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Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway:ipeptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei144 – 1441UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei150 – 1501UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei152 – 1521UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei340 – 3401Meso-diaminopimelateUniRule annotation
Binding sitei415 – 4151Meso-diaminopimelate; via carbonyl oxygenUniRule annotation
Binding sitei419 – 4191Meso-diaminopimelateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 807ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPYL85963:GJB9-1426-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
Gene namesi
Name:murEUniRule annotation
Ordered Locus Names:jhp_1387
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000804 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligasePRO_0000101903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi85963.jhp1387.

Structurei

3D structure databases

ProteinModelPortaliQ9ZJC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni117 – 1182UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation
Regioni364 – 3674Meso-diaminopimelate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi364 – 3674Meso-diaminopimelate recognition motif

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0769.
KOiK01928.
OMAiCEYFIME.
OrthoDBiEOG6PKFCR.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE.
InterProiIPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZJC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKKTLTYQ HHAYSFLSDN TNEVLENPKE ILFVKTPLNE KYAPLIAEKN
60 70 80 90 100
LAILDFNELK NYFDFKIKIV GITGTNGKTT TASLMYSLLL DLNKKTALLG
110 120 130 140 150
TRGFFIDDKH IKEKGLTTPT LLELYSDLEE AIRLKCEYFI MEVSSHAIVQ
160 170 180 190 200
KRIAGLDFAL KILTNITSDH LDFHQNIENY RDAKNSFFKD EGLKVINRDE
210 220 230 240 250
TNALFNPINA RTYALDKKAH LNVQAFSLNP SISASLCYQH DLRDPNLKET
260 270 280 290 300
ALIHSPLLGR YNLYNILAGV LGVKLLTQLP LETIAPLLEN FYGVKGRLEI
310 320 330 340 350
VHSKPLVVVD FAHTTDGMQQ VFESFKNQKI TALFGAGGDR DKTKRPKMGA
360 370 380 390 400
IASCYAHQII LTSDNPRSEN EEDIIKDILK GINNSSKVIV EKDRKKAILN
410 420 430 440
ALENLKDDEV LLILGKGDEN IQIFKDKTIF FSDQEVVKDY YLNLKQG
Length:447
Mass (Da):50,660
Last modified:May 1, 1999 - v1
Checksum:i639AB9D18F3740BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD06968.1.
PIRiD71812.
RefSeqiWP_000768761.1. NC_000921.1.

Genome annotation databases

EnsemblBacteriaiAAD06968; AAD06968; jhp_1387.
KEGGihpj:jhp1387.
PATRICi20607654. VBIHelPyl98156_1506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD06968.1.
PIRiD71812.
RefSeqiWP_000768761.1. NC_000921.1.

3D structure databases

ProteinModelPortaliQ9ZJC6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85963.jhp1387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD06968; AAD06968; jhp_1387.
KEGGihpj:jhp1387.
PATRICi20607654. VBIHelPyl98156_1506.

Phylogenomic databases

eggNOGiCOG0769.
KOiK01928.
OMAiCEYFIME.
OrthoDBiEOG6PKFCR.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciHPYL85963:GJB9-1426-MONOMER.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE.
InterProiIPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J99 / ATCC 700824.

Entry informationi

Entry nameiMURE_HELPJ
AccessioniPrimary (citable) accession number: Q9ZJC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.