UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Contribute

Send feedback

Read comments (?) or add your own

Q9ZJC6 (MURE_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 86. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
EC=6.3.2.13

Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	jhp_1387

Organism

Helicobacter pylori (strain J99) (Campylobacter pylori J99) [Complete proteome] [HAMAP]

Taxonomic identifier

85963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity HAMAP MF_00208.
Post-translational modification	Carbamoylation is probably crucial for Mg²⁺ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208
Sequence similarities	Belongs to the MurCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC tetrahydrofolylpolyglutamate synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 447

447

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208

PRO_0000101903

Regions

Nucleotide binding

74 – 80

ATP Potential

Region

117 – 118

UDP-MurNAc-L-Ala-D-Glu binding By similarity

Region

364 – 367

Meso-diaminopimelate binding By similarity

Motif

364 – 367

Meso-diaminopimelate recognition motif HAMAP MF_00208

Sites

Binding site

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

144

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

150

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

152

UDP-MurNAc-L-Ala-D-Glu By similarity

Binding site

340

Meso-diaminopimelate By similarity

Binding site

415

Meso-diaminopimelate; via carbonyl oxygen By similarity

Binding site

419

Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue

184

N6-carboxylysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9ZJC6 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 639AB9D18F3740BD
Show »

FASTA

447

50,660

        10         20         30         40         50         60 
MKLKKTLTYQ HHAYSFLSDN TNEVLENPKE ILFVKTPLNE KYAPLIAEKN LAILDFNELK 

        70         80         90        100        110        120 
NYFDFKIKIV GITGTNGKTT TASLMYSLLL DLNKKTALLG TRGFFIDDKH IKEKGLTTPT 

       130        140        150        160        170        180 
LLELYSDLEE AIRLKCEYFI MEVSSHAIVQ KRIAGLDFAL KILTNITSDH LDFHQNIENY 

       190        200        210        220        230        240 
RDAKNSFFKD EGLKVINRDE TNALFNPINA RTYALDKKAH LNVQAFSLNP SISASLCYQH 

       250        260        270        280        290        300 
DLRDPNLKET ALIHSPLLGR YNLYNILAGV LGVKLLTQLP LETIAPLLEN FYGVKGRLEI 

       310        320        330        340        350        360 
VHSKPLVVVD FAHTTDGMQQ VFESFKNQKI TALFGAGGDR DKTKRPKMGA IASCYAHQII 

       370        380        390        400        410        420 
LTSDNPRSEN EEDIIKDILK GINNSSKVIV EKDRKKAILN ALENLKDDEV LLILGKGDEN 

       430        440 
IQIFKDKTIF FSDQEVVKDY YLNLKQG

« Hide

References

[1]

"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori."
Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.

Trust T.J.
Nature 397:176-180(1999) [PubMed: 9923682] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J99.

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001439 Genomic DNA. Translation: AAD06968.1.
PIR	D71812.
RefSeq	NP_224105.1. NC_000921.1.
3D structure databases
ProteinModelPortal	Q9ZJC6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ZJC6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	889938.
GenomeReviews	Gene locus jhp_1387 in contig AE001439_GR.
KEGG	hpj:jhp1387.
NMPDR	fig\|85963.1.peg.1383.
PATRIC	20607654. VBIHelPyl98156_1506.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0769.
HOGENOM	HBG602753.
OMA	IGTIANY.
PhylomeDB	Q9ZJC6.
ProtClustDB	PRK00139.
Enzyme and pathway databases
BioCyc	HPYL85963:JHP1387-MONOMER.
Family and domain databases
HAMAP	MF_00208. MurE. [Tree]
InterPro	IPR018109. Folylpolyglutamate_synth_CS. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KO	K01928.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01085. MurE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_HELPJ

Accession

Primary (citable) accession number: Q9ZJC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents