Q9ZKZ2 (GUAC_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP reductase
EC=1.7.1.7

Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase
Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	jhp_0790

Organism

Helicobacter pylori (strain J99) (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	GMP reductase HAMAP MF_01511		PRO_0000093757
Regions
	Nucleotide binding	203 – 226	24	NADP By similarity
Sites
	Active site	174	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZKZ2 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 86F1FCF53DE69E91	FASTA	325	35,845
	10 20 30 40 50 60 MKVFDYEDVQ LIPNKCIVNS RSECDTTVIL GKHAFKMPIV PANMQTIINE SIAEFLAENG 70 80 90 100 110 120 YFYIMHRFNG SARIPFVKKM KERQLISSIS VGVKKEECLF VEELAKQGLT PDYITIDIAH 130 140 150 160 170 180 GHSNSVIEMI QRIKTRLPET FVIAGNVGTP EAVRELENAG ADATKVGIGP GKVCITKIKT 190 200 210 220 230 240 GFGTGGWQLA ALRWCAKAAR KPIIADGGIR THGDIAKSIR FGATMVMIGS LFAGHEESSG 250 260 270 280 290 300 ETKIENGIAY KEYFGSASEF QKGEKKNIEG KKIWIQHKGS LKNTLIEMHQ DLQSSISYAG 310 320 GRDLEAIRKV DYVIVKNSIF NGDTI

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J99.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD06382.1.
PIR	A71887.
RefSeq	NP_223508.1. NC_000921.1.
3D structure databases
ProteinModelPortal	Q9ZKZ2.
SMR	Q9ZKZ2. Positions 3-320.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZKZ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	890104.
GenomeReviews	Gene locus jhp_0790 in contig AE001439_GR.
KEGG	hpj:jhp0790.
NMPDR	fig|85963.1.peg.786.
PATRIC	20606315. VBIHelPyl98156_0862.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0516.
HOGENOM	HBG298985.
OMA	PDYITID.
ProtClustDB	PRK05458.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP0790-MONOMER.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00364.
PANTHER	PTHR11911:SF30. PTHR11911:SF30. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_HELPJ

Accession

Primary (citable) accession number: Q9ZKZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program