKVID PVSVNKQGND 130 140 150 160 170 180 VGRQYRTGIY YVNSADKEVI DNALKALQKE VKGKIAIEVE PLKNYVRAEE YHQDYLKKHP 190 200 210 220 230 240 GGYCHIDLKK ADEVIVDDDK YTKPSDEVLK KKLTKLQYEV TQNKHTEKPF ENEYYNKEEE 250 260 270 280 290 300 GIYVDITTGE PLFSSADKYD SGCGWPSFSK PINKDVVKYE DDESLNRKRI EVLSRIGKAH 310 320 330 340 350 LGHVFNDGPK ELGGLRYCIN SAALRFIPLK DMEKEGYGEF IPYIKKGELK KYIQDKKSH

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD05793.1.
PIR	E71960.
RefSeq	NP_222931.1.
3D structure databases
SMR	Q9ZMK8. Positions 35-196, 201-338.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZMK8.
Genome annotation databases
GeneID	889429.
GenomeReviews	Gene locus jhp_0210 in contig AE001439_GR.
KEGG	hpj:jhp0210.
NMPDR	fig|85963.1.peg.209.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0225.
HOGENOM	HBG715255.
OMA	DERVIYL.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0210-MONOMER.
BRENDA	1.8.4.11. 295085. 1.8.4.12. 295085.
Family and domain databases
HAMAP	MF_01400. MsrB. Fused. [Tree] MF_01401. MsrA. Fused. [Tree]
InterPro	IPR002579. Methionine_sulphoxide_MsrB. IPR011057. Mss4-like. IPR002569. Peptide_Met_Sox_Rdtase_MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit. G3DSA:2.170.150.20. MsrB. 1 hit.
Pfam	PF01625. PMSR. 1 hit. PF01641. SelR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00401. msrA. 1 hit. TIGR00357. MsrB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSRAB_HELPJ

Accession

Primary (citable) accession number: Q9ZMK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)