Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9ZHG9

- Q9ZHG9_SYNY4

UniProt

Q9ZHG9 - Q9ZHG9_SYNY4

Protein
Submitted name:

L-cysteine/cystine lyase C-DES

Gene

c-des

Organism
Synechocystis sp. (strain PCC 6714) (Aphanocapsa sp. (strain PCC 6714))
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cofactori

    Pyridoxal phosphate.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei114 – 1141Proton donor/acceptor
    Sitei114 – 1141Important for catalytic activityImported
    Sitei197 – 1971Important for catalytic activityImported
    Sitei199 – 1991Important for catalytic activity
    Sitei200 – 2001Important for catalytic activity
    Binding sitei223 – 2231N6-(pyridoxalphosphate)lysine 1 (covalent)Imported
    Sitei223 – 2231Important for catalytic activityImported
    Binding sitei251 – 2511N6-(pyridoxalphosphate)lysine 2Imported
    Binding sitei276 – 2761N6-(pyridoxalphosphate)lysine 2Imported
    Binding sitei360 – 3601Substrate

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. pyridoxal phosphate binding Source: InterPro

    Keywords - Molecular functioni

    LyaseImported

    Keywords - Ligandi

    Pyridoxal phosphateSAAS annotation

    Enzyme and pathway databases

    SABIO-RKQ9ZHG9.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    L-cysteine/cystine lyase C-DESImported
    Gene namesi
    Name:c-desImported
    OrganismiSynechocystis sp. (strain PCC 6714) (Aphanocapsa sp. (strain PCC 6714))Imported
    Taxonomic identifieri1147 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ELQX-ray1.80A/B4-393[»]
    1ELUX-ray1.55A/B4-393[»]
    1N2TX-ray2.00A/B9-393[»]
    1N31X-ray2.20A/B9-393[»]
    ProteinModelPortaliQ9ZHG9.
    SMRiQ9ZHG9. Positions 13-393.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9ZHG9.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.UniRule annotation

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000192. Aminotrans_V/Cys_dSase.
    IPR020578. Aminotrans_V_PyrdxlP_BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00266. Aminotran_5. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ZHG9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADPVNLIPD RHQFPGLANK TYFNFGGQGI LPTVALEAIT AMYGYLQENG    50
    PFSIAANQHI QQLIAQLRQA LAETFNVDPN TITITDNVTT GCDIVLWGLD 100
    WHQGDEILLT DCEHPGIIAI VQAIAARFGI TYRFFPVAAT LNQGDAAAVL 150
    ANHLGPKTRL VILSHLLWNT GQVLPLAEIM AVCRRHQGNY PVRVLVDGAQ 200
    SAGSLPLDFS RLEVDYYAFT GHKWFAGPAG VGGLYIHGDC LGEINPTYVG 250
    WRSITYGAKG EPTGWAEGGK RFEVATSAYP QYAGLLAALQ LHQRQGTAEE 300
    RYQAICQRSE FLWRGLNQLP HVHCLATSAP QAGLVSFTVD SPLGHRAIVQ 350
    KLEEQRIYLR TIADPDCIRA CCHYITDEEE INHLLARLAD FGP 393
    Length:393
    Mass (Da):43,154
    Last modified:May 1, 1999 - v1
    Checksum:i4AC0ADA899A3264C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061964 Genomic DNA. Translation: AAD02935.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061964 Genomic DNA. Translation: AAD02935.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ELQ X-ray 1.80 A/B 4-393 [» ]
    1ELU X-ray 1.55 A/B 4-393 [» ]
    1N2T X-ray 2.00 A/B 9-393 [» ]
    1N31 X-ray 2.20 A/B 9-393 [» ]
    ProteinModelPortali Q9ZHG9.
    SMRi Q9ZHG9. Positions 13-393.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK Q9ZHG9.

    Miscellaneous databases

    EvolutionaryTracei Q9ZHG9.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR000192. Aminotrans_V/Cys_dSase.
    IPR020578. Aminotrans_V_PyrdxlP_BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00266. Aminotran_5. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel L-cysteine/cystine C-S-lyase directing [2Fe-2S] cluster formation of Synechocystis ferredoxin."
      Leibrecht I., Kessler D.
      J. Biol. Chem. 272:10442-10447(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: PCC 6714Imported.
    2. "Evidence for cysteine persulfide as reaction product of L-Cyst(e)ine C-S-lyase (C-DES) from Synechocystis. Analyses using cystine analogues and recombinant C-DES."
      Lang T., Kessler D.
      J. Biol. Chem. 274:189-195(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: PCC 6714Imported.
    3. "Crystal structure of the cystine C-S lyase from Synechocystis: stabilization of cysteine persulfide for FeS cluster biosynthesis."
      Clausen T., Kaiser J.T., Steegborn C., Huber R., Kessler D.
      Proc. Natl. Acad. Sci. U.S.A. 97:3856-3861(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 4-393 IN COMPLEX WITH N6-(PYRIDOXALPHOSPHATE)LYSINE, ACTIVE SITE.
    4. "Snapshots of the cystine lyase C-DES during catalysis. Studies in solution and in the crystalline state."
      Kaiser J.T., Bruno S., Clausen T., Huber R., Schiaretti F., Mozzarelli A., Kessler D.
      J. Biol. Chem. 278:357-365(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 9-393 IN COMPLEX WITH N6-(PYRIDOXALPHOSPHATE)LYSINE, ACTIVE SITE.

    Entry informationi

    Entry nameiQ9ZHG9_SYNY4
    AccessioniPrimary (citable) accession number: Q9ZHG9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 1, 1999
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3