Reviewed, UniProtKB/Swiss-Prot Q9ZLD9 (GYRA_HELPJ)

Names and originHide

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	jhp_0641

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributesHide

Sequence length	828 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

OntologiesHide

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 828	828	DNA gyrase subunit A		PRO_0000145237
Sites
	Active site	126	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

SequencesHide

	Sequence		Length	Mass (Da)
	Q9ZLD9-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: DE6E7DE4A7FBF150	FASTA	828	92,651
	10 20 30 40 50 60 MQDHLVNETK NIVEVGIDSS IEESYLAYSM SVIIGRALPD ARDGLKPVHR RILYAMHELG 70 80 90 100 110 120 LTSKVAYKKS ARIVGDVIGK YHPHGDTAVY DALVRMAQDF SMRLELVDGQ GNFGSIDGDN 130 140 150 160 170 180 AAAMRYTEAR MTKASEEILR DIDKDTIDFV PNYDDTLKEP DILPSRLPNL LVNGANGIAV 190 200 210 220 230 240 GMATSIPPHR IDEIIDALAH VLGNPNAELD KILEFVKGPD FPTGGIIYGK AGIVEAYKTG 250 260 270 280 290 300 RGRVKVRAKV HVEKTKNKEI IVLGEMPFQT NKAKLVEQIS DLAREKQIEG ISEVRDESDR 310 320 330 340 350 360 EGIRVVIELK RDAMSEIVLN HLYKLTTMET TFSIILLAIY NKEPKIFTLL ELLRLFLNHR 370 380 390 400 410 420 KTIIIRRTIF ELEKAKARAH ILEGYLIALD NIDEIVRLIK TSPSPEAAKN ALIERFSLSE 430 440 450 460 470 480 IQSKAILEMR LQRLTGLERD KIKEEYQNLL ELIDDLNGIL KSEDRLNEVV KTELLEVKEQ 490 500 510 520 530 540 FSSPRRTEIQ ESYESIDTED LIANEPMVVS MSYKGYVKRV DLKAYERQNR GGKGKLSGST 550 560 570 580 590 600 YEDDFIENFF VANTHDILLF ITNKGQLYHL KVYKIPEASR IAMGKAIVNL ISLAPNEKIM 610 620 630 640 650 660 ATLSTKDFSD ERSLAFFTKN GVVKRTNLSE FGGNRSYSGI RAIVLDEGDE LVGAKVVDKN 670 680 690 700 710 720 AKHLLIASYL GMFIKFPLED VREIGRTTRG VMGIRLNEND FVVGAVVISD DSNKLLSVSE 730 740 750 760 770 780 NGLGKQTLAE AYREQSRGGK GVIGMKLTQK TGNLVSVISV DDENLNLMIL TASAKMIRVS 790 800 810 820 IKDIRETGRN ASGVKLINTA DKVVYVNSCP KEEEPENLET SSVQNLFE

ReferencesHide

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

+

Additional computationally mapped references.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD06219.1.
PIR	E71906.
RefSeq	NP_223359.1.
3D structure databases
SMR	Q9ZLD9. Positions 34-490, 504-806.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ZLD9. 1 interaction.
STRING	Q9ZLD9.
Genome annotation databases
GeneID	890268.
GenomeReviews	Gene locus jhp_0641 in contig AE001439_GR.
KEGG	hpj:jhp0641.
NMPDR	fig|85963.1.peg.637.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0188.
HOGENOM	HBG635183.
OMA	NTHDILL.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0641-MONOMER.
BRENDA	5.99.1.3. 295085.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry informationHide

Entry name

GYRA_HELPJ

Accession

Primary (citable) accession number: Q9ZLD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	March 2, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)