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Protein

Methionine aminopeptidase

Gene

map

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78SubstrateUniRule annotation1
Metal bindingi95Divalent metal cation 1UniRule annotation1
Metal bindingi106Divalent metal cation 1UniRule annotation1
Metal bindingi106Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi169Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei176SubstrateUniRule annotation1
Metal bindingi206Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi237Divalent metal cation 1UniRule annotation1
Metal bindingi237Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Ordered Locus Names:jhp_1219
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000804 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489421 – 253Methionine aminopeptidaseAdd BLAST253

Proteomic databases

PRIDEiQ9ZJT0

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi85963.jhp1219

Structurei

3D structure databases

ProteinModelPortaliQ9ZJT0
SMRiQ9ZJT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA1 Bacteria
COG0024 LUCA
KOiK01265
OMAiSYFHGPP
OrthoDBiPOG091H01DX

Family and domain databases

CDDicd01086 MetAP1, 1 hit
HAMAPiMF_01974 MetAP_1, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002467 Pept_M24A_MAP1
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00500 met_pdase_I, 1 hit
PROSITEiView protein in PROSITE
PS00680 MAP_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZJT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAISIKSPKE IKALRKAGEL TAQALALLER EVRPGVSLLE LDKMAEDFIK
60 70 80 90 100
SSHARPAFKG LYGFPNSVCM SLNEVVIHGI PTDYVLQEGD IIGLDLGVEV
110 120 130 140 150
DGYYGDSALT LPIGAISPQD EKLLACSKES LMHAISSIRV GMHFKELSQI
160 170 180 190 200
LEGAITERGF VPLKGFCGHG IGKKPHEEPE IPNYLEKGVK ANSGPKIKEG
210 220 230 240 250
MVFCLEPMVC QKQGEPKILA DKWSVVSVDG LNTSHHEHTI AIVGNKAVIL

TER
Length:253
Mass (Da):27,464
Last modified:May 1, 1999 - v1
Checksum:iD588885F32C34150
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA Translation: AAD06803.1
PIRiC71833
RefSeqiWP_001018132.1, NZ_CP011330.1

Genome annotation databases

EnsemblBacteriaiAAD06803; AAD06803; jhp_1219
KEGGihpj:jhp_1219
PATRICifig|85963.30.peg.1352

Similar proteinsi

Entry informationi

Entry nameiMAP1_HELPJ
AccessioniPrimary (citable) accession number: Q9ZJT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health