Aspartate-semialdehyde dehydrogenase - Helicobacter pylori (strain J99)

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Q9ZK28 (DHAS_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 80. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Aspartate-semialdehyde dehydrogenase
Short name=ASA dehydrogenase
Short name=ASADH
EC=1.2.1.11

Alternative name(s):
Aspartate-beta-semialdehyde dehydrogenase

Gene names

Name:	asd
Ordered Locus Names:	jhp_1114

Organism

Helicobacter pylori (strain J99) (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate By similarity. HAMAP MF_02121
Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP⁺ = L-4-aspartyl phosphate + NADPH. HAMAP MF_02121
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4. HAMAP MF_02121 Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. HAMAP MF_02121 Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5. HAMAP MF_02121
Subunit structure	Homodimer By similarity. HAMAP MF_02121
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis Methionine biosynthesis Threonine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoleucine biosynthetic process Inferred from electronic annotation. Source: InterPro methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	N-acetyl-gamma-glutamyl-phosphate reductase activity Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 346

346

Aspartate-semialdehyde dehydrogenase HAMAP MF_02121

PRO_0000141377

Regions

Nucleotide binding

12 – 15

NADP By similarity

Nucleotide binding

40 – 41

NADP By similarity

Nucleotide binding

161 – 162

NADP By similarity

Sites

Active site

131

Acyl-thioester intermediate By similarity

Active site

253

Proton acceptor By similarity

Binding site

101

Phosphate By similarity

Binding site

158

Substrate By similarity

Binding site

225

Phosphate By similarity

Binding site

246

Substrate By similarity

Binding site

326

NADP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9ZK28 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 76A7A7C3C4EF6287
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FASTA

346

38,131

        10         20         30         40         50         60 
MKTYNVAIVG ASGAVGQELI KGLENSFFPI KKFVPLASAR SAGKKIRAFN KDYEILETTH 

        70         80         90        100        110        120 
EVFEKEEIDI AFFSAGGSVS EEFAISASKT ALVIDNTSFF RLNKDVPLVV PEINAQEIFN 

       130        140        150        160        170        180 
APLNIIANPN CSTIQMTQIL NPLHLHFKIK SVIVSTYQAV SGAGNKGIES LKNELKTALE 

       190        200        210        220        230        240 
HLEKDPAIDL NQVLQAGAFA YPIAFNAIAH IDTFKENGYT KEELKMVHET HKIMGVDFPI 

       250        260        270        280        290        300 
SATCVRVPVL RSHSESLSIA FEKEFDLKEV YEVLKNAPSV VVCDDPSHNL YPTPLKASHT 

       310        320        330        340 
DSVFIGRLRK DLFDKKTLHG FCVADQLRVG AATNALKIAL HYIKNA

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References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori."
Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.

Trust T.J.
Nature 397:176-180(1999) [PubMed: 9923682] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD06695.1.
PIR	C71847.
RefSeq	NP_223831.1. NC_000921.1.
3D structure databases
ProteinModelPortal	Q9ZK28.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZK28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	889756.
GenomeReviews	Gene locus jhp_1114 in contig AE001439_GR.
KEGG	hpj:jhp1114.
NMPDR	fig\|85963.1.peg.1109.
PATRIC	20607044. VBIHelPyl98156_1210.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0136.
HOGENOM	HBG518238.
OMA	FHGKQVE.
PhylomeDB	Q9ZK28.
ProtClustDB	CLSK496405.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP1114-MONOMER.
Family and domain databases
HAMAP	MF_02121. ASADH. [Tree]
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR012080. Asp_semialdehyde_DH. IPR005986. Asp_semialdehyde_DH_beta. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_dimer_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00133.
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
SMART	SM00859. Semialdhyde_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01296. Asd_B. 1 hit.
PROSITE	PS01103. ASD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_HELPJ

Accession

Primary (citable) accession number: Q9ZK28

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents