Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9ZJ81 (NADK_HELPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:jhp_1433
OrganismHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99) [Complete proteome] [HAMAP]
Taxonomic identifier85963 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Sequence caution

The sequence AAD07009.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 284284NAD kinase HAMAP-Rule MF_00361
PRO_0000120624

Regions

Nucleotide binding70 – 712NAD By similarity
Nucleotide binding139 – 1402NAD By similarity
Nucleotide binding180 – 1856NAD By similarity

Sites

Active site701Proton acceptor By similarity
Binding site1671NAD By similarity
Binding site1691NAD By similarity
Binding site1771NAD; via carbonyl oxygen By similarity
Binding site2361NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZJ81 [UniParc].

Last modified May 4, 2001. Version 2.
Checksum: E7E4056AC802BA7C

FASTA28431,447
        10         20         30         40         50         60 
MKDLNKTIGV FVRPTHHQNA LFKELEQAKE WVLTLLEDEG FESFMIDSLD GAKDAQLIKK 

        70         80         90        100        110        120 
AYAFLCLGGD GTILGALRMT HAHNKPCFGV RIGNLGFLSA VELNGLKDFL QDLKQNRIKL 

       130        140        150        160        170        180 
EEHLALEGRI GNTSFYAINE IVIAKKKALG VLDIKACAGH TPFNTYKGDG LIIATPLGST 

       190        200        210        220        230        240 
AYNLSAHGPI VHALSQSYIL TPLCDFSLTQ RPLVLGAEFC LSFCAHEDAL VVIDGQATYD 

       250        260        270        280 
LKANQPLYIQ KSPTTTKLLQ KNSRDYFKVL KEKLLWGESP NKKR 

« Hide

References

[1]"Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori."
Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D. expand/collapse author list , Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.
Nature 397:176-180(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J99 / ATCC 700824.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001439 Genomic DNA. Translation: AAD07009.1. Different initiation.
PIRA71807.
RefSeqNP_224151.1. NC_000921.1.

3D structure databases

ProteinModelPortalQ9ZJ81.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85963.jhp1433.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07009; AAD07009; jhp_1433.
GeneID889455.
KEGGhpj:jhp1433.
PATRIC20607760. VBIHelPyl98156_1558.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
KOK00858.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycHPYL85963:GJB9-1473-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_HELPJ
AccessionPrimary (citable) accession number: Q9ZJ81
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 4, 2001
Last modified: July 9, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families