Reviewed, UniProtKB/Swiss-Prot Q9ZK07 (PUR2_HELPJ)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	jhp_1140

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151454
Regions
	Domain	111 – 312	202	ATP-grasp
	Nucleotide binding	137 – 189	53	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZK07-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: BB4B8D38A4330836	FASTA	424	47,511
	10 20 30 40 50 60 MKDNNNYNVL IVGNKGREYA LAQRLQQDER VNALYFCLGN GGTQDLGENL ECEHYEHIVE 70 80 90 100 110 120 LALKKQIHLA IISEEEPLIL GLTEMLEKAG ILVFGASKEA AKLEASKSYM KAFVKECGIK 130 140 150 160 170 180 SASYFETNDL KEALNYIQNA SFPLVIKALN KNTSIVHHQE EALKILEDAL KQSNEPVIIE 190 200 210 220 230 240 PFLEGFELSV TALIANDDFI LLPFCQNYKR LLEGDNGVNT GGMGAIAPAN FFSNELEEKI 250 260 270 280 290 300 KNHIFKPTLE KLQADNTPFK GVLLAEIVII EEKGVLEPYL LDFSVRFKDI ECQTILPLVE 310 320 330 340 350 360 NPLLDLFLAT AKGELHSLEL VFSKEFVMSV ALVSRNYPTS SSPKQTLYID PVDEKKGHLI 370 380 390 400 410 420 LGEVEQDNGV FESSGGRVIF AIGRGKSLLE ARNHAYEIAQ KVHFEGMFYR KDIGFKVLDL KEYS

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001439 Genomic DNA. Translation: AAD06709.1.
PIR	A71846.
RefSeq	NP_223858.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZK07.
Genome annotation databases
GeneID	889774.
GenomeReviews	Gene locus jhp_1140 in contig AE001439_GR.
KEGG	hpj:jhp1140.
NMPDR	fig|85963.1.peg.1136.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZK07.
OMA	IEYNCRF.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP1140-MON.
BRENDA	6.3.4.13. 295085.
Family and domain databases
HAMAP	MF_00138. Divergent sequence. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_HELPJ

Accession

Primary (citable) accession number: Q9ZK07

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)