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Q9ZJC6

- MURE_HELPJ

UniProt

Q9ZJC6 - MURE_HELPJ

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Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei144 – 1441UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei150 – 1501UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei152 – 1521UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei340 – 3401Meso-diaminopimelateUniRule annotation
Binding sitei415 – 4151Meso-diaminopimelate; via carbonyl oxygenUniRule annotation
Binding sitei419 – 4191Meso-diaminopimelateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 807ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tetrahydrofolylpolyglutamate synthase activity Source: InterPro
  3. UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. cell wall organization Source: UniProtKB-KW
  4. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  5. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPYL85963:GJB9-1426-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
Gene namesi
Name:murEUniRule annotation
Ordered Locus Names:jhp_1387
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000804: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligasePRO_0000101903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi85963.jhp1387.

Structurei

3D structure databases

ProteinModelPortaliQ9ZJC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni117 – 1182UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation
Regioni364 – 3674Meso-diaminopimelate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi364 – 3674Meso-diaminopimelate recognition motif

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0769.
KOiK01928.
OMAiAVMTNLS.
OrthoDBiEOG6PKFCR.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE.
InterProiIPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZJC6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLKKTLTYQ HHAYSFLSDN TNEVLENPKE ILFVKTPLNE KYAPLIAEKN
60 70 80 90 100
LAILDFNELK NYFDFKIKIV GITGTNGKTT TASLMYSLLL DLNKKTALLG
110 120 130 140 150
TRGFFIDDKH IKEKGLTTPT LLELYSDLEE AIRLKCEYFI MEVSSHAIVQ
160 170 180 190 200
KRIAGLDFAL KILTNITSDH LDFHQNIENY RDAKNSFFKD EGLKVINRDE
210 220 230 240 250
TNALFNPINA RTYALDKKAH LNVQAFSLNP SISASLCYQH DLRDPNLKET
260 270 280 290 300
ALIHSPLLGR YNLYNILAGV LGVKLLTQLP LETIAPLLEN FYGVKGRLEI
310 320 330 340 350
VHSKPLVVVD FAHTTDGMQQ VFESFKNQKI TALFGAGGDR DKTKRPKMGA
360 370 380 390 400
IASCYAHQII LTSDNPRSEN EEDIIKDILK GINNSSKVIV EKDRKKAILN
410 420 430 440
ALENLKDDEV LLILGKGDEN IQIFKDKTIF FSDQEVVKDY YLNLKQG
Length:447
Mass (Da):50,660
Last modified:May 1, 1999 - v1
Checksum:i639AB9D18F3740BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD06968.1.
PIRiD71812.
RefSeqiNP_224105.1. NC_000921.1.
WP_000768761.1. NC_000921.1.

Genome annotation databases

EnsemblBacteriaiAAD06968; AAD06968; jhp_1387.
GeneIDi889938.
KEGGihpj:jhp1387.
PATRICi20607654. VBIHelPyl98156_1506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD06968.1 .
PIRi D71812.
RefSeqi NP_224105.1. NC_000921.1.
WP_000768761.1. NC_000921.1.

3D structure databases

ProteinModelPortali Q9ZJC6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 85963.jhp1387.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD06968 ; AAD06968 ; jhp_1387 .
GeneIDi 889938.
KEGGi hpj:jhp1387.
PATRICi 20607654. VBIHelPyl98156_1506.

Phylogenomic databases

eggNOGi COG0769.
KOi K01928.
OMAi AVMTNLS.
OrthoDBi EOG6PKFCR.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci HPYL85963:GJB9-1426-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPi MF_00208. MurE.
InterProi IPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view ]
Pfami PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view ]
SUPFAMi SSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsi TIGR01085. murE. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J99 / ATCC 700824.

Entry informationi

Entry nameiMURE_HELPJ
AccessioniPrimary (citable) accession number: Q9ZJC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 26, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3