Q9ZMB0 (NADE_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	jhp_0312

Organism

Helicobacter pylori (strain J99) (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152174
Regions
	Nucleotide binding	31 – 38	8	ATP By similarity
Sites
	Active site	33	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZMB0 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: AFF42E5DCD93B932	FASTA	260	29,357
	10 20 30 40 50 60 MQKDYQKLIV YLCDFLEKEV QKKGFKKVVY GLSGGLDSAV VGVLCQKVFK ENAHALLMPS 70 80 90 100 110 120 SVSMPESRTD ALNLCEKFSI PYTEYSIAPY DKIFGSHFKD ASLTRKGNFC ARLRMAFLYD 130 140 150 160 170 180 YSLKSDSLVI GTSNKSERML GYGTLFGDLA CAINPIGELF KTEVYELAYY LNIPKKILNK 190 200 210 220 230 240 PPSADLFVGQ SDEKDLGYPY SVIDPLLKDI EALFQTKPIH LETLIQLGYA EILVKNIISR 250 260 IQKNAFKLEL PTIAKRFNPE

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J99.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD05885.1.
PIR	A71949.
RefSeq	NP_223031.1. NC_000921.1.
3D structure databases
ProteinModelPortal	Q9ZMB0.
SMR	Q9ZMB0. Positions 3-260.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZMB0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	889334.
GenomeReviews	Gene locus jhp_0312 in contig AE001439_GR.
KEGG	hpj:jhp0312.
NMPDR	fig|85963.1.peg.309.
PATRIC	20605276. VBIHelPyl98156_0347.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	KLIVYLC.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0312-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_HELPJ

Accession

Primary (citable) accession number: Q9ZMB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program