Q9ZKZ9 (THIM_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	jhp_0783

Organism

Helicobacter pylori (strain J99) (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Hydroxyethylthiazole kinase HAMAP-Rule MF_00228		PRO_0000156938
Sites
	Binding site	37	1	Substrate; via amide nitrogen By similarity
	Binding site	113	1	ATP By similarity
	Binding site	158	1	ATP By similarity
	Binding site	185	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZKZ9 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2838CA8483C42000	FASTA	259	27,646
	10 20 30 40 50 60 MLKELRQKRP LVHNITNYVV AQFVANGLLA LGVSPLMSDA IAEMPDLAKI SDALAINIGT 70 80 90 100 110 120 LNERTILCAK EAIKRYKALN KPIVLDPVGC SASALRYDTS LELLESKGIS TLRGNAAELG 130 140 150 160 170 180 SLVGISCESK GLDSKHSATP IEIVKRAAQK YSVIAVMTGK TDYVSDGKKV LSITGGSEYL 190 200 210 220 230 240 AAITGAGCLH AAACASFLSL KKDPLDSMVQ LCAFYKQAAF SAQKKALENN GSNGSFLFYF 250 LDALSLPIEL ENSLIKEEL

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD06352.1.
PIR	A71890.
RefSeq	NP_223501.1. NC_000921.1.
3D structure databases
ProteinModelPortal	Q9ZKZ9.
ModBase	Search...
Protein-protein interaction databases
STRING	85963.jhp0783.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD06352; AAD06352; jhp_0783.
GeneID	890114.
KEGG	hpj:jhp0783.
PATRIC	20606299. VBIHelPyl98156_0854.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
KO	K00878.
OMA	SPVMAHA.
ProtClustDB	PRK09355.
Enzyme and pathway databases
BioCyc	HPYL85963:GJB9-796-MONOMER.
UniPathway	UPA00060; UER00139.
Family and domain databases
HAMAP	MF_00228. Thz_kinase.
InterPro	IPR000417. Hyethyz_kinase. [Graphical view]
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_HELPJ

Accession

Primary (citable) accession number: Q9ZKZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program