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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135L-tryptophanUniRule annotation1
Binding sitei186ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 13ATPUniRule annotation3
Nucleotide bindingi19 – 20ATPUniRule annotation2
Nucleotide bindingi147 – 149ATPUniRule annotation3
Nucleotide bindingi195 – 199ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPYL85963:G1G1A-1266-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:jhp_1174
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000804 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366381 – 326Tryptophan--tRNA ligaseAdd BLAST326

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi85963.jhp1174

Structurei

3D structure databases

ProteinModelPortaliQ9ZJX4
SMRiQ9ZJX4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 20"HIGH" regionUniRule annotation9
Motifi195 – 199"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
KOiK01867
OMAiQRMTQFK
OrthoDBiPOG091H00I9

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZJX4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKKRVFSGI QPTGQIHLGN YLGAIKHWVE LQDEYENLFC IVNSHAITLP
60 70 80 90 100
IEPIFLKSQT YELVKLLLAC GISPKQSGLF IQSEVDEHPA LAWLLDCQVS
110 120 130 140 150
MGEMQRMTQF KDKSLKNPKS VNVGLFNYPI LMASDILLYQ SDLVPVGEDQ
160 170 180 190 200
KQHLELTRNV AEKFNRDFGN CFKVPEPLIA KVGARVMGLD DPKVKMSKSH
210 220 230 240 250
KGANHAIFLL DEPDVIVKKI KKAATDSMGV IAFDETREGV FNLLNIYMLL
260 270 280 290 300
SDESPEKIEE RFRNKGYGDF KKELAEVVIQ SLKPIQERYQ EISDDEVKAV
310 320
LNCGAEKARP LARATYQKAK ELMGLI
Length:326
Mass (Da):36,856
Last modified:May 1, 1999 - v1
Checksum:iB99F0EFC9E9FA491
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA Translation: AAD06748.1
PIRiE71841
RefSeqiWP_010882615.1, NZ_CP011330.1

Genome annotation databases

EnsemblBacteriaiAAD06748; AAD06748; jhp_1174
KEGGihpj:jhp_1174
PATRICifig|85963.30.peg.1398

Similar proteinsi

Entry informationi

Entry nameiSYW_HELPJ
AccessioniPrimary (citable) accession number: Q9ZJX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health