Q9ZHG4 (DPO3A_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit alpha
EC=2.7.7.7

Gene names

Name:	dnaE
Ordered Locus Names:	TM_0461

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	842 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 842	842	DNA polymerase III subunit alpha		PRO_0000103355
Experimental info
	Sequence conflict	377	1	I → M in AAC80434. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9ZHG4 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 19BB813C8085C277	FASTA	842	96,500
	10 20 30 40 50 60 MIPWVISPYS FDGSVVRFEK LALLLKRKGL KSVILADRNF HAAVKFNTIM RKHGLIPVHG 70 80 90 100 110 120 LWKDGRIFVA RNREEFDSLV RYYNGETHEI EDIPVFQESE LTPVRYLDAS EKKASIFMRK 130 140 150 160 170 180 IFGLDEDVQG FPEKCEDVAD ILNAEAYDLR VNHRFPTPPK NWNELLIKKA EPLGEEYISR 190 200 210 220 230 240 LKRELEVIKR KGFTPYIYTV EKVVEIAKKM GIKVGPGRGS AVGSLVAYLC GITEVDPIKY 250 260 270 280 290 300 DLLFERFLNE ERQEPPDIDV DVEDRRRKDL IKELSKSFQV YQVSTFGNLT EKSLKNLINS 310 320 330 340 350 360 VLPDASLEEK NEIYKTVYGL PHHPSVHAAG VVISENPLPL PTRTEEDIPI TDYDMYDLQE 370 380 390 400 410 420 IGVVKIDILG LKTLSFIKDF KKEIFDYSDE KTYHLISKGK TLGVFQLEGL QARKLCRRIS 430 440 450 460 470 480 PRNMDELSIL LALNRPGPLR SGLDVMFSNS KNVPAFFRKM FPETRGVLIY QEQIMRLAMF 490 500 510 520 530 540 AGLSGTEADI LRRAIAKKER EKMEPLLEKM KKGLLEKGME NAEQILEILL NFSSYAFNKS 550 560 570 580 590 600 HSVAYAHITY QTAYLKAHHL EEFFKLYFAY NSSDAGKIFL AVQELRNEGY RVHPPDINIS 610 620 630 640 650 660 GKDLVFHGKD VYLPLTVVKG VGVTLVEQIE KIRPVSSVRE LQERVTGVPR NVVESLITAG 670 680 690 700 710 720 AFDKLYENRK LALEELNKRV EKDILEIRSL FGEKVEQESS NIKIGDITEL EEKSMGFPLT 730 740 750 760 770 780 PVHEVPTGLF ARIDDVFTYG RILPVLVKRV SRNIVTDGLS VCRVRTDVPD GVHLVLLSPL 790 800 810 820 830 840 QKIIKIWPFN ENTRFVYRVD FTATLEKAGQ NEITEVLKNG AVVRYEGYRP LTDEYRYRVV PR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The hyperthermophilic bacterium Thermotoga maritima has two different classes of family C DNA polymerases: evolutionary implications." Huang Y.P., Ito J. Nucleic Acids Res. 26:5300-5309(1998) [PubMed: 9826752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF063188 Genomic DNA. Translation: AAC80434.1. AE000512 Genomic DNA. Translation: AAD35546.1.
PIR	E72373.
RefSeq	NP_228271.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q9ZHG4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	897492.
GenomeReviews	Gene locus TM_0461 in contig AE000512_GR.
KEGG	tma:TM0461.
NMPDR	fig|243274.1.peg.455.
PATRIC	23935819. VBITheMar51294_0468.
TIGR	TM_0461.
Phylogenomic databases
HOGENOM	HBG477044.
OMA	AYAHITY.
PhylomeDB	Q9ZHG4.
ProtClustDB	CLSK875157.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_0461-MONOMER.
Family and domain databases
InterPro	IPR011708. DNA_pol3_alpha. IPR003141. Pol/His_phosphatase_N. [Graphical view]
KO	K02337.
Pfam	PF07733. DNA_pol3_alpha. 2 hits. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPO3A_THEMA

Accession

Primary (citable) accession number: Q9ZHG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program