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Q9ZJX4 (SYW_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:jhp_1174
OrganismHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99) [Complete proteome] [HAMAP]
Taxonomic identifier85963 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140_B

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140_B

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140_B.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Tryptophan--tRNA ligase HAMAP-Rule MF_00140_B
PRO_0000136638

Regions

Motif12 – 209"HIGH" region HAMAP-Rule MF_00140_B
Motif195 – 1995"KMSKS" region HAMAP-Rule MF_00140_B

Sites

Binding site1981ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZJX4 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: B99F0EFC9E9FA491

FASTA32636,856
        10         20         30         40         50         60 
MQKKRVFSGI QPTGQIHLGN YLGAIKHWVE LQDEYENLFC IVNSHAITLP IEPIFLKSQT 

        70         80         90        100        110        120 
YELVKLLLAC GISPKQSGLF IQSEVDEHPA LAWLLDCQVS MGEMQRMTQF KDKSLKNPKS 

       130        140        150        160        170        180 
VNVGLFNYPI LMASDILLYQ SDLVPVGEDQ KQHLELTRNV AEKFNRDFGN CFKVPEPLIA 

       190        200        210        220        230        240 
KVGARVMGLD DPKVKMSKSH KGANHAIFLL DEPDVIVKKI KKAATDSMGV IAFDETREGV 

       250        260        270        280        290        300 
FNLLNIYMLL SDESPEKIEE RFRNKGYGDF KKELAEVVIQ SLKPIQERYQ EISDDEVKAV 

       310        320 
LNCGAEKARP LARATYQKAK ELMGLI 

« Hide

References

[1]"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori."
Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. expand/collapse author list , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.
Nature 397:176-180(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J99 / ATCC 700824.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001439 Genomic DNA. Translation: AAD06748.1.
PIRE71841.
RefSeqNP_223892.1. NC_000921.1.

3D structure databases

ProteinModelPortalQ9ZJX4.
SMRQ9ZJX4. Positions 4-326.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85963.jhp1174.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD06748; AAD06748; jhp_1174.
GeneID889808.
KEGGhpj:jhp1174.
PATRIC20607188. VBIHelPyl98156_1275.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
KOK01867.
OMAIDPKKHI.
OrthoDBEOG686NJQ.

Enzyme and pathway databases

BioCycHPYL85963:GJB9-1210-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_HELPJ
AccessionPrimary (citable) accession number: Q9ZJX4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries