Reviewed, UniProtKB/Swiss-Prot Q9ZJL3 (LON_HELPJ)

Names and origin

Protein names

Recommended name:
    ATP-dependent protease La
    EC=3.4.21.53

Gene names

Name:	lon
Ordered Locus Names:	jhp_1293

Organism

Helicobacter pylori J99 (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	831 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins in presence of ATP. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 831	831	ATP-dependent protease La		PRO_0000076138
Regions
	Domain	8 – 200	193	Lon
	Nucleotide binding	367 – 374	8	ATP Potential
	Compositional bias	708 – 712	5	Poly-Lys
Sites
	Active site	737	1	By similarity
	Active site	780	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZJL3-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 1B9249B3C49B1D5E	FASTA	831	93,946
	10 20 30 40 50 60 MTEDFPKILP LLVEEDTFLY PFMIAPIFLQ NNASIKAVAY AKNNKSLVFI ACQKDKLNDN 70 80 90 100 110 120 EAPYYDVGVI GSVMREANMP NGRVKLLFNG IAKGRILEPA KENEQGFLEA QISPIEYLEY 130 140 150 160 170 180 DKENIQAIVE VLKEKVITLA NVSSLFPPDL IKALEDNDDP NRIADLIAAA LHLKKDQAYF 190 200 210 220 230 240 LFANNNTEQR LLDLIDIVIE ETKTQKLQKE IKSKVHQKME QTNKEYFLKE QLKQIQKELG 250 260 270 280 290 300 TDKQRDEDLN QYYQKLESIK PFLKEEAFKE IKKQIDRLSR THADSSDSAT LQNYVETMLD 310 320 330 340 350 360 VPFGQYEKKA LDIKHVKEQL DNDHYSLKRP KERIVEYFAT MQLLEMRHKK KPEKKDKTKG 370 380 390 400 410 420 TILCFYGPPG VGKTSLANSI AKAIERPLVR IALGGLEDVN ELRGHRRTYI GSMPGRIVQG 430 440 450 460 470 480 LIEAKKMNPV MVLDEIDKVD RSVRGDPASA LLEILDPEQN IAFRDHYANF SIDLSQVIFI 490 500 510 520 530 540 ATANNIDRIP APLRDRMEFI SVSSYTPSEK EEIAKNYLIP QELEKHALKP SEVDISHECL 550 560 570 580 590 600 KLIIEKYTRE AGVRDLRRQI ATIMRKAALK YLEDNPHKKG RTKKSEDKDK KGGNEENEKR 610 620 630 640 650 660 GESKDFCVSI TPDNLKEYLE RMVFEIDPID EENKIGIVNG LAWTPVGGDV LKIEAVKIRG 670 680 690 700 710 720 KGELKLTGSL GDVMKESAII AFSVVKVLLD NETLKVPKIP SETDAENKKK KKVLKVYNAY 730 740 750 760 770 780 DLHLHVPEGA TPKDGPSAGI AMASVMASIL CDRAIRSEVA MTGELTLSGE VLPIGGLKEK 790 800 810 820 830 LIAAFKAGIK TALIPVKNYE RDLDEIPTEV RENLNIVAVK NIAEVLEKTL L

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori." Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Nature 397:176-180(1999) [PubMed: 9923682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001439 Genomic DNA. Translation: AAD06875.1.
PIR	A71825.
RefSeq	NP_224011.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	890375.
GenomeReviews	Gene locus jhp_1293 in contig AE001439_GR.
KEGG	hpj:jhp1293.
NMPDR	fig|85963.1.peg.1289.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZJL3.
OMA	Q9ZJL3. IVNGLAW.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP1293-MON.
BRENDA	3.4.21.53. 295085.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_HELPJ

Accession

Primary (citable) accession number: Q9ZJL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)