Reviewed, UniProtKB/Swiss-Prot Q9ZM19 (ISPDF_HELPJ)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	jhp_0404

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_0000075670
Regions
	Region	1 – 250	250	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	251 – 409	159	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	257	1	Divalent metal cation By similarity
	Metal binding	259	1	Divalent metal cation By similarity
	Metal binding	291	1	Divalent metal cation By similarity
	Site	51	1	Transition state stabilizer By similarity
	Site	58	1	Transition state stabilizer By similarity
	Site	178	1	Positions MEP for the nucleophilic attack By similarity
	Site	230	1	Positions MEP for the nucleophilic attack By similarity
	Site	283	1	Transition state stabilizer By similarity
	Site	382	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZM19-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 636B714E255DCF80	FASTA	409	45,706
	10 20 30 40 50 60 MSLIRVNGEA FKLSLESLEE DPFETKETLE TLETLIKQTS VVLLAAGESK RFSRAIKKQW 70 80 90 100 110 120 LRSHHTPLWL SVYESFKEAL DFKEVILVVS ELDYVYIQRH YPKIKLVKGG ASRQESVRNA 130 140 150 160 170 180 LKVIDSTYTI TSDVARGLAN MEALKSLFLT LQQTSHYCIA PYLPCYDTAI YYNEALDREA 190 200 210 220 230 240 IKLIQTPQLS HTKTLQSALN QGGFKDESSA ILQAFPNSVS YIEGSKDLHK LTTSGDLKFF 250 260 270 280 290 300 TPFFNPAKDT FIGMGFDTHA FIKDKPMVLG GVVLDCEFGL KAHSDGDALL HAVIDAILGA 310 320 330 340 350 360 IKGGDIGEWF PDNDPKYKNA SSKELLKIVL DFSQSIGFEL LEMGATIFSE IPKITPYKPA 370 380 390 400 ILENLSQLLG LEKSQISLKA TTMEKMGFIG KQEGLLVQAH VSMRYKQKL

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001439 Genomic DNA. Translation: AAD05981.1.
PIR	G71936.
RefSeq	NP_223123.1.
3D structure databases
HSSP	HSSP built from PDB template 1GX1 based on UniProtKB P36663.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZM19.
Genome annotation databases
GeneID	889224.
GenomeReviews	Gene locus jhp_0404 in contig AE001439_GR.
KEGG	hpj:jhp0404.
NMPDR	fig|85963.1.peg.401.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZM19.
OMA	GGDIGEW.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0404-MON.
BRENDA	2.7.7.60. 295085. 4.6.1.12. 295085.
Family and domain databases
HAMAP	MF_01520. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_HELPJ

Accession

Primary (citable) accession number: Q9ZM19

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)