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Q9ZL96 (TRMB_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:jhp_0684
OrganismHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99) [Complete proteome] [HAMAP]
Taxonomic identifier85963 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171336

Sites

Binding site1241S-adenosyl-L-methionine By similarity
Binding site1491S-adenosyl-L-methionine By similarity
Binding site1761S-adenosyl-L-methionine By similarity
Binding site2321Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZL96 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 8B92B929183D3573

FASTA40046,403
        10         20         30         40         50         60 
MPHFLAKLDS KPLEYPLIRG DFCFHREFLS LKHPTKSCVH ASFKNDVFLL QKIRRAGDFL 

        70         80         90        100        110        120 
IKSEKATPLK REILKQALRI YSQSFEVISH NLQENSKHAS QKKALDLETF EDFIQKNQAP 

       130        140        150        160        170        180 
ILIEIGFGSG RHLIELAKNN PTTTCLGIEI HTPSIAQALK QIELLDLKNL HILQGDGRLV 

       190        200        210        220        230        240 
LESMPNHRCE KIFVHFPVPW NEKKHRRVLS EKFLNEALRV LKPRGFLELR TDDSLCFEDS 

       250        260        270        280        290        300 
LKLALKNFQC EIEIKKNAQI PVVSKYEARW NKLKKDIYDL KIYSLGLDEN PTQNHALDFS 

       310        320        330        340        350        360 
FDTITIDKES VGAILKTPKI IKEGYFVHVC NIYENKGDFL VELSMGDFDW PVRLFVLLAE 

       370        380        390        400 
NQVFYLNKSP LKTLNNHKAH LLLQNILSQK ELDERDHRSE 

« Hide

References

[1]"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori."
Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. expand/collapse author list , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.
Nature 397:176-180(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J99 / ATCC 700824.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001439 Genomic DNA. Translation: AAD06272.1.
PIRD71900.
RefSeqNP_223402.1. NC_000921.1.

3D structure databases

ProteinModelPortalQ9ZL96.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85963.jhp0684.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD06272; AAD06272; jhp_0684.
GeneID890215.
KEGGhpj:jhp0684.
PATRIC20606087. VBIHelPyl98156_0749.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMASKYEARW.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycHPYL85963:GJB9-696-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_HELPJ
AccessionPrimary (citable) accession number: Q9ZL96
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 1999
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways