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Protein

Endoribonuclease AbiQ

Gene

abiQ

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a type III toxin-antitoxin (TA) module. An endoribonuclease that is probably sequence-specific. It is neutralized by its cognate antitoxin RNA AntiQ, which has 2.8 35 nucleotide-long repeats. Cannot be cloned in L.lactis subsp. cremoris strain NZ9000 in the absence of the antitoxin gene; expression in strain NZ9000 even in the presence of antiQ inhibits growth in a bacteriostatic fashion (PubMed:23279123). Confers resistance to 936 and c2 phages but not P335 phages in L.lactis, causes an abortive infection (Abi phenotype). Viral DNA is replicated but not cleaved from its concatemeric form, while the viral major structural protein is produced normally in the presence of this protein (PubMed:9835558). Operon expression in E.coli confers resistance to 3 phages of the Myoviridae family (T4, RB69 and phage 2) and 1 of the Siphoviridae family (T5), but not other tested phages (T1, T3, lambda vir, HK97, Mu and pilH alpha). The presence of this operon in L.lactis subsp. lactis strain IL1403 during phage P008 infection alters the viral transcription profiles (PubMed:23813728).3 Publications

GO - Molecular functioni

  • endoribonuclease activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Toxin

Keywords - Biological processi

Antiviral defense

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease AbiQ1 Publication (EC:3.1.-.-)
Short name:
ABIQ1 Publication
Alternative name(s):
Toxin AbiQCurated
Gene namesi
Name:abiQ1 Publication
Encoded oniPlasmid pSRQ9002 Publications
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Loss of resistance to bacteriophage.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381Y → A or C: Loss of resistance of phage P0008, still has RNase activity. 1 Publication
Mutagenesisi60 – 601P → L: Loss of resistance of phage P0008, slightly decreased RNase activity. 1 Publication
Mutagenesisi62 – 621S → L: Loss of resistance of phage P0008, loss of RNase activity. 1 Publication
Mutagenesisi62 – 621S → T: Loss of resistance of phage P0008, still has RNase activity. 1 Publication
Mutagenesisi63 – 631S → A: Loss of resistance of phage P0008, slightly decreased RNase activity. 1 Publication
Mutagenesisi65 – 651K → A: Loss of resistance of phage P0008, still has RNase activity. 1 Publication
Mutagenesisi66 – 661K → A: Wild-type; retains resistance to phage P0008, still has RNase activity. 1 Publication
Mutagenesisi71 – 711K → A: Loss of resistance of phage P0008, still has RNase activity. 1 Publication
Mutagenesisi78 – 781R → E: Loss of resistance of phage P0008, decreased RNase activity. 1 Publication
Mutagenesisi80 – 801R → E: Wild-type; retains resistance to phage P0008, still has RNase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 183183Endoribonuclease AbiQPRO_0000432894Add
BLAST

Expressioni

Inductioni

Constitutively expressed in the presence or absence of phage P0008 (in L.lactis subsp. lactis strain IL1403) (at protein level); in the presence of phage the abiQ transcript level decreases while the antiQ transcript does not. Part of the antiQ-abiQ operon.1 Publication

Interactioni

Subunit structurei

Forms a triangular heterohexamer with a single 35-nt-long repeat of RNA antitoxin AntiQ.By similarity

Structurei

Secondary structure

1
183
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 195Combined sources
Helixi21 – 277Combined sources
Turni28 – 303Combined sources
Beta strandi44 – 518Combined sources
Beta strandi53 – 6311Combined sources
Helixi69 – 779Combined sources
Turni78 – 814Combined sources
Beta strandi82 – 876Combined sources
Beta strandi90 – 9910Combined sources
Helixi100 – 1023Combined sources
Helixi108 – 1103Combined sources
Beta strandi111 – 1133Combined sources
Helixi121 – 13515Combined sources
Helixi137 – 15216Combined sources
Helixi157 – 1626Combined sources
Helixi166 – 17712Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GLKX-ray2.16A13-183[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ToxN/AbiQ toxin family.Curated

Phylogenomic databases

KOiK19167.

Family and domain databases

InterProiIPR025911. ToxN/AbiQ_toxin.
[Graphical view]
PfamiPF13958. ToxN_toxin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZJ19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFFYKEIL RMTLRFFTVT DEYIAYLRKF ESKVHYQYEN NASTYVGVVL
60 70 80 90 100
KKNDFNYFIP LSSYKKGNPE KDKAMKKRSR IVTRLFEIGN INNPLGYLLH
110 120 130 140 150
HNMIPVPDSE LIPLPLDLKK PKHKMMQKQL IYMKSISEKI ENKSEVVYRK
160 170 180
AAHEKDGYYL KFSCDFKLLE AKATLYSKKS TFQ
Length:183
Mass (Da):21,723
Last modified:May 1, 1999 - v1
Checksum:iFC032C690AF15B35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001314 Genomic DNA. Translation: AAC98713.1.
RefSeqiNP_862552.1. NC_004959.1.

Genome annotation databases

GeneIDi1450204.
KEGGipg:1450204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001314 Genomic DNA. Translation: AAC98713.1.
RefSeqiNP_862552.1. NC_004959.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GLKX-ray2.16A13-183[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1450204.
KEGGipg:1450204.

Phylogenomic databases

KOiK19167.

Family and domain databases

InterProiIPR025911. ToxN/AbiQ_toxin.
[Graphical view]
PfamiPF13958. ToxN_toxin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "AbiQ, an abortive infection mechanism from Lactococcus lactis."
    Emond E., Dion E., Walker S.A., Vedamuthu E.R., Kondo J.K., Moineau S.
    Appl. Environ. Microbiol. 64:4748-4756(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: W-37.
    Plasmid: pSRQ900
  2. "DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanisms."
    Boucher I., Emond E., Parrot M., Moineau S.
    J. Dairy Sci. 84:1610-1620(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: W-37.
    Plasmid: pSRQ900
  3. "Effect of the abortive infection mechanism and type III toxin/antitoxin system AbiQ on the lytic cycle of Lactococcus lactis phages."
    Samson J.E., Belanger M., Moineau S.
    J. Bacteriol. 195:3947-3956(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Structure and activity of AbiQ, a lactococcal endoribonuclease belonging to the type III toxin-antitoxin system."
    Samson J.E., Spinelli S., Cambillau C., Moineau S.
    Mol. Microbiol. 87:756-768(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 13-183, FUNCTION, INDUCTION, MUTAGENESIS OF TYR-38; PRO-60; SER-62; SER-63; LYS-65; LYS-66; LYS-71; ARG-78 AND ARG-80.

Entry informationi

Entry nameiABIQ_LACLL
AccessioniPrimary (citable) accession number: Q9ZJ19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2015
Last sequence update: May 1, 1999
Last modified: June 24, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-12 is the initiator.1 Publication

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.