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Protein

NADP-specific glutamate dehydrogenase

Gene

gdhA

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.By similarity

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881SubstrateBy similarity
Binding sitei109 – 1091SubstrateBy similarity
Binding sitei112 – 1121SubstrateBy similarity
Active sitei124 – 1241Proton donorPROSITE-ProRule annotation
Binding sitei163 – 1631Substrate; via carbonyl oxygenBy similarity
Sitei164 – 1641Important for catalysisBy similarity
Binding sitei207 – 2071NADPBy similarity
Binding sitei238 – 2381NADPBy similarity
Binding sitei381 – 3811SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciHPYL85963:GJB9-1026-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Gene namesi
Name:gdhA
Ordered Locus Names:jhp_1001
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000804 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448NADP-specific glutamate dehydrogenasePRO_0000182772Add
BLAST

Proteomic databases

PRIDEiQ9ZKD8.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

IntActiQ9ZKD8. 1 interaction.
STRINGi85963.jhp1001.

Structurei

3D structure databases

ProteinModelPortaliQ9ZKD8.
SMRiQ9ZKD8. Positions 2-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0334.
KOiK00262.
OMAiVTWMDDK.
OrthoDBiEOG65XN4D.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZKD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYVEKILQSL QKKYPYQKEF HQAVYEAITS LKPLLDSDKS YEKHAVLERL
60 70 80 90 100
IEPEREIFFR VCWLDDNHQI QVNRGCRVEF NSAIGPYKGG LRFHPSVNES
110 120 130 140 150
VIKFLGFEQV LKNSLTTLAM GGAKGGSDFD PKEKSEHEIM RFCQAFMNEL
160 170 180 190 200
YRHIGATTDV PAGDIGVGER EIGYLFGQYK KLVNRFEGVL TGKGLTYGGS
210 220 230 240 250
LCRKEATGYG CVYFAEEMLQ ERNSSLEGKV CSVSGSGNVA IYTIEKLLQI
260 270 280 290 300
GAKPVTASDS NGMIYDKDGI DLELLKEIKE ARRGRIKEYA LEKTSAKYTP
310 320 330 340 350
TENYPKGGNA IWHVPCFAAF PSATENELSV LDAKTLLSNG CKCVAEGANM
360 370 380 390 400
PSSNEAIELF LQAKISYGIG KAANAGGVSV SGLEMAQNAS MHPWSFEVVD
410 420 430 440
AKLHHIMKEI YKNVSQTAKE FKDPTNFVLG ANIAGFRKVA SAMIAQGV
Length:448
Mass (Da):49,491
Last modified:May 1, 1999 - v1
Checksum:i1E25BAC991C4FCCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD06574.1.
PIRiF71862.
RefSeqiNP_223718.1. NC_000921.1.
WP_000289195.1. NC_000921.1.

Genome annotation databases

EnsemblBacteriaiAAD06574; AAD06574; jhp_1001.
KEGGihpj:jhp1001.
PATRICi20606792. VBIHelPyl98156_1089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD06574.1.
PIRiF71862.
RefSeqiNP_223718.1. NC_000921.1.
WP_000289195.1. NC_000921.1.

3D structure databases

ProteinModelPortaliQ9ZKD8.
SMRiQ9ZKD8. Positions 2-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9ZKD8. 1 interaction.
STRINGi85963.jhp1001.

Proteomic databases

PRIDEiQ9ZKD8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD06574; AAD06574; jhp_1001.
KEGGihpj:jhp1001.
PATRICi20606792. VBIHelPyl98156_1089.

Phylogenomic databases

eggNOGiCOG0334.
KOiK00262.
OMAiVTWMDDK.
OrthoDBiEOG65XN4D.

Enzyme and pathway databases

BioCyciHPYL85963:GJB9-1026-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J99 / ATCC 700824.

Entry informationi

Entry nameiDHE4_HELPJ
AccessioniPrimary (citable) accession number: Q9ZKD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: May 27, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.