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Q9ZKD8 (DHE4_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-specific glutamate dehydrogenase

Short name=NADP-GDH
EC=1.4.1.4
Gene names
Name:gdhA
Ordered Locus Names:jhp_1001
OrganismHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99) [Complete proteome] [HAMAP]
Taxonomic identifier85963 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia By similarity.

Catalytic activity

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Subunit structure

Homohexamer By similarity.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamate biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionglutamate dehydrogenase (NADP+) activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 448448NADP-specific glutamate dehydrogenase
PRO_0000182772

Sites

Active site1241Proton donor By similarity
Binding site881Substrate By similarity
Binding site1091Substrate By similarity
Binding site1121Substrate By similarity
Binding site1631Substrate; via carbonyl oxygen By similarity
Binding site2071NADP By similarity
Binding site2381NADP By similarity
Binding site3811Substrate By similarity
Site1641Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZKD8 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 1E25BAC991C4FCCB

FASTA44849,491
        10         20         30         40         50         60 
MYVEKILQSL QKKYPYQKEF HQAVYEAITS LKPLLDSDKS YEKHAVLERL IEPEREIFFR 

        70         80         90        100        110        120 
VCWLDDNHQI QVNRGCRVEF NSAIGPYKGG LRFHPSVNES VIKFLGFEQV LKNSLTTLAM 

       130        140        150        160        170        180 
GGAKGGSDFD PKEKSEHEIM RFCQAFMNEL YRHIGATTDV PAGDIGVGER EIGYLFGQYK 

       190        200        210        220        230        240 
KLVNRFEGVL TGKGLTYGGS LCRKEATGYG CVYFAEEMLQ ERNSSLEGKV CSVSGSGNVA 

       250        260        270        280        290        300 
IYTIEKLLQI GAKPVTASDS NGMIYDKDGI DLELLKEIKE ARRGRIKEYA LEKTSAKYTP 

       310        320        330        340        350        360 
TENYPKGGNA IWHVPCFAAF PSATENELSV LDAKTLLSNG CKCVAEGANM PSSNEAIELF 

       370        380        390        400        410        420 
LQAKISYGIG KAANAGGVSV SGLEMAQNAS MHPWSFEVVD AKLHHIMKEI YKNVSQTAKE 

       430        440 
FKDPTNFVLG ANIAGFRKVA SAMIAQGV 

« Hide

References

[1]"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori."
Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. expand/collapse author list , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.
Nature 397:176-180(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J99 / ATCC 700824.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001439 Genomic DNA. Translation: AAD06574.1.
PIRF71862.
RefSeqNP_223718.1. NC_000921.1.

3D structure databases

ProteinModelPortalQ9ZKD8.
SMRQ9ZKD8. Positions 2-448.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9ZKD8. 1 interaction.
STRING85963.jhp1001.

Proteomic databases

PRIDEQ9ZKD8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD06574; AAD06574; jhp_1001.
GeneID889639.
KEGGhpj:jhp1001.
PATRIC20606792. VBIHelPyl98156_1089.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0334.
KOK00262.
OMAVAIYTIE.
OrthoDBEOG65XN4D.
ProtClustDBPRK09414.

Enzyme and pathway databases

BioCycHPYL85963:GJB9-1026-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE4_HELPJ
AccessionPrimary (citable) accession number: Q9ZKD8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: November 13, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families