Q9ZJX0 (PYRE_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	jhp_1178

Organism

Helicobacter pylori (strain J99) (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110702
Regions
	Region	113 – 121	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	117	1	Orotate By similarity
	Binding site	145	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZJX0 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 8C774BD9616D97DC	FASTA	201	21,933
	10 20 30 40 50 60 MDIKACYQNA QALLEGHFLL SSGFHSNYYL QSAKVLEDPK LAEQLALELA KQIQEAHLNI 70 80 90 100 110 120 ECVCSPAIGG ILAGYELARA LGVRFIFTER VDNTMTLRRG FEVKKNEKIL VCEDIITTGK 130 140 150 160 170 180 SAMECAKVLE EKGAQIVAFG ALANRGICKR AHSHLKAQEG ACLPSHLPLF ALEDFVFDMH 190 200 KPSSCPLCAT SVAIKPGSRG N

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD06764.1.
PIR	G71837.
RefSeq	NP_223896.1. NC_000921.1.
3D structure databases
ProteinModelPortal	Q9ZJX0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZJX0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	889811.
GenomeReviews	Gene locus jhp_1178 in contig AE001439_GR.
KEGG	hpj:jhp1178.
NMPDR	fig|85963.1.peg.1174.
PATRIC	20607196. VBIHelPyl98156_1279.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0461.
HOGENOM	HBG404341.
OMA	FYLQSAK.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP1178-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR023031. Orotate_PribosylTferase. IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. PyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_HELPJ

Accession

Primary (citable) accession number: Q9ZJX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program