Reviewed, UniProtKB/Swiss-Prot Q9ZJY9 (CARA_HELPJ)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	jhp_1158

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112283
Regions
	Domain	188 – 375	188	Glutamine amidotransferase type-1
	Region	1 – 184	184	CPSase HAMAP MF_01209
Sites
	Active site	268	1	Nucleophile By similarity
	Active site	351	1	By similarity
	Active site	353	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZJY9-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 8F002B333B75B7E2	FASTA	375	41,652
	10 20 30 40 50 60 MVSLYLENGL FLQAQSFGAS GTQVGELVFN TSMSGYQEVI SDPSYKGQFV VFSMPEIGVV 70 80 90 100 110 120 GANPKDDESF FSCAGVLARH YNEFFSNSRA DSSLSTYLKK RGVLGISGVD TRSLIKTLRH 130 140 150 160 170 180 HGCLMMIAST IEHDRNKLEE VLKNAPKISH SPLVSSVSTP KIITHQRATF DFNTLDYKPF 190 200 210 220 230 240 DEKIPHKIIA VLDFGAKGNI LNELQNVGLK ALIYPHHTKA NELIKAYEKK EISGIFLSNG 250 260 270 280 290 300 PGDPLSLQQE IGEIKRLINA KIPMFGICLG HQLLSIAQGY PTYKLKFGHH GSNHPVKNLE 310 320 330 340 350 360 TNAVEITAQN HNYCVPEEIE EIATITHRNL FDNTIEGVRY KNAPIISVQH HPESSPGPKE 370 SHYIFKEFVG LLEGF

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD06735.1.
PIR	B71842.
RefSeq	NP_223876.1.
3D structure databases
SMR	Q9ZJY9. Positions 3-374.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZJY9.
Genome annotation databases
GeneID	889793.
GenomeReviews	Gene locus jhp_1158 in contig AE001439_GR.
KEGG	hpj:jhp1158.
NMPDR	fig|85963.1.peg.1154.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0505.
HOGENOM	HBG286341.
OMA	EADIPFF.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP1158-MONOMER.
BRENDA	6.3.5.5. 295085.
Family and domain databases
HAMAP	MF_01209_B. CPSase_S_chain_B. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_HELPJ

Accession

Primary (citable) accession number: Q9ZJY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)