Reviewed, UniProtKB/Swiss-Prot Q9ZL14 (IMDH_HELPJ)

Names and origin

Protein names

Recommended name:
    Inosine-5'-monophosphate dehydrogenase
      Short name=IMP dehydrogenase
      Short name=IMPDH
      Short name=IMPD
    EC=1.1.1.205

Gene names

Name:	guaB
Ordered Locus Names:	jhp_0768

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.
Pathway	Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the IMPDH/GMPR family. Contains 2 CBS domains.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	CBS domain Repeat
Ligand	Metal-binding NAD Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	IMP dehydrogenase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Inosine-5'-monophosphate dehydrogenase		PRO_0000093699
Regions
	Domain	92 – 148	57	CBS 1
	Domain	152 – 209	58	CBS 2
	Nucleotide binding	225 – 246	22	NAD By similarity
Sites
	Active site	300	1	Thioimidate intermediate By similarity
	Metal binding	295	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	297	1	Potassium; via carbonyl oxygen By similarity
	Binding site	333	1	IMP By similarity
	Binding site	380	1	IMP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZL14-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 688262DCD14EFDAB	FASTA	481	51,689
	10 20 30 40 50 60 MRILQRALTF EDVLMVPRKS SVLPKDVSLK SRLTKNIGLN IPFISAAMDT VTEHKTAIAM 70 80 90 100 110 120 ARLGGIGIVH KNMDIQTQVK EITKVKKSES GVINDPIFIH AHRTLADAKV ITDNYKISGV 130 140 150 160 170 180 PVVDDKGLLI GILTNRDVRF ETDLSKKVGD VMTKMPLVTA HVGISLDEAS DLMHKHKIEK 190 200 210 220 230 240 LPIVDKDNVL KGLITIKDIQ KRIEYPEANK DDFGRLRVGA AIGVGQLDRA EMLVKAGVDA 250 260 270 280 290 300 LVLDSAHGHS ANILHTLEEI KKSLVVDVIV GNVVTKEATS DLISAGADAV KVGIGPGSIC 310 320 330 340 350 360 TTRIVAGVGM PQVSAIDNCV EVASKFDIPV IADGGIRYSG DVAKALALGA SSVMIGSLLA 370 380 390 400 410 420 GTEESPGDFM IYQGRQYKSY RGMGSIGAMT KGSSDRYFQE GVASEKLVPE GIEGRVPYRG 430 440 450 460 470 480 KVSDMIFQLV GGVRSSMGYQ GAKNILELYQ NAEFVEITSA GLKESHVHGV DITKEAPNYY G

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001439 Genomic DNA. Translation: AAD06347.1.
PIR	H71890.
RefSeq	NP_223486.1.
3D structure databases
HSSP	HSSP built from PDB template 1EEP based on UniProtKB P49058.
ModBase	Search...
Genome annotation databases
GeneID	890128.
GenomeReviews	Gene locus jhp_0768 in contig AE001439_GR.
KEGG	hpj:jhp0768.
NMPDR	fig|85963.1.peg.764.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZL14.
OMA	Q9ZL14. CVGLITV.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0768-MON.
BRENDA	1.1.1.205. 295085.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000644. Cysta_beta_synth_core. IPR005990. IMP_DH. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. IPR018529. IMP_DH_rel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00571. CBS. 1 hit. PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000130. IMPDH. 1 hit.
SMART	SM00116. CBS. 2 hits. [Graphical view]
TIGRFAMs	TIGR01302. IMP_dehydrog. 1 hit.
PROSITE	PS51371. CBS. 2 hits. PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IMDH_HELPJ

Accession

Primary (citable) accession number: Q9ZL14

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)