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Protein

Cyclic pyranopterin monophosphate synthase

Gene

moaA

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).UniRule annotation

Catalytic activityi

GTP = cyclic pyranopterin phosphate + diphosphate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation
  • S-adenosyl-L-methionineUniRule annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141GTPUniRule annotation
Metal bindingi21 – 211Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi25 – 251Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Binding sitei27 – 271S-adenosyl-L-methionineUniRule annotation
Metal bindingi28 – 281Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Binding sitei64 – 641GTPUniRule annotation
Binding sitei68 – 681S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei95 – 951GTPUniRule annotation
Binding sitei119 – 1191S-adenosyl-L-methionineUniRule annotation
Binding sitei155 – 1551GTPUniRule annotation
Binding sitei189 – 1891S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi249 – 2491Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation
Metal bindingi252 – 2521Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation
Metal bindingi266 – 2661Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. cyclic pyranopterin monophosphate synthase activity Source: UniProtKB-EC
  3. GTP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHPYL85963:GJB9-717-MONOMER.
UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic pyranopterin monophosphate synthaseUniRule annotation (EC:4.1.99.18UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Ordered Locus Names:jhp_0705
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000804 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. molybdopterin synthase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321Cyclic pyranopterin monophosphate synthasePRO_0000152968Add
BLAST

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi85963.jhp0705.

Structurei

3D structure databases

ProteinModelPortaliQ9ZL75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni254 – 2563GTP bindingUniRule annotation

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2896.
KOiK03639.
OMAiIRCAHID.
OrthoDBiEOG64XXNN.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZL75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVDSFNRVI DYIRVSVTKQ CNFRCQYCMP TTPLDFFDDE ELLPLDNVLE
60 70 80 90 100
FLKIAIDEGV KKIRITGGEP LLRKGLDEFI AKLHAYNKEV ALVLSTNGFS
110 120 130 140 150
LKKMAKGLKD AGLSRVNVSL DSLKSDRVLK ISQKDALKNT LEGIEESLKV
160 170 180 190 200
GLKLKLNTVV MKGVNDDEIL ELLEYAKNRS IQIRYIEFME NTHAKSLVKG
210 220 230 240 250
LKEKEILDLI AQKYKIMGME KPKQGSSKIY TLENGYQFGI IAPHSDDFCQ
260 270 280 290 300
SCNRIRLASD GKICPCLYYQ DAIDAKEAII NKDTKMMKRL LKQSIINKPE
310 320
KNMWNDKNGG TPTRAFYYTG G
Length:321
Mass (Da):36,455
Last modified:May 1, 1999 - v1
Checksum:iB7802F6FCC6D2197
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD06280.1.
PIRiA71900.
RefSeqiNP_223423.1. NC_000921.1.
WP_010882557.1. NC_000921.1.

Genome annotation databases

EnsemblBacteriaiAAD06280; AAD06280; jhp_0705.
KEGGihpj:jhp0705.
PATRICi20606133. VBIHelPyl98156_0772.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD06280.1.
PIRiA71900.
RefSeqiNP_223423.1. NC_000921.1.
WP_010882557.1. NC_000921.1.

3D structure databases

ProteinModelPortaliQ9ZL75.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85963.jhp0705.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD06280; AAD06280; jhp_0705.
KEGGihpj:jhp0705.
PATRICi20606133. VBIHelPyl98156_0772.

Phylogenomic databases

eggNOGiCOG2896.
KOiK03639.
OMAiIRCAHID.
OrthoDBiEOG64XXNN.

Enzyme and pathway databases

UniPathwayiUPA00344.
BioCyciHPYL85963:GJB9-717-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J99 / ATCC 700824.

Entry informationi

Entry nameiMOAA_HELPJ
AccessioniPrimary (citable) accession number: Q9ZL75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: April 29, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.