Reviewed, UniProtKB/Swiss-Prot Q9ZKD8 (DHE4_HELPJ)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4

Gene names

Name:	gdhA
Ordered Locus Names:	jhp_1001

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	NADP-specific glutamate dehydrogenase		PRO_0000182772
Sites
	Active site	124	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZKD8-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 1E25BAC991C4FCCB	FASTA	448	49,491
	10 20 30 40 50 60 MYVEKILQSL QKKYPYQKEF HQAVYEAITS LKPLLDSDKS YEKHAVLERL IEPEREIFFR 70 80 90 100 110 120 VCWLDDNHQI QVNRGCRVEF NSAIGPYKGG LRFHPSVNES VIKFLGFEQV LKNSLTTLAM 130 140 150 160 170 180 GGAKGGSDFD PKEKSEHEIM RFCQAFMNEL YRHIGATTDV PAGDIGVGER EIGYLFGQYK 190 200 210 220 230 240 KLVNRFEGVL TGKGLTYGGS LCRKEATGYG CVYFAEEMLQ ERNSSLEGKV CSVSGSGNVA 250 260 270 280 290 300 IYTIEKLLQI GAKPVTASDS NGMIYDKDGI DLELLKEIKE ARRGRIKEYA LEKTSAKYTP 310 320 330 340 350 360 TENYPKGGNA IWHVPCFAAF PSATENELSV LDAKTLLSNG CKCVAEGANM PSSNEAIELF 370 380 390 400 410 420 LQAKISYGIG KAANAGGVSV SGLEMAQNAS MHPWSFEVVD AKLHHIMKEI YKNVSQTAKE 430 440 FKDPTNFVLG ANIAGFRKVA SAMIAQGV

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001439 Genomic DNA. Translation: AAD06574.1.
PIR	F71862.
RefSeq	NP_223718.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Genome annotation databases
GeneID	889639.
GenomeReviews	Gene locus jhp_1001 in contig AE001439_GR.
KEGG	hpj:jhp1001.
NMPDR	fig|85963.1.peg.996.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZKD8.
OMA	Q9ZKD8. EMAQNAS.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP1001-MON.
BRENDA	1.4.1.4. 295085.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_HELPJ

Accession

Primary (citable) accession number: Q9ZKD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)