Reviewed, UniProtKB/Swiss-Prot Q9ZL64 (ACON2_HELPJ)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase 2
      Short name=Aconitase 2
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase 2

Gene names

Name:	acnB
Ordered Locus Names:	jhp_0716

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	852 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 852	852	Aconitate hydratase 2		PRO_0000076677
Sites
	Metal binding	708	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	766	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	769	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZL64-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: F9623403AF2556A9	FASTA	852	92,742
	10 20 30 40 50 60 MKDFLEDYKK SVLERKSEGI PPLPLNAKQV EAVVEILMKD PTNAAFAKEL LIHRVSPGVD 70 80 90 100 110 120 EGAKVKAEFL AQLSQKKLEC PHISALEATT LLGTMLGGYN VEPLIVGLES QDKNIAKESA 130 140 150 160 170 180 KALKTTLLVY GSFDKIAAMS KTNALAKEVL ESWANAEWFL NKEPLNECIE ACVFKIDGET 190 200 210 220 230 240 NTDDLSPASD AFTRSDIPLH AKAMLKNRIE NYEQRIEAIK TKGVPVAYVG DVVGTGSSRK 250 260 270 280 290 300 SATNSIMWHF GKDIPFVPNK RSGGIVIGGV IAPIFFATCE DSGALPIVAD VKDLKEGDII 310 320 330 340 350 360 KIYPYKGEIT LNDKVVSTFK LEPETLLDEV RASGRIPLII GRGLTNKARK FLGLGESEAF 370 380 390 400 410 420 KKPSAPKSDA KGYTLAQKIV GHACGVKGIL PGAYCEPKVT TVGSQDTTGA MTRDEVKELA 430 440 450 460 470 480 SLKFDAPFVL QSFCHTAAYP KPSDVSLHAT LPGFITQRGG VALHPGDGVI HTWLNRMGLP 490 500 510 520 530 540 DTLGTGGDSH TRFPLGISFP AGSGLVAFAA VTGTMPLNMP ESVLVRFKGE MNPGITLRDL 550 560 570 580 590 600 VNAIPYYAIK KGLLTVEKKG KINVFNGRIL EIEGLPDIKM EQAFELSDAS AERSAAACVV 610 620 630 640 650 660 RLNKEPMIEY LKSNIKLIDE MIASGYEDKE TLKKRRDAMQ AWVDKPVLLE PDSNAQYAAV 670 680 690 700 710 720 IEIDVAEITE PILACPNDPD DVATLSEVLA DTTGKRPHAI DEVFIGSCMT NIGHFRAFGE 730 740 750 760 770 780 IVKNAPPSQA RLWVVPPSKM DEQELINEGY YAIFGAAGAR TEVPGCSLCM GNQARVRDNA 790 800 810 820 830 840 VVFSTSTRNF DNRMGRGAKV YLGSAELGAA CALLGRIPTK EEYMNLVSEK LESQKDKIYR 850 YMNFNLMENF RL

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001439 Genomic DNA. Translation: AAD06299.1.
PIR	D71896.
RefSeq	NP_223434.1.
3D structure databases
HSSP	HSSP built from PDB template 1L5J based on UniProtKB P36683.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZL64.
Genome annotation databases
GeneID	890183.
GenomeReviews	Gene locus jhp_0716 in contig AE001439_GR.
KEGG	hpj:jhp0716.
NMPDR	fig|85963.1.peg.712.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZL64.
OMA	SATNSIM.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0716-MON.
BRENDA	4.2.1.3. 295085.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR004406. Aconitase_B_bac. IPR015930. Aconitase_B_FeS-bd_bac. IPR015933. Aconitase_B_HEAT-like_bac. IPR015929. Aconitase_B_N_bac. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit. G3DSA:1.25.40.160. Aconitase_B_HEAT-like_bac. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF3. Aconitase_2. 1 hit.
Pfam	PF00330. Aconitase. 2 hits. PF06434. Aconitase_2_N. 1 hit. [Graphical view]
PIRSF	PIRSF036687. AcnB. 1 hit.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00117. acnB. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON2_HELPJ

Accession

Primary (citable) accession number: Q9ZL64

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)