Reviewed, UniProtKB/Swiss-Prot Q9ZHD7 (SYM_BUCAP)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BUsg_102

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Monomer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000139112
Regions
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00098
	Motif	332 – 336	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	146	1	Zinc By similarity
	Metal binding	149	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Metal binding	162	1	Zinc By similarity
	Binding site	335	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZHD7-1 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 77F79377EF21492A	FASTA	545	63,822
	10 20 30 40 50 60 MSNKFKKILV TCALPYANGP IHIGHMLEHI QADIWVRYQR MRNNEVWFIS SDDAHGTAIM 70 80 90 100 110 120 LKSEKLGITP IKLIKKIKEE HIIDFSNFNI SHDNYHSTHC VENLFLLRKI FLSLSEQKLI 130 140 150 160 170 180 NEKKIFQFYD NTKKMFLPDR FIKGTCPFCS SKNQNGDNCE ICGSIYEPTD LVNPISVISN 190 200 210 220 230 240 SVPILKNTTH LYFNLPLFTN MLNKWIHSGI LEKSVAKKTE EWLKSGLKEW GISRDAPYFG 250 260 270 280 290 300 FKIPKFDKKY FYVWLDAPVG YISAFKNFCT KNKKVDFNEF WKKESKCELY HFIGKDIIYF 310 320 330 340 350 360 HTLFWPAILE ASSYRKPNGI FVHGHLTING LKLSKSRGFL IKASDWIKCF DSDSLRYYYA 370 380 390 400 410 420 SKLSNNINDI EINLEDFIQK INSDIVNKLV NLASRNASFI HKYFDGYLAD SLGDCNLYQD 430 440 450 460 470 480 FIDISKKIAN FFENRQFSSV IRECMKLLDL ANQYINQREP WKIKQDNFNK LHTICTVGIN 490 500 510 520 530 540 LFRLVVIFLK PIIPDLAKKT EYFLISDLTW KGIDKPLLSH KIKKFKKLYN RINHDKILQL SLLCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Buchnera aphidicola (Aphid endosymbiont) contains genes encoding enzymes of histidine biosynthesis." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 37:356-358(1998) [PubMed: 9767718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-234.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67672.1. AF067228 Genomic DNA. Translation: AAC97364.1.
RefSeq	NP_660461.1.
3D structure databases
SMR	Q9ZHD7. Positions 6-537.
ModBase	Search...
Genome annotation databases
GeneID	1005919.
GenomeReviews	Gene locus BUsg_102 in contig AE013218_GR.
KEGG	bas:BUsg102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG721667.
OMA	DFSWREF.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG102-MONOMER.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BUCAP

Accession

Primary (citable) accession number: Q9ZHD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 30, 2002
Last modified:	February 9, 2010
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)