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Protein

Beta sliding clamp

Gene

dnaN

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Beta sliding clamp
Short name:
Beta clamp
Short name:
Sliding clamp
Alternative name(s):
Beta-clamp processivity factor
DNA polymerase III beta sliding clamp subunit
DNA polymerase III subunit beta
Gene namesi
Name:dnaN
Ordered Locus Names:jhp_0452
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000804 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054391 – 374Beta sliding clampAdd BLAST374

Interactioni

Subunit structurei

Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.By similarity

Protein-protein interaction databases

STRINGi85963.jhp0452.

Structurei

3D structure databases

ProteinModelPortaliQ9ZLX4.
SMRiQ9ZLX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta sliding clamp family.Curated

Phylogenomic databases

eggNOGiENOG4105CZ8. Bacteria.
COG0592. LUCA.
KOiK02338.
OMAiNYEAVIP.
OrthoDBiPOG091H02B3.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiView protein in InterPro
IPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
PANTHERiPTHR30478:SF1. PTHR30478:SF1. 1 hit.
PfamiView protein in Pfam
PF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
SMARTiView protein in SMART
SM00480. POL3Bc. 1 hit.
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZLX4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKISVSKNDL ENTLRYLQAF LDKKDASSIA SHIHLEVIKE KLFLKASDSD
60 70 80 90 100
IGLKSYISTQ STDKEGVGTI NGKKFLDIIS CLKDSNIVLE TKDDSLVIKQ
110 120 130 140 150
NKSSFKLPMF DADEFPEFPV IDPKVSLEIN APFLVDAFKK IAPVIEQTSH
160 170 180 190 200
KRELAGVLMQ FNQKHQTLSV VGTDTKRLSY TQLEKISIHS TEEDISCILP
210 220 230 240 250
KRALLEILKL FYENFSFKSD GMLAVVENET HAFFTKLIDG NYPDYQKILP
260 270 280 290 300
KEYTSSFTLG KEEFKEGIKL CSSLSSTIKL TLEKNNALFE SLDSEHSETA
310 320 330 340 350
KTSVEIEKGL DIEKAFHLGV NAKFFLEALN ALGTTQFVLK CNEPSSPFLI
360 370
QEPLDEKQSH LNAKISTLMM PITL
Length:374
Mass (Da):42,047
Last modified:May 1, 1999 - v1
Checksum:iB0D55FE670AD55D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD06030.1.
PIRiA71931.
RefSeqiWP_000704288.1. NC_000921.1.

Genome annotation databases

EnsemblBacteriaiAAD06030; AAD06030; jhp_0452.
KEGGihpj:jhp_0452.

Similar proteinsi

Entry informationi

Entry nameiDPO3B_HELPJ
AccessioniPrimary (citable) accession number: Q9ZLX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: August 30, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families