Q9ZJY5 (SYA_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	jhp_1162

Organism

Helicobacter pylori (strain J99) (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	847 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP-Rule MF_00036
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP-Rule MF_00036
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00036
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00036.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP-Rule MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 847	847	Alanine--tRNA ligase HAMAP-Rule MF_00036		PRO_0000075126
Sites
	Metal binding	554	1	Zinc Potential
	Metal binding	558	1	Zinc Potential
	Metal binding	656	1	Zinc Potential
	Metal binding	660	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9ZJY5 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 714B10CA1F92D8F1	FASTA	847	94,978
	10 20 30 40 50 60 MDIRNEFLQF FQNKGHEIYP SMPLVPNDAT LLFTNAGMVQ FKDIFTGIVP RPSIPRAASS 70 80 90 100 110 120 QLCMRAGGKH NDLENVGYTA RHHTLFEMLG NFSFGDYFKE EAILFAWEFV TKNLGFKPKD 130 140 150 160 170 180 LYISVHEKDD EAVKLWEKFV PVDRIKKMGD KDNFWQMGDS GPCGPCSEIY IDQGEKHFKG 190 200 210 220 230 240 SEDYFGGEGD RFLEIWNLVF MQYERSNDGV LSPLPKPSID TGMGLERVQA LLEHKLNNFD 250 260 270 280 290 300 SSLFAPLMEE ISELTGLDYA SEFQPSFRVV ADHARAVAFL LAQEVHFNKE GRGYVLRRIL 310 320 330 340 350 360 RRALRHGYLM GLKEAFLYKV VGVVCEQFSN VHAYLKESKE MVMKECFEEE ERFLETLESG 370 380 390 400 410 420 MELFNLSLTH LNENKIFDGK IAFKLYDTFG FPLDLTNDML RSHGACVDMQ GFENCMQEQV 430 440 450 460 470 480 KRSKASWKGK QNNADFSAIL NAYAPNEFVG YETTECLANA LGFFDSDFKE ITEANPNQEV 490 500 510 520 530 540 WVLLEKTPFY AEGGGAIGDR GTLFKDNEEA ALVLDTKNFF GLNFSLLEIK KALKKGDQVI 550 560 570 580 590 600 AQVSDERLEI AKHHSATHLL QSALREVLGS HVSQAGSLVE SKRLRFDFSH AKALNDEELE 610 620 630 640 650 660 KVEDLVNAQI FKHLTSQVEH MPLNQAKDKG ALALFSEKYA ENVRVVSFKE ASIELCGGIH 670 680 690 700 710 720 VENTGLIGGF RIVKESGVSS GVRRIEAVCG KAFYQLTKEE NKELKNAKTL LKNNDVIAGI 730 740 750 760 770 780 NKLKESVKNS QKAPVSMDLP VEKIHGVNLV VGVVEQGDIK EMIDRLKSKH EKLLAIVFKK 790 800 810 820 830 840 ENERITLACG VKNVPIKANA WANEVAQILG GKGGGRDDFA SAGGKDIENL QAALNLAKNT ALKALEG

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD06738.1.
PIR	F71842.
RefSeq	NP_223880.1. NC_000921.1.
3D structure databases
ProteinModelPortal	Q9ZJY5.
ModBase	Search...
Protein-protein interaction databases
STRING	85963.jhp1162.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD06738; AAD06738; jhp_1162.
GeneID	889797.
KEGG	hpj:jhp1162.
PATRIC	20607162. VBIHelPyl98156_1262.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
KO	K01872.
OMA	ISQPSIR.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	HPYL85963:GJB9-1198-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B.
InterPro	IPR002318. Ala-tRNA-lgiase_IIc. IPR018162. Ala-tRNA-ligase_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_ligase_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_HELPJ

Accession

Primary (citable) accession number: Q9ZJY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program