Reviewed, UniProtKB/Swiss-Prot Q9ZLB9 (ASPG_HELPJ)

Names and origin

Protein names

Recommended name:
    Probable L-asparaginase
      Short name=L-ASNase
    EC=3.5.1.1
Alternative name(s):
    L-asparagine amidohydrolase

Gene names

Name:	ansA
Synonyms:	asn
Ordered Locus Names:	jhp_0661

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the asparaginase 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Probable L-asparaginase		PRO_0000171082
Sites
	Active site	16	1	By similarity
	Active site	95	1	By similarity
	Active site	96	1	By similarity
	Active site	168	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZLB9-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 1C6FB0C7D4FC3095	FASTA	332	35,687
	10 20 30 40 50 60 MAQNLPTIAL LATGGTIAGS GVDASLGSYK SGELGVKELL KAIPSLNKIA RIQGEQVSNI 70 80 90 100 110 120 GSQDMNEEIW FKLAQRAQEL LDDSRIQGVV ITHGTDTLEE SAYFLNLVLH STKPVVLVGA 130 140 150 160 170 180 MRNASSLSAD GALNLYYAVS VAVNEKSANK GVLVVMDDTI FRVREVVKTH TTHISTFKAL 190 200 210 220 230 240 NSGAIGSVYY GKTRYYMQPL RKHTTESEFS LSQLKTPLPK VDIIYTHAGM TPDLFQASLN 250 260 270 280 290 300 SHAKGVVIAG VGNGNVSAGF LKAMQEASQM GVVIVRSSRV GSGGVTSGEI DDKAYGFITS 310 320 330 DNLNPQKARV LLQLALTKTN DKAKIQEMFE EY

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001439 Genomic DNA. Translation: AAD06238.1.
PIR	D71904.
RefSeq	NP_223379.1.
3D structure databases
HSSP	HSSP built from PDB template 1HG1 based on UniProtKB P06608.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZLB9.
Genome annotation databases
GeneID	890242.
GenomeReviews	Gene locus jhp_0661 in contig AE001439_GR.
KEGG	hpj:jhp0661.
NMPDR	fig|85963.1.peg.657.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZLB9.
OMA	NFGPLGY.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0661-MON.
BRENDA	3.5.1.1. 295085.
Family and domain databases
InterPro	IPR004550. AsnASE_II. IPR006034. Asp/Glutamnse. [Graphical view]
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PRINTS	PR00139. ASNGLNASE.
ProDom	PD003221. Asp/Glutamnse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00520. asnASE_II. 1 hit.
PROSITE	PS00144. ASN_GLN_ASE_1. 1 hit. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPG_HELPJ

Accession

Primary (citable) accession number: Q9ZLB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)