Reviewed, UniProtKB/Swiss-Prot Q9ZHI1 (THIL_CHRVO)

Names and origin

Protein names

Recommended name:
    Acetyl-CoA acetyltransferase
    EC=2.3.1.9
Alternative name(s):
    Acetoacetyl-CoA thiolase

Gene names

Name:	phaA
Ordered Locus Names:	CV_2790

Organism

Chromobacterium violaceum [Complete proteome] [HAMAP]

Taxonomic identifier

536 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Chromobacterium

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the production of polyhydroxyalkonic acids (PHAs), composed primarily of 3-hydroxybutyric acid (3HB) and 3-hydroxyvaleric acid (3HV).
Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	PHA biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	poly(3-hydroxyalkanoate) biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Acetyl-CoA acetyltransferase		PRO_0000206420
Sites
	Active site	87	1	Acyl-thioester intermediate By similarity
	Active site	348	1	Proton acceptor By similarity
	Active site	378	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	9 – 11	3	QRT → HAP in AAC69616. Ref.2
	Sequence conflict	122	1	H → Y in AAC69616. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9ZHI1-1 [UniParc]. Last modified October 24, 2003. Version 2. Checksum: CCE21993747EF8DB	FASTA	392	40,119
	10 20 30 40 50 60 MEVAIVAAQR TAIGSFGGGL AKIPAPELGA TVIKALLEKT GVKPEDVSEV ILGQVLTAGS 70 80 90 100 110 120 GQNPARQALI KAGLPVTTPA TTLNVVCGSG LRAVHLAAQA ILAGDADIVI AGGQESMSLS 130 140 150 160 170 180 PHILPGSRDG FRMGNAQLVD TMVNDGLTDA YNAYHMGITA ENVAAKYGIG REEQDAFSLQ 190 200 210 220 230 240 SQQRAAAAQK AGKFRDEIVP VLVPQRKGDP LAFDADEFIK HDASADGLAK LRPAFKKDGT 250 260 270 280 290 300 VTAGNASGIN DGAAAVLLMS TQKADQLGLK PLAIIKGYAL TGCEPEIMGI GPVSATRKAL 310 320 330 340 350 360 SKAGWTVEDL DLVEANEAFA AQALGVAKEL GWGSDKVNVN GGAIALGHPI GASGCRVLVT 370 380 390 LLHEMQRRGA KKGLATLCIG GGMGVALAVE RP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability." Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T., Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R., Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F., Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S., Belo A. , van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M., Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P., Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S., Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N., Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P., Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R., Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B., Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J., Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z., de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F., Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A., Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C., Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M., Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C., Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R., Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M., Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R., Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G. Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003) [PubMed: 14500782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12472 / DSM 30191 / IFO 12614 / JCM 1249 / NCIB 9131.
[2]	"Cloning, molecular analysis, and expression of the polyhydroxyalkanoic acid synthase (phaC) gene from Chromobacterium violaceum." Kolibachuk D., Miller A., Dennis D. Appl. Environ. Microbiol. 65:3561-3565(1999) [PubMed: 10427049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-143. Strain: ATCC 12472 / DSM 30191 / IFO 12614 / JCM 1249 / NCIB 9131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016825 Genomic DNA. Translation: AAQ60458.1. AF061446 Genomic DNA. Translation: AAC69616.1.
RefSeq	NP_902460.1.
3D structure databases
SMR	Q9ZHI1. Positions 3-390.
ModBase	Search...
Genome annotation databases
GeneID	2547100.
GenomeReviews	Gene locus CV_2790 in contig AE016825_GR.
KEGG	cvi:CV_2790.
NMPDR	fig|243365.1.peg.2790.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG370930.
OMA	DPATMGM.
PhylomeDB	Q9ZHI1.
Enzyme and pathway databases
BioCyc	CVIO243365:CV_2790-MONOMER.
BRENDA	2.3.1.9. 415.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIL_CHRVO

Accession

Primary (citable) accession number: Q9ZHI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	October 24, 2003
Last modified:	February 9, 2010
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)