DNA polymerase III polC-type - Thermotoga maritima

Preferred order of sections

Names and origin

Protein names

Recommended name:
DNA polymerase III polC-type
Short name=PolIII
EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	TM_0576

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	1367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity. HAMAP MF_00356
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00356
Subcellular location	Cytoplasm By similarity HAMAP MF_00356.
Sequence similarities	Belongs to the DNA polymerase type-C family. PolC subfamily. Contains 1 exonuclease domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1367

1367

DNA polymerase III polC-type HAMAP MF_00356

PRO_0000204605

Regions

Domain

358 – 513

156

Exonuclease

Secondary structure

1

.

1367

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9ZHF6 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: EE5916FA70591F84
Show »

FASTA

1,367

155,363

        10         20         30         40         50         60 
MKKIENLKWK NVSFKSLEID PDAGVVLVSV EKFSEEIEDL VRLLEKKTRF RVIVNGVQKS 

        70         80         90        100        110        120 
NGDLRGKILS LLNGNVPYIK DVVFEGNRLI LKVLGDFARD RIASKLRSTK KQLDELLPPG 

       130        140        150        160        170        180 
TEIMLEVVEP PEDLLKKEVP QPEKREEPKG EELKIEDENH IFGQKPRKIV FTPSKIFEYN 

       190        200        210        220        230        240 
KKTSVKGKIF KIEKIEGKRT VLLIYLTDGE DSLICKVFND VEKVEGKVSV GDVIVATGDL 

       250        260        270        280        290        300 
LLENGEPTLY VKGITKLPEA KRMDKSPVKR VELHAHTKFS DQDAITDVNE YVKRAKEWGF 

       310        320        330        340        350        360 
PAIALTDHGN VQAIPYFYDA AKEAGIKPIF GIEAYLVSDV EPVIRNLSDD STFGDATFVV 

       370        380        390        400        410        420 
LDFETTGLDP QVDEIIEIGA VKIQGGQIVD EYHTLIKPSR EISRKSSEIT GITQEMLENK 

       430        440        450        460        470        480 
RSIEEVLPEF LGFLEDSIIV AHNANFDYRF LRLWIKKVMG LDWERPYIDT LALAKSLLKL 

       490        500        510        520        530        540 
RSYSLDSVVE KLGLGPFRHH RALDDARVTA QVFLRFVEMM KKIGITKLSE MEKLKDTIDY 

       550        560        570        580        590        600 
TALKPFHCTI LVQNKKGLKN LYKLVSDSYI KYFYGVPRIL KSELIENREG LLVGSACISG 

       610        620        630        640        650        660 
ELGRAALEGA SDSELEEIAK FYDYIEVMPL DVIAEDEEDL DRERLKEVYR KLYRIAKKLN 

       670        680        690        700        710        720 
KFVVMTGDVH FLDPEDARGR AALLAPQGNR NFENQPALYL RTTEEMLEKA IEIFEDEEIA 

       730        740        750        760        770        780 
REVVIENPNR IADMIEEVQP LEKKLHPPII ENADEIVRNL TMKRAYEIYG DPLPEIVQKR 

       790        800        810        820        830        840 
VEKELNAIIN HGYAVLYLIA QELVQKSMSD GYVVGSRGSV GSSLVANLLG ITEVNPLPPH 

       850        860        870        880        890        900 
YRCPECKYFE VVEDDRYGAG YDLPNKNCPR CGAPLRKDGH GIPFETFMGF EGDKVPDIDL 

       910        920        930        940        950        960 
NFSGEYQERA HRFVEELFGK DHVYRAGTIN TIAERSAVGY VRSYEEKTGK KLRKAEMERL 

       970        980        990       1000       1010       1020 
VSMITGVKRT TGQHPGGLMI IPKDKEVYDF TPIQYPANDR NAGVFTTHFA YETIHDDLVK 

      1030       1040       1050       1060       1070       1080 
IDALGHDDPT FIKMLKDLTG IDPMTIPMDD PDTLAIFSSV KPLGVDPVEL ESDVGTYGIP 

      1090       1100       1110       1120       1130       1140 
EFGTEFVRGM LVETRPKSFA ELVRISGLSH GTDVWLNNAR DWINLGYAKL SEVISCRDDI 

      1150       1160       1170       1180       1190       1200 
MNFLIHKGME PSLAFKIMEN VRKGKGITEE MESEMRRLKV PEWFIESCKR IKYLFPKAHA 

      1210       1220       1230       1240       1250       1260 
VAYVSMAFRI AYFKVHYPLQ FYAAYFTIKG DQFDPVLVLR GKEAIKRRLR ELKAMPAKDA 

      1270       1280       1290       1300       1310       1320 
QKKNEVSVLE VALEMILRGF SFLPPDIFKS DAKKFLIEGN SLRIPFNKLP GLGDSVAESI 

      1330       1340       1350       1360 
IRAREEKPFT SVEDLMKRTK VNKNHIELMK SLGVLGDLPE TEQFTLF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The hyperthermophilic bacterium Thermotoga maritima has two different classes of family C DNA polymerases: evolutionary implications." Huang Y.P., Ito J. Nucleic Acids Res. 26:5300-5309(1998) [PubMed: 9826752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF065313 Genomic DNA. Translation: AAC80438.1.
AE000512 Genomic DNA. Translation: AAD35661.1.

PIR

C72360.

RefSeq

NP_228386.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2P1J	X-ray	2.50	A/B	347-522	[»]

ProteinModelPortal

Q9ZHF6.

SMR

Q9ZHF6. Positions 357-520.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

897644.

GenomeReviews

Gene locus TM_0576 in contig AE000512_GR.

KEGG

tma:TM0576.

NMPDR

fig|243274.1.peg.570.

PATRIC

23936061. VBITheMar51294_0585.

TIGR

TM_0576.

Phylogenomic databases

HOGENOM

HBG617116.

OMA

WKTTHFD.

PhylomeDB

Q9ZHF6.

ProtClustDB

PRK00448.

Enzyme and pathway databases

BioCyc

TMAR243274:TM_0576-MONOMER.

Family and domain databases

HAMAP

MF_00356. DNApol_PolC.
[Tree]

InterPro

IPR023223. DNA_pol3-C_alpha-helix.
IPR011708. DNA_pol3_alpha.
IPR006054. DnaQ.
IPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR006308. PolC_gram_pos.
IPR012337. RNaseH-like_dom.
[Graphical view]

Gene3D

G3DSA:1.20.5.140. Prot_inh_IA3_yeast. 1 hit.

KO

K03763.

Pfam

PF07733. DNA_pol3_alpha. 1 hit.
PF02811. PHP. 1 hit.
PF00929. RNase_T. 1 hit.
[Graphical view]

SMART

SM00479. EXOIII. 1 hit.
SM00481. POLIIIAc. 1 hit.
[Graphical view]

SUPFAM

SSF53098. RNaseH_fold. 1 hit.

TIGRFAMs

TIGR00573. Dnaq. 1 hit.
TIGR01405. PolC_Gram_pos. 1 hit.

ProtoNet

Search...

Entry information

Entry name

DPO3_THEMA

Accession

Primary (citable) accession number: Q9ZHF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families