Reviewed, UniProtKB/Swiss-Prot Q9ZLH0 (HEMN_HELPJ)

Names and origin

Protein names

Recommended name:
    Oxygen-independent coproporphyrinogen III oxidase
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.99.22

Gene names

Name:	hemN
Ordered Locus Names:	jhp_0610

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX By similarity.
Catalytic activity	Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO(2) + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen III (AdoMet route): step 1/1.
Subunit structure	Monomer.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen dehydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Oxygen-independent coproporphyrinogen III oxidase		PRO_0000109943
Regions
	Region	114 – 115	2	S-adenosyl-L-methionine 2 binding By similarity
Sites
	Metal binding	62	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	66	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	69	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Binding site	56	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	68	1	S-adenosyl-L-methionine 2; via carbonyl oxygen By similarity
	Binding site	113	1	S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	147	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	174	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	186	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	211	1	S-adenosyl-L-methionine 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZLH0-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 849CE88D15E1008B	FASTA	457	53,278
	10 20 30 40 50 60 MQTIDFEKFS QYSKPGPRYT SYPTAVEFKE NFNEESLKTA FFNHDNLKNP MPLSLYTHLP 70 80 90 100 110 120 FCRSACYFCA CSVIYTSLEE KKVRYISYLK KELALLKNAM DTNREVAQFH YGGGTPTFFS 130 140 150 160 170 180 PPQLDEITQS IQEVFPNFSQ DIEMSCEIDP RHFTKEHMQT LFDRGFNRLS FGVQDFDLEV 190 200 210 220 230 240 QKAIHRIQPF EMVQESVKLA RDYGIKSINF DLIYGLPNQT KEGFLKTLEW VLKLDPDRLA 250 260 270 280 290 300 VFNYAHVPWV KKTMRKIDET LLPSPRDKLE ILESLISFLE KANYQMIGMD HFAKSDNELY 310 320 330 340 350 360 LALQKAELRR NFQGYTTKKF TQTIGIGVTS IGEGSDYYTQ NYKDLHYYEK ALDLGHLPVE 370 380 390 400 410 420 RGVALSQEDV LRKEVIMQMM SNLKLDYSKI EEKFSIDFKA HFKKELEKLK PYEEAGLLSF 430 440 450 NSKGFEMTRT GGMLVRNMAM EFDAYLRGGE KHFSKTL

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001439 Genomic DNA. Translation: AAD06178.1.
PIR	H71911.
RefSeq	NP_223328.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	890302.
GenomeReviews	Gene locus jhp_0610 in contig AE001439_GR.
KEGG	hpj:jhp0610.
NMPDR	fig|85963.1.peg.606.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZLH0.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0610-MON.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR004558. HemN. IPR010723. HemN_C. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF06969. HemN_C. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00538. hemN. 1 hit.
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

HEMN_HELPJ

Accession

Primary (citable) accession number: Q9ZLH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	November 4, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)