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Q9ZJK8 (BIOB_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:jhp_1298
OrganismHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99) [Complete proteome] [HAMAP]
Taxonomic identifier85963 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282Biotin synthase HAMAP-Rule MF_01694
PRO_0000185556

Sites

Metal binding171Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding211Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding241Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding961Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1541Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2211Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZJK8 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 3A07177B65AFAF1B

FASTA28231,490
        10         20         30         40         50         60 
MQEIFLCSIS NVRSGDCKED CAYCTQSSHH QGAIKRYKFK DEKVVLQEAR ALRELGALGF 

        70         80         90        100        110        120 
CLVTSGRELD DEKCEYIAKL AKAINKEELG LHLIACCGRA DLEQLEFLRD AGIHSYNHNL 

       130        140        150        160        170        180 
ETSQNFFPKI CTTHTWEERF ITCENALRAG LGLCSGGIFG LNESWEDRIE MLRALASLSP 

       190        200        210        220        230        240 
HTTPINFFIK NPVLPIDAET LSADEALECV LLAKEFLPNA RLMVAGGREV VFKDNDKQEA 

       250        260        270        280 
KLFEYGINAV VLGDYLTTKG KAPKKDIEKL LSYGLTMATS CH 

« Hide

References

[1]"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori."
Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. expand/collapse author list , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.
Nature 397:176-180(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J99 / ATCC 700824.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001439 Genomic DNA. Translation: AAD06876.1.
PIRH71823.
RefSeqNP_224016.1. NC_000921.1.

3D structure databases

ProteinModelPortalQ9ZJK8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85963.jhp1298.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD06876; AAD06876; jhp_1298.
GeneID889874.
KEGGhpj:jhp1298.
PATRIC20607452. VBIHelPyl98156_1407.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMATCENTLR.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycHPYL85963:GJB9-1334-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_HELPJ
AccessionPrimary (citable) accession number: Q9ZJK8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways