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Q9ZJJ1

- SYI_HELPJ

UniProt

Q9ZJJ1 - SYI_HELPJ

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
Binding sitei613 – 6131ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi913 – 9131ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHPYL85963:GJB9-1355-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:jhp_1317
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000804: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 920920Isoleucine--tRNA ligasePRO_0000098399Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi85963.jhp1317.

Structurei

3D structure databases

ProteinModelPortaliQ9ZJJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZJJ1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEYKDTLNL NETTFSMKGN LSVNEPKTYA KWQEQQAFKR MQNRKDNHGD
60 70 80 90 100
FTLHDGPPYA NGHLHLGHAL NKILKDIVIK REYFKGKKIY YTPGWDCHGL
110 120 130 140 150
PIEQQILEQL EKEKTSLENP TLFREKCRDH AKKFLEIQKN EFLQLGVLGD
160 170 180 190 200
FEDPYKTMDF KFEASIYRAL VGVAKKGLLK ERHKPIYWSY ACESALAEAE
210 220 230 240 250
VEYKMKKSPS IFVAFDLKKE GLEKLKVKKA SLVIWTTTPW TLYANVAIAL
260 270 280 290 300
KKDAVYALTQ KGYLVAKALH EKLAALGVVD NAITHEFNSN DLEYLVATNP
310 320 330 340 350
LNQRDSLVVL GDHVSLEDGT GAVHTAPGHG EEDYYLGLKY HLEVLMSVDE
360 370 380 390 400
KGCYDEGIIH NQLLDKSYLG EHIFKAQKRI IEQLGDSLLL EREIEHSYPH
410 420 430 440 450
CWRTHKPVIY RATTQWFILM DEPFIQNDGS QKTLREVALD AIEKVEFVPS
460 470 480 490 500
SGKNRLKTMI ENRPDWCLSR QRKWGVPLAF FIDKRTNKPC FESEVLEHVA
510 520 530 540 550
NLFEEKGCDV WWEYNIKDLL PPSYQDNAKH YEKVMHILDV WFDSGSTFKA
560 570 580 590 600
VLEDYHGEKG QSPSDVVLEG SDQHRGWFQS SLLIGCVLNN QAPFKKVITH
610 620 630 640 650
GFIVDEKGEK MSKSKGNVVS LDNLLKKHGS DVVRLWVAFN DYQNDLRVSQ
660 670 680 690 700
TFFIQTEQHY KKFRNTLKFL LANFSDMDLK NLERPHDFSP LDHFILEALE
710 720 730 740 750
TISAGVNSAF EEHDLVKGLN ILMAFVTNEL SGIYLDACKD SLYCDSKNNE
760 770 780 790 800
KRQAIQMVLL AVASQLCYFL APILTHTIEE VLEHSQVLRA FLQAKDVFDL
810 820 830 840 850
KGISVLEKLH LKEFKKPENF EAVLALRSAF NEELDRLKKE GVVKNSLECA
860 870 880 890 900
IEVKEKALCE NLVEELLMVS FVGVAKEKLS ETPAFTLFKA PFYKCPRCWR
910 920
FKSELENTPC KRCEEVLKER
Length:920
Mass (Da):105,969
Last modified:May 1, 1999 - v1
Checksum:i89B5E507562B470B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001439 Genomic DNA. Translation: AAD06890.1.
PIRiF71823.
RefSeqiNP_224035.1. NC_000921.1.
WP_000666227.1. NC_000921.1.

Genome annotation databases

EnsemblBacteriaiAAD06890; AAD06890; jhp_1317.
GeneIDi889886.
KEGGihpj:jhp1317.
PATRICi20607498. VBIHelPyl98156_1429.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001439 Genomic DNA. Translation: AAD06890.1 .
PIRi F71823.
RefSeqi NP_224035.1. NC_000921.1.
WP_000666227.1. NC_000921.1.

3D structure databases

ProteinModelPortali Q9ZJJ1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 85963.jhp1317.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD06890 ; AAD06890 ; jhp_1317 .
GeneIDi 889886.
KEGGi hpj:jhp1317.
PATRICi 20607498. VBIHelPyl98156_1429.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HPYL85963:GJB9-1355-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J99 / ATCC 700824.

Entry informationi

Entry nameiSYI_HELPJ
AccessioniPrimary (citable) accession number: Q9ZJJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3